Pyrroloquinolinequinone enzyme electrode based on the coupling of methanol dehydrogenase to a tetrathiafulvalene-tetracyanoquinodimethane electrode

Zhao, Shikai; Lennox, R. Bruce

doi:10.1021/ac00011a021

Cited by 47 publications

(13 citation statements)

References 28 publications

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“…The TTF-TCNQ -electron transfer complex -along with application to glucose determination is also used to develop biosensors for other substrates, in particular hydrogen peroxide [43], lactate [39,63], fructose [64], glutamate [52], lactulose [65], biological purines [28], dopamine [37], hypoxanthine [40,42,49], acetylcholine and choline [32,66], methanol [53,67], sulfite [41], and formaldehyde [34] ( Table 1). These biosensors operate in the potential range from À 0.075 V to þ 0.30 V vs. SCE, and the potential values usually are limited by the electrochemical decomposition of the mediator complex.…”

Section: Biosensorsmentioning

confidence: 99%

Conductive Organic Complex Salt TTF‐TCNQ as a Mediator for Biosensors. An Overview

et al. 2007

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Preparation and application of a conductive organic salt complex of tetrathiafulvalene-tetracyanoquinodimethane (TTF-TCNQ) for analytical bioelectrochemistry as a mediator is overviewed in this work. The third-generation biosensors based on this charge transfer salt are very promising for biosensors applied in vivo. Such mediated biosensors have been studied mainly for glucose determination, but at present other substrates are being applied in this system more and more often.

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Section: Biosensorsmentioning

confidence: 99%

Conductive Organic Complex Salt TTF‐TCNQ as a Mediator for Biosensors. An Overview

et al. 2007

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“…However, direct electron transfer has been used for several alcohol biosensor applications using enzyme immobilization via e.g. organic salts in silicon oil [76], osmium complexes [77], conducting polymers [78] or thiol layer [79] showing the 21 viability of direct electron transfer with PQQ-based enzymes. In these cases, the electrons mostly do not move directly from PQQ to the electrode but travel through heme-c redox groups inside the enzyme [78].…”

Section: Enzymatic Catalysismentioning

confidence: 99%

Silicon nanograss as micro fuel cell gas diffusion layer

et al. 2010

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Direct methanol fuel cells (DMFC) produce electrical energy directly from chemical energy. They are a promising candidate for power sources of portable devices due to the high energy density of methanol and the quick recharging procedure by fuel insertion. However, the problem areas of the DMFC are the slow electro-oxidation of methanol and the permeated methanol reacting at the cathode. New catalysts are constantly searched but they are often tested only for catalytic activity and the DMFC testing is omitted even though the catalyst layer (CL) structure has a large impact on the performance. Miniaturization of the system is also necessary for portable applications. Silicon etching can be used to fabricate small structures for fuel cells replacing or enhancing the functions of laboratory-scale components.In the first part of this thesis, new catalysts for the DMFC are studied with the emphasis on the CL structure. Different carbon supports for the anode were studied: standard carbon black and alternative few-walled carbon nanotubes (FWCNT) and graphitized carbon nanofiber (GNF). The alternative supports showed better DMFC performance but their stability was lower than with carbon black. However, the CL formed with GNF showed a very porous structure enhancing the mass transfer, so that higher binder content could be used improving the stability to the level of carbon black and the performance by 30%. The FWCNTs were also investigated as a platform for enzymatic methanol oxidation by studying the electrochemical properties of an immobilized cofactor pyrroloquinoline quinone (PQQ). A large amount of PQQ was adsorbed having a strong redox response and good stability in a wide pH window. For the cathode, a methanol-tolerant, Pt-free nitrogen-doped FWCNTs were tested in an alkaline DMFC as such testing is not often made. Its performance was remarkably 4 times better than with Pt when synthetic air was used as the oxidant.In the second part of thesis, an integrated gas diffusion layer (GDL) consisting of Si nanoneedles (nanograss) was tested in a micro fuel cell (MFC). The layer functioned properly at low current densities. For high power applications, a standard carbon cloth GDL was tested with the nanograss as a contact surface reducing the resistance between the GDL and the flow field. The use of the nanograss improved the MFC performance and stability. Finally, the MFCs were used as a catalyst testing platform and the results were compared with a similar test in a laboratory-scale DMFC. The results varied showing that the DMFC components also have a large impact on catalyst testing.Keywords direct methanol fuel cell, carbon nanomaterials, micro fuel cells, catalyst layer Tiivistelmä Suorametanolipolttokennot (SMPK) tuottavat sähköenergiaa suoraan kemiallisesta energiasta. Ne ovat lupaava vaihtoehto kannettavien laitteiden voimanlähteeksi, koska metanolilla on korkea energiatiheys ja lataaminen tapahtuu nopeasti polttoainelisäyksellä. SMPK:lla on kuitenkin rajoitteina metanolin hidas hapetusreaktio ja katodi...

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“…A third class of alcohol dehydrogenases, pyrroloquinoline quinone (PQQ) dependent alcohol dehydrogenases (EC1.1.99.8), has also been employed in biosensor designs for ethanol [18][19][20][21][22][23][24][25][26][27][28][29][30]. In contrast, methanol dehydrogenase (MDH) from which PQQ was first purified [31] has been rarely used in sensing applications [27,32].…”

Section: Introductionmentioning

confidence: 99%

Amperometric detection of methanol with a methanol dehydrogenase modified electrode sensor

Liu

Kirchhoff

2007

Journal of Electroanalytical Chemistry

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Pyrroloquinolinequinone enzyme electrode based on the coupling of methanol dehydrogenase to a tetrathiafulvalene-tetracyanoquinodimethane electrode

Cited by 47 publications

References 28 publications

Conductive Organic Complex Salt TTF‐TCNQ as a Mediator for Biosensors. An Overview

Conductive Organic Complex Salt TTF‐TCNQ as a Mediator for Biosensors. An Overview

Silicon nanograss as micro fuel cell gas diffusion layer

Amperometric detection of methanol with a methanol dehydrogenase modified electrode sensor

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