QseC Mediates Osmotic Stress Resistance and Biofilm Formation in Haemophilus parasuis

He, Lvqin; Dai, Ke; Wen, Xintian; Ding, Lingqiang; Cao, Sanjie; Huang, Xiaobo; Wu, Rui; Zhao, Qin; Huang, Yong; Yan, Qigui; Ma, Xiaoping; Han, Xinfeng; Wen, Yiping

doi:10.3389/fmicb.2018.00212

Cited by 36 publications

(24 citation statements)

References 41 publications

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“…Bacteria must source sufficient iron from the host and maintain a dynamic balance for their growth. Iron plays a key role in the growth of H. parasuis, and low availability in a host is a primary pressure for invasion of pathogenic bacteria and is considered a signal that leads to significant changes in cell processes (Deslandes et al, 2007;He et al, 2018). In the present study, 100 µM BIP was added to fresh T/V/S medium to construct an iron-restricted environment to analyze the capability of HPS5 and crp to utilize iron.…”

Section: Discussionmentioning

confidence: 98%

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Effect of cAMP Receptor Protein Gene on Growth Characteristics and Stress Resistance of Haemophilus parasuis Serovar 5

Jiang

Cheng

Cao

et al. 2020

Front. Cell. Infect. Microbiol.

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Haemophilus parasuis (HPS), a member of the family Pasteurellaceae, is a common bacteria in the upper respiratory tract of pigs but under certain circumstances can cause serious systemic disease (Glasser's disease) characterized by severe infection of the upper respiratory tract, fibrinous polyserositis, polyarthritis, and meningitis. cAMP receptor protein (CRP) is among the most important global regulators, playing a vital role in adapting to environmental changes during the process of bacterial infection. In order to investigate the function of the crp gene in the growth characteristics of H. parasuis serovar 5 (HPS5) and its ability to overcome adverse environmental stresses, a crp mutant strain (crp) was constructed and verified. In this study, we found that the crp gene was involved in growth rate, biofilm formation, stress tolerance, serum resistance, and iron utilization. Compared with the wild type, both the growth rate of the crp mutant and its resistance to osmotic pressure decreased significantly. Similar phenomena were also found in biofilm formation and iron utilization. However, the resistance to heat shock and serum complement of the crp mutant were enhanced. This study aimed to reveal the function in growth characteristics and stress resistance of the crp gene in HPS5. Whether it relates to virulence requires additional in-depth research.

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Section: Discussionmentioning

confidence: 98%

“…The ability of wild-type and crp mutant strains to utilize iron was measured as previously described (He et al, 2018;Dong et al, 2019) with some modifications. Briefly, the two strains were cultured to log-phase replication, and the value of OD 600 of the cultures was adjusted to 0.4.…”

Section: Iron Utilization Assaymentioning

confidence: 99%

Effect of cAMP Receptor Protein Gene on Growth Characteristics and Stress Resistance of Haemophilus parasuis Serovar 5

Jiang

Cheng

Cao

et al. 2020

Front. Cell. Infect. Microbiol.

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“…AI-2 has either a positive or negative effect on biofilm formation in different bacteria. 3/ AI-3 mainly in Gram-positive systems typically use secreted oligopeptides [13,14] and two-component systems (TCS), which consist of membrane-bound sensor kinase receptors (QseC) and cytoplasmic transcription factors (QseB) which direct alterations in gene expression [2,15]. The QseC receptor responds to AI-3 but also to AI-2 and, additionally, to epinephrine/ norepinephrine from eukaryotic hosts.…”

Section: 12mentioning

confidence: 99%

Cell Communications among Microorganisms, Plants, and Animals: Origin, Evolution and Interplays

Combarnous

Nguyen

2020

Preprint

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Cellular communications play pivotal roles in multi-cellular species, but they do so also in uni-cellular species. Moreover, cells communicate with each other not only within the same individual but also with cells in other individuals belonging to the same or other species. These communications occur between two unicellular species, two multicellular species, or between unicellular and multicellular species. The molecular mechanisms involved exhibit diversity and specificity, but they share common basic features which allow common pathways of communication between different, and sometimes very different species. These interactions have been made possible by the high degree of conservation of the basic molecular mechanisms of interaction of many ligand-receptor pairs in evolutionary remote species. These inter-species cellular communications played crucial roles during Evolution and must have been positively selected, particularly when collectively beneficial in hostile environments. We think that communications between cells did not arise after their emergence but was part of the very nature of first cells. Synchronization of populations of non-living protocells through chemical communications may have been a mandatory step towards their emergence as populations of living cells and explain the large commonality of cell communication mechanisms among microorganisms, plants, and animals.

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“…The biofilm formation test was performed as previously reported with some modifications [29,30]. Briefly, 20 µL of overnight culture of the wild-type strain and its mutants were inoculated into 2 mL of fresh TSB broth containing NAD and inactivated serum with or without glucose in 6-well plates, and then incubated for 24 h or 72 h at 37 °C.…”

Section: Biofilm Formation Assaymentioning

confidence: 99%

The Glaesserella parasuis phosphoglucomutase is partially required for lipooligosaccharide synthesis

Feng

Chen

Wang

et al. 2020

Vet Res

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Lipooligosaccharides (LOSs) are virulence determinants of Glaesserella parasuis, a pathogen of the respiratory tract of pigs. We previously reported that disruption of the galU or galE gene in G. parasuis results in increased sensitivity to porcine serum, indicating that the galactose catabolism pathway is required for polysaccharide formation in G. parasuis. Here, we evaluated the role of the HAPS_0849 gene in LOS synthesis. The G. parasuis SC096 HAPS_0849 mutant produced a highly truncated LOS molecule, although a small fraction of intact LOS was still observed, and this mutant was found to be more sensitive to serum than the parental strain. HAPS_0849 was overexpressed and purified for biochemical assays, and this protein exhibited phosphoglucomutase (PGM) activity. Heterologous expression of a pgm gene from Escherichia coli in the HAPS_0849 mutant led to restoration of the wild-type LOS glycoform, further demonstrating the PGM function of HAPS_0849 in G. parasuis. The autoagglutination and biofilm formation ability of this strain were also investigated. Disruption of HAPS_0849 led to an increased tendency to autoagglutinate and form more biofilms, and these enhanced phenotypes were observed in the absence of glucose. In addition, LOSs from HAPS_0849, galU and lgtB mutants had similar truncated glycoforms, while LOSs from the galE and lex-1 mutants exhibited another type of defective LOS pattern. These findings imply that HAPS_0849 may function upstream of GalU in the generation of glucose 1-phosphate. In conclusion, our results preliminarily described the functions of HAPS_0849 in G. parasuis, and this gene was partially required for LOS synthesis.

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QseC Mediates Osmotic Stress Resistance and Biofilm Formation in Haemophilus parasuis

Cited by 36 publications

References 41 publications

Effect of cAMP Receptor Protein Gene on Growth Characteristics and Stress Resistance of Haemophilus parasuis Serovar 5

Effect of cAMP Receptor Protein Gene on Growth Characteristics and Stress Resistance of Haemophilus parasuis Serovar 5

Cell Communications among Microorganisms, Plants, and Animals: Origin, Evolution and Interplays

The Glaesserella parasuis phosphoglucomutase is partially required for lipooligosaccharide synthesis

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