The rate of CO2 assimilation and levels of metabolites of the C4 cycle and reductive pentose phosphate pathway in attached leaves of maize (Zea mays L.) were (17,18,25). However, recent reports revealed that there were up to 30-fold differences in PEP carboxylase activity during dark to light transitions in maize when assayed in simulated conditions of dark or light or in the presence of effectors (5, 13). Studies of metabolite levels during induction in maize leaves (9, 20, 28) provided a basis for in vitro simulation ofconditions in vivo. The total level of the C4 cycle intermediates (malate, aspartate, pyruvate, PEP, and alanine) increased during induction (28) and also with increasing CO2 concentration (31,32) as the photosynthesis rate increased. These findings suggested that input of carbon into the C4 cycle and an increase in the total level of the C4 cycle intermediates is required to increase the photosynthetic rate in maize leaves (9,28,31,32).Although the influence of light intensity on metabolite levels in C4 plants has not been reported, this is an important environmental factor controlling C4 photosynthesis. Thus, the purpose of this study was to measure changes in photosynthetic metabolite levels in maize leaves under steady-state photosynthesis with varying light intensities.Recently, studies have been conducted on changes in photosynthetic metabolite levels during the induction period (9,20,28), and under steady-state photosynthesis with normal atmospheric conditions (19, 26) and with varying CO2 concentration (31) in maize leaves. These studies have increased our understanding of C4 photosynthesis and they provide a new approach to research in C4 plant. In part they have shown that there are sufficient concentration gradients between the mesophyll and bundle sheath to facilitate intercellular transport of certain C4 cycle intermediates by diffusion during C4 photosynthesis (19,