2020
DOI: 10.15252/msb.20199270
|View full text |Cite
|
Sign up to set email alerts
|

Pyruvate kinase variant of fission yeast tunes carbon metabolism, cell regulation, growth and stress resistance

Abstract: Cells balance glycolysis with respiration to support their metabolic needs in different environmental or physiological contexts. With abundant glucose, many cells prefer to grow by aerobic glycolysis or fermentation. Using 161 natural isolates of fission yeast, we investigated the genetic basis and phenotypic effects of the fermentationrespiration balance. The laboratory and a few other strains depended more on respiration. This trait was associated with a single nucleotide polymorphism in a conserved region o… Show more

Help me understand this report
View preprint versions

Search citation statements

Order By: Relevance

Paper Sections

Select...
3
1
1

Citation Types

5
31
0

Year Published

2020
2020
2023
2023

Publication Types

Select...
7
1

Relationship

3
5

Authors

Journals

citations
Cited by 29 publications
(39 citation statements)
references
References 134 publications
(159 reference statements)
5
31
0
Order By: Relevance
“…The observed relationship between PYK1 and metabolism aligns with a recent genome-wide association study (GWAS) that found PYK1 as a key regulator of Schizosaccharomyces pombe yeast growth when oxidative phosphorylation is blocked ( Kamrad et al, 2020 ). Specifically, T343A mutant yeast, which have greater PYK1 activity, are more resistant to the growth inhibiting effects of antimycin A, a mitochondrial complex III inhibitor ( Kamrad et al, 2020 ). This suggests that “A” mutant yeast rely less on respiration.…”
Section: Discussionsupporting
confidence: 84%
See 1 more Smart Citation
“…The observed relationship between PYK1 and metabolism aligns with a recent genome-wide association study (GWAS) that found PYK1 as a key regulator of Schizosaccharomyces pombe yeast growth when oxidative phosphorylation is blocked ( Kamrad et al, 2020 ). Specifically, T343A mutant yeast, which have greater PYK1 activity, are more resistant to the growth inhibiting effects of antimycin A, a mitochondrial complex III inhibitor ( Kamrad et al, 2020 ). This suggests that “A” mutant yeast rely less on respiration.…”
Section: Discussionsupporting
confidence: 84%
“…This suggests that “A” mutant yeast rely less on respiration. Indeed, the researchers found that yeast strains with the “A” mutation compared to the wildtype “T” show greater glucose uptake and lower oxygen consumption ( Kamrad et al, 2020 ). Thus, pairing our result with recent research shows that either increasing or decreasing PYK1 activity alters fermentation, cementing the role of PYK1 in promoting fermentation in yeast.…”
Section: Discussionmentioning
confidence: 99%
“…Some fission yeast isolates, including the standard laboratory strain, possess a mutation in pyruvate kinase, a key glycolytic enzyme (Kamrad et al , 2020). This feature limits glycolytic flux and causes increased respiration during fermentative growth (Kamrad et al , 2020). It is possible that the shortage of amino acids is exacerbated by this mutation.…”
Section: Discussionmentioning
confidence: 99%
“…However, such adaptations are the exception: most yeast species, including fission yeast ( Schizosaccharomyces pombe ), are petite‐negative and do not cope well under anaerobic conditions (Heslot et al , 1970; Chiron et al , 2007). The S. pombe laboratory strain is part of a group of natural isolates that show higher respiration during fermentative growth, which is caused by a naturally occurring mutation in pyruvate kinase that limits glycolytic flux (Kamrad et al , 2020). We have also reported that respiration is required for rapid cell proliferation during fermentation (Malecki et al , 2016).…”
Section: Introductionmentioning
confidence: 99%
“…The increased respiration rate at the fermentation phase in S. pombe is associated with low activity of a sole enzyme, pyruvate kinase 1 (Pyk1), a protein that forms a homo-tetramer during glycolysis to convert phosphoenolpyruvate to pyruvate, the input for aerobic respiration (TCA cycle). Accelerated Pyk1 activity restricts the respiration at the fermentation phase and leads to changes in cellular metabolism and physiology, most notably sensitivity to oxidative stress [ 129 ]. Mitochondrial respiration accompanied by the formation of ROS in the fermentation phase of S. pombe , correlates with the requirement of NEDD8/Rub1 for viability.…”
Section: Nedd8/rub1 and The Respiration Lifestyle Of Various Fungal Smentioning
confidence: 99%