QTL analysis of powdery mildew resistance in cucumber (Cucumis sativus L.)

Sakata, Yoshiteru; Kubo, Nakao; Morishita, Masami; Kitadani, E.; Sugiyama, M.; Hirai, Masashi

doi:10.1007/s00122-005-0193-y

Cited by 13 publications

(28 citation statements)

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“…Transferability of SSRs between cucumber and melon has previously been indicated by cross-amplification and reciprocal mapping using cucumber and melon SSRs (e.g., Fazio et al 2003b, Gonzalo et al 2005, Sakata et al 2006, Fukino et al 2008b. In this study, we found amplified and polymorphic SSR markers that were present in both cucumber and melon, demonstrating the high transferability of our C. sativus SSRs to C. melo.…”

Section: Assessment Of Ssr Amplification and Polymorphismsupporting

confidence: 72%

“…As has been the case in other cucurbit crops, molecular genetic technologies such as marker-assisted selection (MAS) will be highly desirable tools to support breeding, since cucumber cultivation generally requires much time, labor, space, and equipment. Recently, genetic markers have been successfully used to select morphological characteristics (Fan et al 2006) and disease resistance (Sakata et al 2006). The development of genetic markers associated with other economically important characteristics is eagerly anticipated by cucumber breeders.…”

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confidence: 99%

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Development of 101 novel SSR markers and construction of an SSR-based genetic linkage map in cucumber (<i>Cucumis sativus</i> L.)

et al. 2008

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Section: Assessment Of Ssr Amplification and Polymorphismsupporting

confidence: 72%

mentioning

confidence: 99%

Development of 101 novel SSR markers and construction of an SSR-based genetic linkage map in cucumber (<i>Cucumis sativus</i> L.)

et al. 2008

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“…Brassica simple sequence repeat (SSR) markers (Lowe et al 2004, Suwabe et al 2002, Choi et al 2007, Ling et al 2007, Kim et al 2009) and Brassica sequence-tagged site (STS) markers (Kim et al 2006, Saito et al 2006, Choi et al 2007 were also used for the genotyping. The PCR reactions, detection of polymorphisms and construction of a linkage map were performed as described previously (Sakata et al 2006). A map of F 2 plants grown in 2005 was constructed using SSRs and STSs.…”

Section: Linkage and Qtl Analysesmentioning

confidence: 99%

“…The logarithm of odds (LOD) thresholds for each QTL (α = 0.05) were estimated as 3.7 and 3.3 in the 2007 and 2005 populations, respectively, by 1000 permutations. The QTL-linked AFLP fragments were cloned and sequenced for conversion into STS markers as described previously (Sakata et al 2006). The nucleotide sequences of the cloned AFLP fragments have been deposited to the DDBJ/EMBL/GenBank databases under accession nos.…”

Section: Linkage and Qtl Analysesmentioning

confidence: 99%

Detection of quantitative trait loci controlling morphological traits in Brassica rapa L.

et al. 2010

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The Brassica species displays great variation with regard to morphology. However, the genetic mechanisms that underlie this variability are mostly unknown. We generated 188 F 2 plants derived from a cross between a Chinese cabbage and a vegetable turnip, and scored several morphological traits (head formation, leaf lobe, pubescence, and turnip formation) twice (2005 and 2007) in two subsets of 94 individuals. With the exception of pubescence, we did not find any typical discrete segregation of these traits in F 2 plants, suggesting that they are controlled by multiple genes. We constructed a linkage map to conduct quantitative trait locus (QTL) analysis, and detected eight and 14 QTLs in 2007 and 2005, respectively, for head formation, leaf lobe, pubescence, turnip size, and turnip weight. The amplified fragment length polymorphism (AFLP) fragments linked to the QTLs were converted into sequence-tagged site (STS) markers. The QTLs detected here and their linkage markers may provide useful information for the selection of traits during the breeding of Chinese cabbage and turnip cultivars.

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“…Linkage analysis studies indicate that the DM and PM genes are located either at the same locus or in closely linked loci (Fanourakis, 1984). The first quantitative trait loci (QTLs) for PM resistance in cucumber were identified by Sakata et al (2006) who mapped 5 QTLs in four linkage groups on an unsaturated linkage map.…”

Section: Introductionmentioning

confidence: 99%

Genome-wide identification, characterization, and expression analysis of the MLO gene family in Cucumis sativus

Zhou¹,

Jing²,

Shi³

2013

Genet. Mol. Res.

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ABSTRACT. Mildew resistance locus o (MLO)is a plant-specific seven-transmembrane (TM) gene family. Several studies have revealed that certain members of the MLO gene family mediate powdery mildew susceptibility in three plant species, namely, Arabidopsis, barley, and tomato. The sequenced cucumber genome provides an opportunity to conduct a comprehensive overview of the MLO gene family. Fourteen genes (designated CsMLO01 through CsMLO14) have been identified within the Cucumis sativus genome by using an in silico cloning method with the MLO amino acid sequences of Arabidopsis thaliana and rice as probes. Sequence alignment revealed that numerous features of the gene family, such as TMs, a calmodulin-binding domain, peptide domains I and II, and 30 important amino acid residues for MLO function, are well conserved. Phylogenetic analysis of the MLO genes from cucumber and other plant species reveals seven different clades (I through VII). Three of these clades comprised MLO genes from A. thaliana, rice, maize, and cucumber, suggesting that these genes may have evolved after the divergence of monocots and dicots. In silico mapping showed that these CsMLOs were located on chromosomes 1, 2, 3, 4, 5, and 6 without any obvious clustering, except CsMLO01. To our knowledge, this paper is the first comprehensive report on MLO genes in C. sativus. These findings will facilitate the functional characterization of the MLOs related to powdery mildew susceptibility and assist in the development of disease resistance in cucumber.

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QTL analysis of powdery mildew resistance in cucumber (Cucumis sativus L.)

Abstract: The name of the fifth author, M. Sugiyama, was rendered incorrectly in the original publication.The online version of the original article can be found at http:// dx

Cited by 13 publications

References 0 publications

Development of 101 novel SSR markers and construction of an SSR-based genetic linkage map in cucumber (<i>Cucumis sativus</i> L.)

Development of 101 novel SSR markers and construction of an SSR-based genetic linkage map in cucumber (<i>Cucumis sativus</i> L.)

Detection of quantitative trait loci controlling morphological traits in Brassica rapa L.

Genome-wide identification, characterization, and expression analysis of the MLO gene family in Cucumis sativus

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