Qingchangligan formula alleviates acute liver injury by attenuating extracellular histone-associated inflammation

Liu, Ding; Zhang, Xiangying; Li, Li; Gou, Chunyan; Luo, Xiaolan; Yang, Yang; Wen, Tao; Li, Xiuhui

doi:10.1016/j.biopha.2018.01.121

Cited by 8 publications

(4 citation statements)

References 20 publications

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“…An ALF model has been well established [ 14 , 25 ]. From the perspective of TCM, “stasis” and “phlegm” define the key pathogenic features of ALF [ 26 ]. We used Xfd, which is a prescription that has been applied to treat ALF.…”

Section: Discussionmentioning

confidence: 99%

The Protective Effects of a Modified Xiaohua Funing Decoction against Acute Liver Failure in Mice Induced by D-Gal and LPS

Zhao

Liu

Xin

et al. 2022

Evidence-Based Complementary and Alternative Medicine

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Objective. The aim of this study was to evaluate the effects of a modified Xiaohua Funing decoction (Xfd) on acute liver failure (ALF) and determine whether the protective mechanisms are related to alterations in the gut microbiota. Methods. An animal model of ALF was induced by intraperitoneal injection of D-galactosamine (D-Gal, 0.5 g/kg) and lipopolysaccharide (LPS, 100 μg/kg). Male BALB/c mice were randomly divided into the following 4 groups: the control group (saline, Con), model group (D-Gal/LPS, Mod), silymarin pretreatment group (200 mg/kg, Sil), and modified Xfd pretreatment group (650 mg/kg, Xfd). The Sil and Xfd groups received the respective intervention orally for 14 days and 2 h before D-Gal/LPS treatment. The liver injury markers included alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels and liver histology. 16S rRNA gene sequencing was performed to assess the effects on the caecum content. Results. D-Gal/LPS treatment caused severe ALF, illustrating that the ALF model was successfully established. The administration of Sil and Xfd greatly reduced the serum ALT and AST levels and improved the pathological signs of liver injury. However, no significant difference was found between the two groups. In contrast to the Mod group, the Sil and Xfd groups showed a shift toward the Con group in terms of the gut microbiota structure. The abundances of Firmicutes and Bacteroidetes and the Bacteroidetes/Firmicutes ratio in the Mod group significantly differed from those in the Con group. The Sil and Xfd groups showed restoration of the disordered microbiota. Significantly increased relative abundances of Lachnospiraceae_NK4A136_group and Candidatus_Saccharimonas and a markedly decreased Muribaculaceae abundance were found in the Sil and Xfd mice compared with those in the Mod mice ( P < 0.01 , P < 0.05 ). Interestingly, a negative correlation was observed between the abundances of the gut microbiota constituents, specifically Clostridia_UCG-014, and ALT and AST levels. Conclusion. In summary, our results indicate that Xfd may protect the liver and modify the gut microbiota in ALF mice.

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Section: Discussionmentioning

confidence: 99%

The Protective Effects of a Modified Xiaohua Funing Decoction against Acute Liver Failure in Mice Induced by D-Gal and LPS

Zhao

Liu

Xin

et al. 2022

Evidence-Based Complementary and Alternative Medicine

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“…Together, circulating histones macroH2A1.2, H2B, and H4 were associated with MAFLD disease severity, further suggesting potential prognostic and patient stratification properties (Table 4). 77,[240][241][242][243][244][245][246][247][248][249][250] Interestingly, pediatric NAFLD patients were characterized by contrasting histone expression signature in circulation. Specifically, serum levels of macroH2A1.2 in NAFLD children were significantly elevated compared with healthy controls.…”

Section: Circulating Histones As Markers and Predictors Of Nafld/nashmentioning

confidence: 99%

Liquid Liver Biopsy for Disease Diagnosis and Prognosis

Tsoneva¹,

Ivanov²,

Vinciguerra³

2023

J Clin Transl Hepatol

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Liver diseases are a major burden worldwide, the scope of which is expected to further grow in the upcoming years. Clinically relevant liver dysfunction-related blood markers such as alanine aminotransferase and aspartate aminotransferase have limited accuracy. Nowadays, liver biopsy remains the gold standard for several liver-related pathologies, posing a risk of complication due to its invasive nature. Liquid biopsy is a minimally invasive approach, which has shown substantial potential in the diagnosis, prognosis, and monitoring of liver diseases by detecting disease-associated particles such as proteins and RNA molecules in biological fluids. Histones are the core components of the nucleosomes, regulating essential cellular processes, including gene expression and DNA repair. Following cell death or activation of immune cells, histones are released in the extracellular space and can be detected in circulation. Histones are stable in circulation, have a long half-life, and retain their post-translational modifications. Here, we provide an overview of the current research on histone-mediated liquid biopsy methods for liver diseases, with a focus on the most common detection methods.

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“…In one study, extracellular histones and proinflammatory cytokines in ACLF patients (conventional drugs + QCLGF) were lower than those in the conventional drug treatment group. In vivo experiments have revealed that QCLGF significantly improves the survival rate of concanavalin A-induced liver fibrosis mice, improves hepatotoxicity, and reduces extracellular histone levels and proinflammatory reactions ( 55 ).…”

Section: Types and Functions Of Damps Related To Aclfmentioning

confidence: 99%

The Immune Pathogenesis of Acute-On-Chronic Liver Failure and the Danger Hypothesis

Qiang

Liu

2022

Front. Immunol.

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Acute-on-chronic liver failure (ACLF) is a group of clinical syndromes related to severe acute liver function impairment and multiple-organ failure caused by various acute triggering factors on the basis of chronic liver disease. Due to its severe condition, rapid progression, and high mortality, it has received increasing attention. Recent studies have shown that the pathogenesis of ACLF mainly includes direct injury and immune injury. In immune injury, cytotoxic T lymphocytes (CTLs), dendritic cells (DCs), and CD4+ T cells accumulate in the liver tissue, secrete a variety of proinflammatory cytokines and chemokines, and recruit more immune cells to the liver, resulting in immune damage to the liver tissue, massive hepatocyte necrosis, and liver failure, but the key molecules and signaling pathways remain unclear. The “danger hypothesis” holds that in addition to the need for antigens, damage-associated molecular patterns (DAMPs) also play a very important role in the occurrence of the immune response, and this hypothesis is related to the pathogenesis of ACLF. Here, the research status and development trend of ACLF, as well as the mechanism of action and research progress on various DAMPs in ACLF, are summarized to identify biomarkers that can predict the occurrence and development of diseases or the prognosis of patients at an early stage.

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Qingchangligan formula alleviates acute liver injury by attenuating extracellular histone-associated inflammation

Cited by 8 publications

References 20 publications

The Protective Effects of a Modified Xiaohua Funing Decoction against Acute Liver Failure in Mice Induced by D-Gal and LPS

The Protective Effects of a Modified Xiaohua Funing Decoction against Acute Liver Failure in Mice Induced by D-Gal and LPS

Liquid Liver Biopsy for Disease Diagnosis and Prognosis

The Immune Pathogenesis of Acute-On-Chronic Liver Failure and the Danger Hypothesis

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