QsMYB1 expression is modulated in response to heat and drought stresses and during plant recovery in Quercus suber

Plant MYB Transcription Factors: Their Role in Drought Response Mechanisms

“…Several MYB TFs are regulated through mechanisms involving alternative splicing [ 12 , 155 , 156 , 157 ]. Recently, the QsMYB1 gene identified in oak ( Quercus suber L.) has been associated to the drought response and to alternative splicing mechanisms [ 96 , 97 ]. QsMYB1 codes for an R2R3-type MYB TF and its expression is putatively mediated by an alternative splicing mechanism that originates two different transcripts ( QsMYB1.1 and QsMYB1.2 ), differing in the 5′-untranslated region, with the QsMYB1.2 variant retaining the first intron.…”

“…QsMYB1 codes for an R2R3-type MYB TF and its expression is putatively mediated by an alternative splicing mechanism that originates two different transcripts ( QsMYB1.1 and QsMYB1.2 ), differing in the 5′-untranslated region, with the QsMYB1.2 variant retaining the first intron. Expression analysis of QsMYB1 transcripts showed that both transcripts were differently regulated in cork tissues and organs and that their amount was differently regulated by abiotic stress conditions [ 96 , 97 ]. In particular, increasing temperatures led to a gradual down-regulation of QsMYB1 splicing variants with a stronger effect on QsMYB1.1 abundance, whereas under drought condition QsMYB1 transcripts were transiently up-regulated, and QsMYB1.2 variant showed the highest drought-dependent induction.…”

“…In particular, increasing temperatures led to a gradual down-regulation of QsMYB1 splicing variants with a stronger effect on QsMYB1.1 abundance, whereas under drought condition QsMYB1 transcripts were transiently up-regulated, and QsMYB1.2 variant showed the highest drought-dependent induction. Both these two abiotic stresses followed by recovery triggered changes on the expression profile of the two QsMYB1 splicing variants indicating that the QsMYB1 TF is modulated at the post-transcriptional level by heat and drought stresses, which affect mainly the regulation of the spliced transcript QsMYB1.1 and of the un-spliced variant QsMYB1.2 , respectively [ 97 ].…”

ChIP-Seq reveals that QsMYB1 directly targets genes involved in lignin and suberin biosynthesis pathways in cork oak (Quercus suber)

“…Moreover, QsMYB1 transcripts are more abundant in cork, a highly suberized tissue, than in wood, a lignified but non-suberized tissue. The authors have also found several cis -acting regulatory elements related to phenylpropanoid pathway in the QsMYB1 promotor region [ 29 ]. QsMYB1 expression was also shown to be modulated in response to heat and drought stress, which points to a function in the regulatory network of cork oak response to abiotic stress [ 29 ].…”

“…The authors have also found several cis -acting regulatory elements related to phenylpropanoid pathway in the QsMYB1 promotor region [ 29 ]. QsMYB1 expression was also shown to be modulated in response to heat and drought stress, which points to a function in the regulatory network of cork oak response to abiotic stress [ 29 ]. These findings led the authors to hypothesize that QsMYB1 may be regulating one or more metabolic pathways involved in cork formation, namely the biosynthesis of lignin and suberin which constitute the major biopolymers present in cork.…”