QTL Analysis for Resistance to Phytophthora Blight (Phytophthora capsici Leon.) Using an Intraspecific Doubled-Haploid Population of Capsicum annuum

Sugita, Toru; Yamaguchi, Kazunori; Kinoshita, T.; Yuji, Kenichi; Sugimura, Yukiyo; Nagata, Russell T.; Kawasaki, Shinji; Todoroki, Atsushi

doi:10.1270/jsbbs.56.137

Cited by 43 publications

(24 citation statements)

References 27 publications

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“…S19 was mapped to P9, $6 cM below TG263 and centromeric with respect to the position for the 1-kb band of A2 (Figure 6), demonstrating that Bs2 resides on P9 in a region of the pepper genome that is orthologous to the top arm of potato XII that includes the fractionated orthologs Rx and Gpa2. This is consistent with results from others who have located several PCR markers with similarity to Bs2 on this chromosome (Ogundiwin et al 2005;Sugita et al 2006;DjianCaporalino et al 2007).…”

Section: Resultssupporting

confidence: 93%

The Fractionated Orthology of Bs2 and Rx/Gpa2 Supports Shared Synteny of Disease Resistance in the Solanaceae

et al. 2009

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Comparative genomics provides a powerful tool for the identification of genes that encode traits shared between crop plants and model organisms. Pathogen resistance conferred by plant R genes of the nucleotide-binding-leucine-rich-repeat (NB-LRR) class is one such trait with great agricultural importance that occupies a critical position in understanding fundamental processes of pathogen detection and coevolution. The proposed rapid rearrangement of R genes in genome evolution would make comparative approaches tenuous. Here, we test the hypothesis that orthology is predictive of R-gene genomic location in the Solanaceae using the pepper R gene Bs2. Homologs of Bs2 were compared in terms of sequence and gene and protein architecture. Comparative mapping demonstrated that Bs2 shared macrosynteny with R genes that best fit criteria determined to be its orthologs. Analysis of the genomic sequence encompassing solanaceous R genes revealed the magnitude of transposon insertions and local duplications that resulted in the expansion of the Bs2 intron to 27 kb and the frequently detected duplications of the 59-end of R genes. However, these duplications did not impact protein expression or function in transient assays. Taken together, our results support a conservation of synteny for NB-LRR genes and further show that their distribution in the genome has been consistent with global rearrangements.

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Section: Resultssupporting

confidence: 93%

The Fractionated Orthology of Bs2 and Rx/Gpa2 Supports Shared Synteny of Disease Resistance in the Solanaceae

et al. 2009

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“…The P. capsici inoculum suspension was prepared according to the method described by Sugita et al (2006) and Ueeda et al (2006), with a minor modification. Strains of P. capsici maintained by NIVTS were grown on V8 juice medium in 90-mm Petri dishes.…”

Section: Evaluation Of Resistance To Phytophthora Blightmentioning

confidence: 99%

Evaluation of Resistance to Bacterial Wilt and Phytophthora Blight in Capsicum Genetic Resources Collected in Myanmar

Matsunaga

Takeo

Saito

2011

J. Japan. Soc. Hort. Sci.

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We evaluated 41 Capsicum accessions collected in Myanmar for resistance to bacterial wilt and Phytophthora blight. Eighteen accessions were highly resistant to bacterial wilt. They consisted of 11 C. annuum, 6 C. frutescens, and 1 C. chinense, and had a wide range of fruit sizes and shapes. Seventeen were moderately resistant. On the other hand, only 1 accession was highly resistant to Phytophthora blight, and all other accessions were susceptible. These results indicate the existence of a range of materials in Myanmar that are highly resistant to bacterial wilt, but few accessions that are resistant to Phytophthora blight. The accession that was highly resistant to Phytophthora blight was also classified as moderately resistant to bacterial wilt, and will therefore be a useful genetic resource in breeding to produce combined resistance to both diseases.

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“…Research efforts to find the genetic basis of resistance have determined that it can be polygenic, with quantitative trait loci (QTL) mapping to several chromosomes (Sugita et al 2006). However, efforts to generate resistant cultivars through breeding programs have not resulted in cultivars with consistent levels of resistance.…”

Section: Introductionmentioning

confidence: 99%

Ethylene, but not salicylic acid or methyl jasmonate, induces a resistance response against Phytophthora capsici in Habanero pepper

et al. 2011

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We sprayed defence-related plant growth regulators (salicylic acid, methyl jasmonate and ethephon) on one-month-old Habanero pepper seedlings cultivated in vitro. Twenty-four hours later, we inoculated the seedlings with a virulent strain of Phytophthora capsici and periodically evaluated the disease symptoms. At the concentrations used, neither salicylic acid nor methyl jasmonate generated a protective effect in the seedlings, which died less than 10 days post inoculation. However, the treatment with 5 mM ethephon delayed or prevented disease symptoms in 30% of the seedlings. Interestingly, blocking the ethylene receptor with a previous application of 300 μM silver nitrate impeded the protective effects of ethephon. This result demonstrated that the plant resistance response required the perception of ethylene. Analysis of transcript populations in ethephon-treated seedlings revealed a direct correlation between survival and the accumulation of PR1, a gene marker of the systemic acquired resistance (SAR). Although the ethephon treatment also modified transcript levels of the plant defensin PDF1.2, a marker of the induced systemic resistance (ISR), in this case the accumulation also occurred when the ethylene receptor was blocked, suggesting a non-specific effect. The ethephon treatment did not modify the expression of NPR1 (a key transcriptional regulator of plant defence). Interestingly, transgenic pepper seedlings overexpressing endogenous PR10 or esterase genes, which are induced by the ET treatment, completely resisted the infection, which corroborated the importance of these genes in the defence response. Our results suggest that ethylene induced a systemic defence response in susceptible seedlings, possibly in an NPR1-independent pathway.

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QTL Analysis for Resistance to Phytophthora Blight (Phytophthora capsici Leon.) Using an Intraspecific Doubled-Haploid Population of Capsicum annuum

Cited by 43 publications

References 27 publications

The Fractionated Orthology of Bs2 and Rx/Gpa2 Supports Shared Synteny of Disease Resistance in the Solanaceae

The Fractionated Orthology of Bs2 and Rx/Gpa2 Supports Shared Synteny of Disease Resistance in the Solanaceae

Evaluation of Resistance to Bacterial Wilt and Phytophthora Blight in Capsicum Genetic Resources Collected in Myanmar

Ethylene, but not salicylic acid or methyl jasmonate, induces a resistance response against Phytophthora capsici in Habanero pepper

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