Qingchang Huashi Formula attenuates DSS-induced colitis in mice by restoring gut microbiota-metabolism homeostasis and goblet cell function

Hu, Jingyi; Huang, Hai; Che, Yuan; Ding, Chujie; Zhang, Lu; Wang, Yun; Hao, Haiping; Shen, Hong; Cao, Lijuan

doi:10.1016/j.jep.2020.113394

Cited by 91 publications

(42 citation statements)

References 22 publications

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“…Intestinal mucus, synthesised by goblet cells, forms a gel-like layer that fills the crypts and serves as a barrier to protect the intestinal epithelium from the deleterious effects of luminal stimulants. Disruption of goblet cells can lead to intestinal inflammation [36]. It has been reported that IBD patients have reduced numbers of goblet cells and mucus layer thickness [37].…”

Section: Discussionmentioning

confidence: 99%

Anti-Inflammatory Effects of Rosmarinic Acid-Loaded Nanovesicles in Acute Colitis through Modulation of NLRP3 Inflammasome

et al. 2021

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Ulcerative colitis (UC), one of the two main types of inflammatory bowel disease, has no effective treatment. Rosmarinic acid (RA) is a polyphenol that, when administered orally, is metabolised in the small intestine, compromising its beneficial effects. We used chitosan/nutriose-coated niosomes loaded with RA to protect RA from gastric degradation and target the colon and evaluated their effect on acute colitis induced by 4% dextran sodium sulphate (DSS) for seven days in mice. RA-loaded nanovesicles (5, 10 and 20 mg/kg) or free RA (20 mg/kg) were orally administered from three days prior to colitis induction and during days 1, 3, 5 and 7 of DSS administration. RA-loaded nanovesicles improved body weight loss and disease activity index as well as increased mucus production and decreased myeloperoxidase activity and TNF-α production. Moreover, RA-loaded nanovesicles downregulated protein expression of inflammasome components such as NLR family pyrin domain-containing 3 (NLRP3), adaptor protein (ASC) and caspase-1, and the consequent reduction of IL-1β levels. Furthermore, nuclear factor erythroid 2-related factor 2 (Nrf2) and heme oxygenase-1 (HO-1) protein expression increased after the RA-loaded nanovesicles treatment However, these mechanistic changes were not detected with the RA-free treatment. Our findings suggest that the use of chitosan/nutriose-coated niosomes to increase RA local bioavailability could be a promising nutraceutical strategy for oral colon-targeted UC therapy.

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Section: Discussionmentioning

confidence: 99%

Anti-Inflammatory Effects of Rosmarinic Acid-Loaded Nanovesicles in Acute Colitis through Modulation of NLRP3 Inflammasome

et al. 2021

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“…The DAI and histopathology were assessed in each group of mice. The DAI was determined by combining hematochezia, mucous stools, and body weight loss scores, and dividing them by 3 (21). Colon tissues were fixed in 4% paraformaldehyde fixation solution, dehydrated through an ethanol gradient, rendered transparent by immersion in xylene, and finally embedded in paraffin, from which 4 mm paraffin slices were sectioned and stained with hematoxylin and eosin (HE).…”

Section: Evaluation Of Colitismentioning

confidence: 99%

Kaempferol Alleviates Murine Experimental Colitis by Restoring Gut Microbiota and Inhibiting the LPS-TLR4-NF-κB Axis

Yan

et al. 2021

Front. Immunol.

174

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Intestinal microbiota dysbiosis is an established characteristic of ulcerative colitis (UC). Regulating the gut microbiota is an attractive alternative UC treatment strategy, considering the potential adverse effects of synthetic drugs used to treat UC. Kaempferol (Kae) is an anti-inflammatory and antioxidant flavonoid derived from a variety of medicinal plants. In this study, we determined the efficacy and mechanism of action of Kae as an anti-UC agent in dextran sulfate sodium (DSS)-induced colitis mice. DSS challenge in a mouse model of UC led to weight loss, diarrhea accompanied by mucous and blood, histological abnormalities, and shortening of the colon, all of which were significantly alleviated by pretreatment with Kae. In addition, intestinal permeability was shown to improve using fluorescein isothiocyanate (FITC)–dextran administration. DSS-induced destruction of the intestinal barrier was also significantly prevented by Kae administration via increases in the levels of ZO-1, occludin, and claudin-1. Furthermore, Kae pretreatment decreased the levels of IL-1β, IL-6, and TNF-α and downregulated transcription of an array of inflammatory signaling molecules, while it increased IL-10 mRNA expression. Notably, Kae reshaped the intestinal microbiome by elevating the Firmicutes to Bacteroidetes ratio; increasing the linear discriminant analysis scores of beneficial bacteria, such as Prevotellaceae and Ruminococcaceae; and reducing the richness of Proteobacteria in DSS-challenged mice. There was also an evident shift in the profile of fecal metabolites in the Kae treatment group. Serum LPS levels and downstream TLR4-NF-κB signaling were downregulated by Kae supplementation. Moreover, fecal microbiota transplantation from Kae-treated mice to the DSS-induced mice confirmed the effects of Kae on modulating the gut microbiota to alleviate UC. Therefore, Kae may exert protective effects against colitis mice through regulating the gut microbiota and TLR4-related signaling pathways. This study demonstrates the anti-UC effects of Kae and its potential therapeutic mechanisms, and offers novel insights into the prevention of inflammatory diseases using natural products.

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“…The microbial dysbiosis in UC patients includes the decreased diversity and microbiota composition disorder ( 231 ). Dà Huáng Mŭ Dān Tāng (Rhubarb and peony bark Decoction) ( 223 ), P í ng Wèi S ă n (Stomach-Calming Powder) ( 225 ), Qing Cháng Huà Shī Tāng (Intestine-Clearing and Dampness-Removing Decoction) ( 226 ), Sān Huáng Shú Ài Tāng ( 228 ) and Bā wèi Xī lèi Sǎn ( 232 ) all could restore the diversity of the intestinal microbiota in DSS-treated mice. Meanwhile, it was found that the intestinal bacteria in DSS-treated mice also showed a recovery after administration: the abundance of Firmicutes increased, the amount of Bacteroides and Proteobacteria decreased, and the amount of lactobacillus increased.…”

Section: The Medication Of Ucmentioning

confidence: 99%

“…The dysbiosis of intestinal bacteria will be secondary to the imbalance of the microbiota metabolites and products in the development of UC ( 5 ). Interestingly, Qīng Cháng Huà Shi Tāng restores the balance between BAs metabolism and SCFAs production ( 226 ).…”

Section: The Medication Of Ucmentioning

confidence: 99%

The Communication Between Intestinal Microbiota and Ulcerative Colitis: An Exploration of Pathogenesis, Animal Models, and Potential Therapeutic Strategies

et al. 2021

Front. Med.

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Ulcerative Colitis (UC) is a chronic inflammatory bowel disease. The prolonged course of UC and the lack of effective treatment management make it difficult to cure, affecting the health and life safety of patients. Although UC has received more attention, the etiology and pathogenesis of UC are still unclear. Therefore, it is urgent to establish an updated and comprehensive understanding of UC and explore effective treatment strategies. Notably, sufficient evidence shows that the intestinal microbiota plays an important role in the pathogenesis of UC, and the treating method aimed at improving the balance of the intestinal microbiota exhibits a therapeutic potential for UC. This article reviews the relationship between the genetic, immunological and microbial risk factors with UC. At the same time, the UC animal models related to intestinal microbiota dysbiosis induced by chemical drugs were evaluated. Finally, the potential value of the therapeutic strategies for restoring intestinal microbial homeostasis and treating UC were also investigated. Comprehensively, this study may help to carry out preclinical research, treatment theory and methods, and health management strategy of UC, and provide some theoretical basis for TCM in the treatment of UC.

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Qingchang Huashi Formula attenuates DSS-induced colitis in mice by restoring gut microbiota-metabolism homeostasis and goblet cell function

Cited by 91 publications

References 22 publications

Anti-Inflammatory Effects of Rosmarinic Acid-Loaded Nanovesicles in Acute Colitis through Modulation of NLRP3 Inflammasome

Anti-Inflammatory Effects of Rosmarinic Acid-Loaded Nanovesicles in Acute Colitis through Modulation of NLRP3 Inflammasome

Kaempferol Alleviates Murine Experimental Colitis by Restoring Gut Microbiota and Inhibiting the LPS-TLR4-NF-κB Axis

The Communication Between Intestinal Microbiota and Ulcerative Colitis: An Exploration of Pathogenesis, Animal Models, and Potential Therapeutic Strategies

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