QCM DNA biosensor for the diagnosis of a fish pathogenic virus VHSV

Hong, Sung-Rok; Jeong, Hyun Do; Hong, Suhee

doi:10.1016/j.talanta.2010.04.065

Cited by 61 publications

(35 citation statements)

References 11 publications

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“…Hereby, some of the most promising results of DNA-QCM biosensors for different viruses are discussed. For instance, DNA-QCM biosensors have been successfully tested for the detection of hepatitis B virus (HepBV) [105][106][107], hepatitis C virus (HepCV) [117], vaccinia virus [108], viral haemorrhagic septicaemia virus (VHSV) [109], human papilloma virus (HPV) [110], dengue virus [118], and orchid viruses such as cymbidium mosaic virus (CymMV) and odontoglossum ringspot virus (ORSV) [111].…”

Section: Dna and Aptamers For Qcm Virus Sensorsmentioning

confidence: 99%

Gravimetric Viral Diagnostics: QCM Based Biosensors for Early Detection of Viruses

et al. 2017

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Viruses are pathogenic microorganisms that can inhabit and replicate in human bodies causing a number of widespread infectious diseases such as influenza, gastroenteritis, hepatitis, meningitis, pneumonia, acquired immune deficiency syndrome (AIDS) etc. A majority of these viral diseases are contagious and can spread from infected to healthy human beings. The most important step in the treatment of these contagious diseases and to prevent their unwanted spread is to timely detect the disease-causing viruses. Gravimetric viral diagnostics based on quartz crystal microbalance (QCM) transducers and natural or synthetic receptors are miniaturized sensing platforms that can selectively recognize and quantify harmful virus species. Herein, a review of the label-free QCM virus sensors for clinical diagnostics and point of care (POC) applications is presented with major emphasis on the nature and performance of different receptors ranging from the natural or synthetic antibodies to selective macromolecular materials such as DNA and aptamers. A performance comparison of different receptors is provided and their limitations are discussed.

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Section: Dna and Aptamers For Qcm Virus Sensorsmentioning

confidence: 99%

Gravimetric Viral Diagnostics: QCM Based Biosensors for Early Detection of Viruses

et al. 2017

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“…It has been widely used as a powerful analytical tool in medical diagnostics [1À3], environmental testing [4À6] and food analysis [7]. In principle, a biosensor is fabricated by attaching a bio-receptor such as DNA probes, enzymes, antibodies or cells on the surface of a suitable transducer, which can convert a biological response into an electrical [3,4,8], optical [2] or mechanical [1,7,9] signal. As previously mentioned [10], the signal processor plays an important role in the DNA sensor.…”

Section: Introductionmentioning

confidence: 99%

Novel portable electrical detection system for DNA SENSOR application

Tam

Hong

Hieu

2012

Journal of Experimental Nanoscience

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A novel, portable, electrical detection system was constructed for DNA sensor application to detect DNA hybridisation. The read-out circuit consists of an analogue circuit and a digital circuit. The analogue circuit with an IC MAX038 generates a sine wave with a constant frequency (10 kHz), which serves as the input for the DNA sensor. The DNA sensor consists of active and reference sensors. DNA hybridisation between the DNA probe and the target sequences causes changes in the conductance of the conductive membrane (DNA/MWCNTs) on the sensor surface, which lead to changes in the amplitude of the sine wave from the sensor output compared with that of the reference signal output (input sine wave). We used a digital circuit with a microprocessor (PIC33FJ256MC710) to determine the change in the amplitude of the sine wave signal of the sensor. From these digital data, the difference in the amplitudes of the active and reference sensors was calculated and displayed on the liquid crystal display. Measurement results show that the portable electrical detection system can detect DNA target concentrations as low as 0.16 mM. The detection of the amplified polymerase chain reaction sample and the reproducibility of the DNA sensor results were also determined using the designed readout circuit. The proposed electrical detection system is suitable for DNA sensor application.

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“…Target was detected using DNA probes immobilized onto a SAM layer. 164 The study of Hong et al 165 described a rapid and sensitive QCM biosensor for diagnosis of viral hemorrhagic septicemia infection in fish. The main viral RNA-encoding G protein was detected by the specific probe.…”

mentioning

confidence: 99%

“…The main viral RNA-encoding G protein was detected by the specific probe. 165 Three different probes and three different immobilization methods were employed. 165 The most efficient (avidin-biotin probe immobilization) was more sensitive and much faster than a conventional reverse transcription-PCR, with detection limit of 0.0016 µM.…”

mentioning

confidence: 99%

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Nanoscale virus biosensors: state of the art

Krejčová¹,

Michálek²,

Rodrigo³

et al. 2015

NDD

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Abstract:Since the beginning of the new millennium, viruses have shown huge epidemiological and pandemic potential: severe acute respiratory syndrome (SARS) in 2002, pandemic swine flu in 2009, and last but not least the West African Ebola outbreak in 2014. The occurrence and spread of the new virus in pandemic dimension poses a threat to the health and lives of 7 billion people worldwide. There is a growing urgency for highly sensitive and selective detection techniques, usable for a wide number of applications, including disease diagnosis, pharmaceutical research, agriculture, as well as preventive measures. Nanobiosensors represent a new promising tool for virus detection. This review gives a brief survey of the issue of viral detection, comprising diagnostics of target structure of viruses such as nucleic acids or proteins. This review covers different detection principles, methods of fabrication, and applications of virus biosensors.

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QCM DNA biosensor for the diagnosis of a fish pathogenic virus VHSV

Cited by 61 publications

References 11 publications

Gravimetric Viral Diagnostics: QCM Based Biosensors for Early Detection of Viruses

Gravimetric Viral Diagnostics: QCM Based Biosensors for Early Detection of Viruses

Novel portable electrical detection system for DNA SENSOR application

Nanoscale virus biosensors: state of the art

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