Qinzhuliangxue mixture alleviates psoriasis-like skin lesions via inhibiting the IL6/STAT3 axis

Qu, Keshen; Luo, Ying; Yan, Xiaoning; Kuai, Le; Ru, Yi; Luo, Yue; Song, Jiankun; Ji, Wanli; Li, Bin; Xing, Meng

doi:10.1016/j.jep.2021.114041

Cited by 11 publications

(4 citation statements)

References 31 publications

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“…Additionally, osthole, a principal component of Cnidium monnieri (L.) Cusson, exerted inhibitory effects on hypoxic HCT116 cells that may be associated with eukaryotic initiation factor 2 alpha phosphorylation-mediated apoptosis and the translational repression of hypoxia-inducible factor-1 (HIF-1) (Peng and Chou, 2022). We previously confirmed that HIF-1α was highly expressed in psoriatic lesions and could be affected by pathways regulated by TCM (Qu et al, 2021). Collectively, we chose caffeic acid, baicalin, obakunone, fraxinellone, and osthole to preliminarily establish quality control for BZLF (Figure 1).…”

Section: Quality Control Of Bzlfmentioning

confidence: 80%

Banzhilian formula alleviates psoriasis-like lesions via the LCN2/MMP-9 axis based on transcriptome analysis

Xing

Yan²,

Guo³

et al. 2023

Front. Pharmacol.

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Introduction: Oral Banzhilian formula (BZLF) is effective in the clinical treatment of psoriasis. However, the effectiveness and mechanism of different drug delivery routes deserve further study.Methods: First, we established the mouse model of psoriasis using imiquimod (IMQ), and high-performance liquid chromatography (HPLC) was used for the quality control of BZLF. Secondly, Total RNA Sequencing and bioinformatics analysis were used to explore the regulatory mechanism of BZLF in improving psoriatic lesions. Finally, further verification was based on animal experiments.Results: we externally applied BZLF for skin lesions in an imiquimod-induced psoriasis mouse model and found that BZLF alleviated psoriasis-like skin lesions while inhibiting the expression of Ki67 and inflammatory factors (Il17a, Tnf-α, S100a7 and Cxcl1) in skin lesions. Transcriptome sequencing results suggested that BZLF inhibited signalling pathways closely related to psoriatic inflammation, such as the IL-17 signalling pathway, chemokine signalling pathway, TNF signalling pathway, and NF-kappa B signalling pathway, and the protein-protein interaction (PPI) network identified LCN2 as one of the core target genes and screened out its regulated downstream gene MMP9.Discussion: Our findings suggest that the anti-psoriatic mechanism of BZLF involved in downregulating the LCN2/MMP-9 axis.

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Section: Quality Control Of Bzlfmentioning

confidence: 80%

Banzhilian formula alleviates psoriasis-like lesions via the LCN2/MMP-9 axis based on transcriptome analysis

Xing

Yan²,

Guo³

et al. 2023

Front. Pharmacol.

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“…The content of active ingredients in YJP was detected by HPLC, which is not restricted by the volatility and thermal stability of the samples, especially the compound with a high boiling point, macromolecular, strong polarity and poor heat stability. It is widely used in the analysis and detection of drug components due to the advantages of high sensitivity, fast analysis and high separation efficiency [40][41][42][43][44].…”

Section: Discussionmentioning

confidence: 99%

Optimization of Ethanol Extraction Technology for Yujin Powder Using Response Surface Methodology with a Box–Behnken Design Based on Analytic Hierarchy Process–Criteria Importance through Intercriteria Correlation Weight Analysis and Its Safety Evaluation

Jiang,

Zhang,

Zhao

et al. 2023

Molecules

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Here, we aimed to optimize the ethanol extraction technology for Yujin powder (YJP) and evaluate its safety. The ultrasonic-assisted ethanol reflux extraction method refluxing was used to extract YJP. The parameters were optimized through a combination of single-factor and response surface methodology (RSM). The comprehensive Y value score calculated using the content of 13 active ingredients in YJP ethanolic extracts (YEEs) and the yield of the dry extract were used as measuring criteria. RSM with a Box–Behnken design using three factors and three levels was adopted to optimize the ethanol extraction technology for YJP. Finally, acute and subchronic toxicity tests were performed to evaluate its safety. The results revealed the best technological parameters: a liquid–material ratio of 24:1, an ethanol concentration of 69%, assistance of ultrasound (40 °C, 50 kHZ, 30 min), reflux time of 53 min, and reflux temperature of 50 °C. In acute toxicity tests, the maximum administration dosage in mice was 28.21 g/kg, which is higher than 10 times the clinical dosage. Adverse effects in the acute and subchronic toxicity tests were not observed. All clinical indexes were normal. In conclusion, the RSM based on AHP–CRITIC weight analysis could be used to optimize the ethanol extraction technology for YJP and YEEs prepared under the above conditions and ensure high safety.

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“…Hsc70 and circOAS3 regulated phenotypes via JNK/STAT3/NF-κB signaling pathways Hsc70 was proved the involvement in the JNK/NF-κB signaling pathways [23]. STAT3, as well as IL-6, has a close relationship with JNK/NF-κB and they were all identi ed as playing crucial roles in in ammation reactions [24][25][26][27]. Based on the direct binding with each other, we speculated that circOAS3 and Hsc70 could involve in JNK/STAT3/NF-κB pathways in psoriatic keratinocytes.…”

Section: Hsc70 Regulates the Proliferation And Apoptosis Of Keratinoc...mentioning

confidence: 99%

CircOAS3 Regulates Keratinocyte Proliferation and Psoriatic Inflammation by Interacting with Hsc70 via the JNK/STAT3/NF-κB Signaling Pathway

et al. 2022

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Psoriasis is a chronic in ammatory disease of the skin with highly complex pathogenesis. CircRNAs play an important regulatory role in plenty of diseases as well as psoriasis. In this study, we identi ed that circOAS3 was signi cantly upregulated in both psoriatic tissues and M5-induced keratinocytes. Silencing circOAS3 in HaCaT and Ker-CT cells inhibited cell viability, promoted apoptosis, and blocked the cell cycle from G1 to S phase. RNA-pulldown and RNA immunoprecipitation (RIP) analysis identi ed the direct combination between circOAS3 and Heat shock cognate protein 70 (Hsc70). Silencing circOAS3 negatively in uences the Hsc70 in protein level rather than mRNA level. In mechanism, circOAS3 knockdown suppressed the activation of JNK/STAT3/NF-κB signaling pathways and blocked the nuclear accumulation of Hsc70 as well as phosphorylated STAT3/JNK/NF-κB as a result. Phenotypically, circOAS3 or Hsc70 silencing down-regulated the protein levels of IL-6 thus reducing psoriatic in ammation in vitro. In conclusion, interaction between circOAS3 and Hsc70 modulates the proliferation and psoriatic in ammation of HaCaT and Ker-CT cells through the JNK/STAT3/NF-κB signaling pathways, suggesting that circOAS3 or Hsc70 may be a promising therapeutic target for psoriasis. stem cells, including the expression of keratin 5 and p63. When grown in 3D organotypic culture, the epidermis formed by Ker-CT cells is pretty similar to that formed by primary keratinocytes under the same conditions [15]. Research showed Ker-CT cells are well suitable for studies on epidermal cell adhesion and Pemphigus pathomechanisms [17]. Here we make an attempt to use Ker-CT cells as a model of psoriasis as well as HaCaT cells.In this study, we determined circOAS3 was signi cantly up-regulated in psoriatic tissues and psoriatic cell lines. We further found that Hsc70 may function as the RNA binding protein (RBP) of circOAS3 to promote disease progression via JNK/STAT3/NF-κB signaling pathways. Therefore, circOAS3 or Hsc70 can serve as a biomarker for prognosis prediction and as a potential therapeutic target in treatment. Methods Patients and tissue samples collectionA total of 7 psoriatic samples were collected from patients with vulgaris psoriasis from Qilu Hospital of Shandong University. The patient did not receive systemic and local treatment within 3 months. All of them had typical psoriasis vulgaris clinical characteristics. 10 normal tissues were collected from healthy volunteers. Healthy subjects had no family history of psoriasis or any other autoimmune diseases.This study was approved by the Ethics Committee of Shandong University, Qilu Hospital (Jinan, China) and written informed consent was obtained from all patients. circRNAs microarray Affymetrix GeneChip Human Gene 2.0 ST Array (Invitrogen) was used for circRNAs expression pro ling. Cells were cryopulverized and homogenized using the Biopulverizer™ (Biospec) and Mini-Bead-Beater16 (Biospec), respectively. The homogenized samples were separated. RNA was precipitated, washed with 75% etha...

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Qinzhuliangxue mixture alleviates psoriasis-like skin lesions via inhibiting the IL6/STAT3 axis

Cited by 11 publications

References 31 publications

Banzhilian formula alleviates psoriasis-like lesions via the LCN2/MMP-9 axis based on transcriptome analysis

Banzhilian formula alleviates psoriasis-like lesions via the LCN2/MMP-9 axis based on transcriptome analysis

Optimization of Ethanol Extraction Technology for Yujin Powder Using Response Surface Methodology with a Box–Behnken Design Based on Analytic Hierarchy Process–Criteria Importance through Intercriteria Correlation Weight Analysis and Its Safety Evaluation

CircOAS3 Regulates Keratinocyte Proliferation and Psoriatic Inflammation by Interacting with Hsc70 via the JNK/STAT3/NF-κB Signaling Pathway

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