qPCR-Based Monitoring of 2-Methylisoborneol/Geosmin-Producing Cyanobacteria in Drinking Water Reservoirs in South Korea

Lee, Jung Eun; Park, Rumi; Yu, Mina; Byeon, Myeongseop; Kang, Taegu

doi:10.3390/microorganisms11092332

Cited by 3 publications

(2 citation statements)

References 42 publications

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“…A positive correlation between the copy number of the synthesis gene and T/O compounds has been identified in various studies, including those conducted in Paldang Lake in Korea [84], Qingcaosha Reservoir [61], and Lushui Reservoir in China [59]. Consequently, Cao et al (2023) [62] proposed that the abundance of the MIB synthesis gene (mic) in both the DNA and RNA forms could serve as valuable parameters for early warning systems associated with MIB production.…”

Section: The Concentrations Of Gsm In Various Water Bodies Exhibit Di...mentioning

confidence: 99%

Temporal Dynamics and Influential Factors of Taste and Odor Compounds in the Eastern Drinking Water Source of Chaohu Lake, China: A Comparative Analysis of Global Freshwaters

Shang,

Ke,

et al. 2024

Toxins

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The escalating proliferation of cyanobacteria poses significant taste and odor (T/O) challenges, impacting freshwater ecosystems, public health, and water treatment costs. We examined monthly variations in four T/O compounds from September 2011 to August 2012 in Chaohu Lake’s eastern drinking water source (DECL). More importantly, we compared the reported T/O occurrence and the related factors in freshwater bodies worldwide. The assessment of T/O issues indicated a severe and widespread problem, with many cases surpassing odor threshold values. Remarkably, China reported the highest frequency and severity of odor-related problems. A temporal analysis revealed variations in odor occurrences within the same water body across different years, emphasizing the need to consider high values in all seasons for water safety. Globally, T/O issues were widespread, demanding attention to variations within the same water body and across different layers. Algae were crucial contributors to odor compounds, necessitating targeted interventions due to diverse odorant sources and properties. A correlation analysis alone lacked definitive answers, emphasizing the essential role of further validation, such as algae isolation. Nutrients are likely to have influenced the T/O, as GSM and MIB correlated positively with nitrate and ammonia nitrogen in DECL, resulting in proposed control recommendations. This study offers recommendations for freshwater ecosystem management and serves as a foundation for future research and management strategies to address T/O challenges.

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Section: The Concentrations Of Gsm In Various Water Bodies Exhibit Di...mentioning

confidence: 99%

Temporal Dynamics and Influential Factors of Taste and Odor Compounds in the Eastern Drinking Water Source of Chaohu Lake, China: A Comparative Analysis of Global Freshwaters

Shang,

Ke,

et al. 2024

Toxins

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show abstract

“…However, in recent years, a wide range of fluctuations in temperature and rainfall, possibly linked to climate change, have complex effects on their occurrence. High concentrations of geosmin were detected up to a maximum of 1640 ngL −1 in the Paldang reservoir, used as a water supply source for over 20 million people, in November and December 2011 [12,13]. High concentrations of taste and odor compounds occurred in six water treatment plants around the reservoir [12,14].…”

Section: Introductionmentioning

confidence: 99%

Managing the Taste and Odor Compound 2-MIB in a River-Reservoir System, South Korea

Kang,

Kim,

Park

et al. 2023

Water

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High concentrations of 2-methylisoborneol (2-MIB) were reported during winter in the Paldang reservoir and North Han River, South Korea. The causes of the unusual taste and odor problems in the regulated river-reservoir system were not understood; however, a short-term solution is to flush out 2-MIB-rich water to secure water sources for over 20 million people. Approximately 150 million tons of water was released from upstream dams for 12 days (late November to early December 2018) to reduce the elevated levels of 2-MIB. Simultaneously, the spatio-temporal variations of the measured concentration of sample 2-MIB from five sites were simulated using a multi-dimensional hydrodynamics-based solute transport model to monitor the flushing effect. A modified environmental fluid dynamics code (EFDC) was adopted as the primary model framework. Five scenarios on the kinetic constants related to the characteristics of 2-MIB transport and behavior, such as conservative, net decay, and net production, were applied, and the results were compared. We found that the simulation errors on the elapsed times to satisfy the Korean drinking water monitoring standard (≤20 ngL−1) were smallest with the conservative dye transport option, indicating that the physical and biochemical characteristics of 2-MIB may not play an essential role.

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Effect of Culture Temperature on 2-Methylisoborneol Production and Gene Expression in Two Strains of Pseudanabaena sp.

Park,

Yu,

Park

et al. 2024

Cells

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The presence of the odorant 2-methylisoborneol (2-MIB) in drinking water sources is undesirable. Although 2-MIB production is known to be influenced by temperature, its regulation at the gene level and its relationship with Chlorophyll-a (Chl-a) at different temperatures remain unclear. This study investigates the impact of temperature on 2-MIB production and related gene expression in Pseudanabaena strains PD34 and PD35 isolated from Lake Paldang, South Korea. The strains were cultured at three temperatures (15, 25, and 30 °C) to examine cell growth, 2-MIB production, and mic gene expression levels. 2-MIB production per cell increased with higher temperatures, whereas mic gene expression levels were higher at lower temperatures, indicating a complex regulatory mechanism involving post-transcriptional and enzyme kinetics factors. Additionally, the relationship between Chl-a and 2-MIB involved in metabolic competition was analyzed, suggesting that high temperatures appear to favor 2-MIB synthesis more than Chl-a synthesis. The distinct difference in the total amount of the two products and the proportion of 2-MIB between the two strains partially explains the variations in 2-MIB production. These findings highlight the significant effect of temperature on 2-MIB biosynthesis in Pseudanabaena and provide a valuable background for gene data-based approaches to manage issues regarding 2-MIB in aquatic environments.

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qPCR-Based Monitoring of 2-Methylisoborneol/Geosmin-Producing Cyanobacteria in Drinking Water Reservoirs in South Korea

Cited by 3 publications

References 42 publications

Temporal Dynamics and Influential Factors of Taste and Odor Compounds in the Eastern Drinking Water Source of Chaohu Lake, China: A Comparative Analysis of Global Freshwaters

Temporal Dynamics and Influential Factors of Taste and Odor Compounds in the Eastern Drinking Water Source of Chaohu Lake, China: A Comparative Analysis of Global Freshwaters

Managing the Taste and Odor Compound 2-MIB in a River-Reservoir System, South Korea

Effect of Culture Temperature on 2-Methylisoborneol Production and Gene Expression in Two Strains of Pseudanabaena sp.

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