QCM-based aptamer selection and detection of Salmonella typhimurium

Wang, Lijun; Wang, Ronghui; Chen, Fang; Jiang, Tieshan; Wang, Hong; Slavik, Michael F.; Wei, Hua; Li, Yanbin

doi:10.1016/j.foodchem.2016.11.104

Cited by 126 publications

(56 citation statements)

References 32 publications

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“…On the other hand, Wang et al . 37 developed a quartz crystal microbalance-based aptasensor to detect S. typhimurium , which could detect 1.0 × 10 4 cfu/mL of S. typhimurium in less than 1 h. These results confirmed that stringent efforts are still needed to develop more competitive aptasensor-based multiple detection strategies.…”

Section: Resultsmentioning

confidence: 66%

“…The ultimate aim in the development of these sensing devices is to reduce the detection cost and time with improved sensitivity; therefore, this study developed an aptamer-based sensing chip conjugated with gold nanoparticles. A previous study used antibodies conjugated with nanoparticles 36 , which were utilized for pathogen detection; however, use of aptamers has several advantages over antibodies, such as easy labeling, long-term stability, low cost, and recognition of a wide variety of targets, which suggests their potential use as a recognition probe to replace antibodies in the field of diagnostics 37 .Therefore, this study used aptamers conjugated with gold nanoparticles to improve the stability and sensitivity. In addition, the prepared sensing chips were dipped in the test solution and allowed to react for 30 min (the optimal time for bacterial binding, as determined in the previous section).…”

Section: Resultsmentioning

confidence: 99%

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Development of gold nanoparticle-aptamer-based LSPR sensing chips for the rapid detection of Salmonella typhimurium in pork meat

Heo

Shukla̽

et al. 2017

Sci Rep

145

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A non-labeled, portable plasmonic biosensor-based device was developed to enable the ultra-sensitive and selective detection of Salmonella typhimurium in pork meat samples. Specifically, a plasmonic sensor, using the self-assembly of gold nanoparticles (AuNPs) to achieve a regulated diameter of 20 nm for the AuNP monolayers, was used to conduct high-density deposition on a transparent substrate, which produced longitudinal wavelength extinction shifts via a localized surface plasmon resonance (LSPR) signal. The developed aptamers conjugated to the LSPR sensing chips revealed an ultra-sensitive upper limit of detection (LOD) of approximately 104 cfu/mL for S. typhimurium in pure culture under the optimal assay conditions, with a total analysis time of 30–35 min. When the LSPR sensing chips were applied on artificially contaminated pork meat samples, S. typhimurium in the spiked pork meat samples was also detected at an LOD of 1.0 × 104 cfu/mL. The developed method could detect S. typhimurium in spiked pork meat samples without a pre-enrichment step. Additionally, the LSPR sensing chips developed against S. typhimurium were not susceptible to any effect of the food matrix or background contaminant microflora. These findings confirmed that the developed gold nanoparticle-aptamer-based LSPR sensing chips could facilitate sensitive detection of S. typhimurium in food samples.

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Section: Resultsmentioning

confidence: 66%

Section: Resultsmentioning

confidence: 99%

Development of gold nanoparticle-aptamer-based LSPR sensing chips for the rapid detection of Salmonella typhimurium in pork meat

Heo

Shukla̽

et al. 2017

Sci Rep

145

View full text Add to dashboard Cite

show abstract

“…In recent years, various biosensors have been reported for determination of foodborne bacteria, such as electrochemical [1,2], quartz crystal microbalance [3,4] and surface plasmon resonance [5,6], etc. They have attracted great attention because of their simple operation, short time, miniaturized size, and high sensitivity.…”

Section: Introductionmentioning

confidence: 99%

“…After the complexes were captured in the separation chamber using the external magnetic field, GSH was finally injected into the chamber to decompose the MnO 2 NFs into Mn 2+ for releasing the QDs, whose fluorescent intensity was measured by the optical detector to determine the amount of the Salmonella cells. In recent years, various biosensors have been reported for determination of foodborne bacteria, such as electrochemical [1,2], quartz crystal microbalance [3,4] and surface plasmon resonance [5,6], etc. They have attracted great attention because of their simple operation, short time, miniaturized size, and high sensitivity.…”

Section: Introductionmentioning

confidence: 99%

A Microfluidic Biosensor Based on Magnetic Nanoparticle Separation, Quantum Dots Labeling and MnO2 Nanoflower Amplification for Rapid and Sensitive Detection of Salmonella Typhimurium

Huang

et al. 2020

Micromachines

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Screening of foodborne pathogens is an effective way to prevent microbial food poisoning. A microfluidic biosensor was developed for rapid and sensitive detection of Salmonella Typhimurium using quantum dots (QDs) as fluorescent probes for sensor readout and manganese dioxide nanoflowers (MnO2 NFs) and as QDs nanocarriers for signal amplification. Prior to testing, amino-modified MnO2 nanoflowers (MnO2-NH2 NFs) were conjugated with carboxyl-modified QDs through EDC/NHSS method to form MnO2-QD NFs, and MnO2-QD NFs were functionalized with polyclonal antibodies (pAbs) to form MnO2-QD-pAb NFs. First, the mixture of target Salmonella Typhimurium cells and magnetic nanoparticles (MNPs) modified with monoclonal antibodies (mAbs) was injected with MnO2-QD-pAb NFs into a microfluidic chip to form MNP-bacteria-QD-MnO2 complexes. Then, glutathione (GSH) was injected to dissolve MnO2 on the complexes into Mn2+, resulting in the release of QDs. Finally, fluorescent intensity of the released QDs was measured using the fluorescent detector to determine the amount of Salmonella. A linear relationship between fluorescent intensity and bacterial concentration from 1.0 × 102 to 1.0 × 107 CFU/mL was found with a low detection limit of 43 CFU/mL and mean recovery of 99.7% for Salmonella in spiked chicken meats, indicating the feasibility of this biosensor for practical applications.

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“…ELISA is fast, but lacks sufficient sensitivity and has relatively high false positives. In recent two years, various biosensors, such as optical 3 - 8 , electrochemical 9 - 14 , magnetic 15 - 16 , quartz crystal microbalance 17 - 18 , acoustic 19 - 20 and surface plasmon resonance 21 - 22 , etc., have been reported for the detection of E. coli O157:H7 and other bacteria. Most biosensors are still in lab research now, and have not been ready for practical use yet.…”

Section: Introductionmentioning

confidence: 99%

An Electrochemical Aptasensor Using Coaxial Capillary with Magnetic Nanoparticle, Urease Catalysis and PCB Electrode for Rapid and Sensitive Detection of Escherichia coli O157:H7

et al. 2017

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The continuous outbreaks of foodborne diseases have drawn public attentions to food safety. Early screening of foodborne pathogens is crucial to prevent and control of foodborne diseases. In this study, a novel electrochemical aptasensor was developed for rapid and sensitive detection of E. coli O157:H7 using the coaxial capillary with immune magnetic nanoparticles (MNPs) for specific separation of the target bacteria, the urease with urea for amplification of the impedance signals, and the PCB gold electrode for measurement of the impedance change. The streptavidin modified MNPs were conjugated with the biotinylated polyclonal antibodies (PAbs) to form the immune MNPs, and captured in the coaxial capillary with the line-up high gradient magnetic fields to separate the bacteria from the large volume of sample. Then, the gold nanoparticles (GNPs) were modified with the aptamers against E. coli and the urease, and injected into the capillary to react with the bacteria and form the MNP-PAb-bacteria-aptamer-GNP-urease complexes. Finally, the urease on the complexes was used to catalyze the hydrolysis of urea into ammonium ions and carbonate ions in the capillary, leading to the decrease in the impedance of the catalysate, which was measured by the gold plating PCB electrode. The impedance change of the catalysate and the concentration of the bacteria had a good linear relationship. This aptasensor was able to detect E. coli as low as 101 CFU/mL in 3 h, and the mean recovery of E. coli in the spiked pasteurized milk was ~99%. This proposed aptasensor has the potential for practical applications of foodborne pathogen detection due to its short detection time, high sensitivity and low cost.

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QCM-based aptamer selection and detection of Salmonella typhimurium

Cited by 126 publications

References 32 publications

Development of gold nanoparticle-aptamer-based LSPR sensing chips for the rapid detection of Salmonella typhimurium in pork meat

Development of gold nanoparticle-aptamer-based LSPR sensing chips for the rapid detection of Salmonella typhimurium in pork meat

A Microfluidic Biosensor Based on Magnetic Nanoparticle Separation, Quantum Dots Labeling and MnO2 Nanoflower Amplification for Rapid and Sensitive Detection of Salmonella Typhimurium

An Electrochemical Aptasensor Using Coaxial Capillary with Magnetic Nanoparticle, Urease Catalysis and PCB Electrode for Rapid and Sensitive Detection of Escherichia coli O157:H7

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