Pyruvate Protects Against Intestinal Injury by Inhibiting the JAK/STAT Signaling Pathway in Rats With Hemorrhagic Shock

Food Science & Nutrition

et al. 2020

Section: Resultsmentioning

confidence: 99%

Metabolomics study of dried ginger extract on serum and urine in blood stasis rats based on UPLC‐Q‐TOF/MS

Cao

Food Science & Nutrition

et al. 2020

“…Using pyruvate-peritoneal dialysis solution, peritoneal resuscitation protects against intestinal injury by inhibiting the STAT pathway in rats with hemorrhagic shock. 30 Li et al 31 demonstrated that the mTOR/STAT3 signaling pathway is activated by lipopolysaccharide (LPS) followed by the induction of apoptosis and inflammation in rat intestine epithelial cells, and echinacoside could alleviate the toxic effect of LPS. Some downstream targets (cyclin E, cyclin-dependent kinase 2, cyclin D1, and c-Myc) of the STAT pathway were confirmed to play important roles in cell cycle distribution and progression in cutaneous T-cell lymphoma HUT78 cells.…”

Section: Discussionmentioning

confidence: 99%

New Mechanistic Insight into the Protective Effects of Ganoderma lucidum Polysaccharides Against Palmitic Acid-Induced Cell Damage in Porcine Intestinal Epithelial Cell Line IPEC-J2

Liang

Yuan

Natural Product Communications

et al. 2022

Ganoderma lucidum ( G. lucidum) is one of the well-known mushrooms in China, which has G. lucidum polysaccharides (GLP) that have been widely studied for various biological activities, such as antioxidant, antitumor, antiinflammatory, antiviral, antidiabetes, and immunomodulatory activities. A signal transducer and activator of transcription (STAT) signaling pathway is related to cell proliferation and apoptosis. The relationship between STAT and intestinal protection of GLP is still unknown. We studied the inhibitors AG490 in the STAT pathway and its downstream molecules to analyze the unique effects in the protection of GLP against palmitic acid (PA)-induced porcine intestinal epithelial cells (IPEC-J2) injury. Compared to PA treatment, GLP + PA obviously decreased Ca2+ concentration, H2O2 production, NF-E2-related factor 2 (Nrf2) nuclear translocation, STAT1 and STAT2 protein levels, and increased nuclear factor kappa-B (NF-κB) nuclear translocation and p-STAT3/STAT3 ratio in IPEC-J2 cells. After inhibition of STAT3 signaling, p-STAT3/STAT3 ratio, NF-κB nuclear translocation obviously decreased and Nrf2 nuclear translocation significantly increased in the GLP + PA group. The protection of GLP on proliferation and apoptosis of PA-induced IPEC-J2 cells was suppressed by inhibiting STAT3. The STAT3 pathway regulated the enterocyte-protective effects of GLP by modulating the nuclear translocation of Nrf2 and NF-κB. We provide new insights into the mechanism of STAT signaling for the protection of GLP on PA-induced intestinal epithelial cell injury.

“…Pyruvate can stimulate the transcription of broblast growth factor receptors and vascular endothelial growth factor m RNAs and promote the aggregation of new blood vessels in tissues. Studies have shown that pyruvate can reduce intestinal ischemia-reperfusion injury (IRI) and effectively protect internal organs by shortening hypoxemia time, removing oxygen free radicals, inhibiting in ammatory response, increasing pH value and increasing energy production [30]. In this study, compared with the NG, the metabolic pathway of pyruvate in the MG was destroyed, and pyruvate was abnormally increased, indicating that pyruvate level was increased under the condition of disease, and there were differences between the other groups and the MG.…”

Section: Discussionmentioning

confidence: 99%

Metabolomics Study of Dried Ginger Extract on Serum and Urine in Blood Stasis Rats Based on UPLC-Q-TOF/MS

Min

Cao

et al. 2020

Preprint

Backgroud: According to the theory of traditional Chinese medicine (TCM), blood stasis syndrome (BSS) is the basis for many cardiovascular diseases. Ginger is often used as Chinese medicine (herb medicine) in China and Southeast Asia for the treatment of cardiovascular, respiratory, and digestive diseases. but the specific mechanism is unclear.Methods: After establishing an acute rat model of blood stasis syndrome, blood and urine of 5 groups of rats were collected for analysis of efficacy indicators. Aortic vessels are used for pathological testing. Serum and urine were used for metabolomics, multivariate statistical analysis was used to explore metabolites and metabolic pathways, and the correlation between metabolites and pharmacodynamic indicators was further explored.Results: In this experiment, the model of blood stasis syndrome was successfully established based on changes in vascular disease and the efficacy index. The experimental results show that the efficacy indicators of dried ginger (DG) extracts of different doses have different degrees of changes than model group (MG), and the high dose of dried ginger group (GJH) changes are the most significant (p < 0.05 or p < 0.01). And 22 metabolites (10 in serum and 12 in urine) were identified and contributed to the blood stasis progress. These metabolites mainly involve seven metabolism pathways in different impact-value. Dried ginger has therapeutic effects on BSS rats by regulating multiple metabolic pathways.Conclusion: This study provides an effective method for understanding the metabolic mechanism of dried ginger extracts on BSS.