QTL analysis of powdery mildew resistance in cucumber (Cucumis sativus L.)

Sakata, Yoshiteru; Kubo, Nakao; Morishita, Masami; Kitadani, E.; Sugiyama, Masao; Hirai, Masashi

doi:10.1007/s00122-005-0121-1

Cited by 84 publications

(59 citation statements)

References 36 publications

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“…This result is consistent with the results from classical genetic analysis (Table S1). The seed color trait is relatively stable and is not affected by environmental factors [49]–[51]. In other crops, the number of genes controlling the seed coat color trait is variable; for example, the seed color trait is regulated by a single gene in flax [52], watermelon [53], and lettuce [54], while two independent loci were found in lentil [55] and biennial white sweet clover [56], and at least three genes are involved in controlling the trait in pea [57] and capsicum [58].…”

Section: Discussionmentioning

confidence: 99%

Genetic Analysis and QTL Mapping of Seed Coat Color in Sesame (Sesamum indicum L.)

et al. 2013

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Seed coat color is an important agronomic trait in sesame, as it is associated with seed biochemical properties, antioxidant content and activity and even disease resistance of sesame. Here, using a high-density linkage map, we analyzed genetic segregation and quantitative trait loci (QTL) for sesame seed coat color in six generations (P1, P2, F1, BC1, BC2 and F2). Results showed that two major genes with additive-dominant-epistatic effects and polygenes with additive-dominant-epistatic effects were responsible for controlling the seed coat color trait. Average heritability of the major genes in the BC1, BC2 and F2 populations was 89.30%, 24.00%, and 91.11% respectively, while the heritability of polygenes was low in the BC1 (5.43%), in BC2 (0.00%) and in F2 (0.89%) populations. A high-density map was constructed using 724 polymorphic markers. 653 SSR, AFLP and RSAMPL loci were anchored in 14 linkage groups (LG) spanning a total of 1,216.00 cM. The average length of each LG was 86.86 cM and the marker density was 1.86 cM per marker interval. Four QTLs for seed coat color, QTL1-1, QTL11-1, QTL11-2 and QTL13-1, whose heritability ranged from 59.33%–69.89%, were detected in F3 populations using CIM and MCIM methods. Alleles at all QTLs from the black-seeded parent tended to increase the seed coat color. Results from QTLs mapping and classical genetic analysis among the P1, P2, F1, BC1, BC2 and F2 populations were comparatively consistent. This first QTL analysis and high-density genetic linkage map for sesame provided a good foundation for further research on sesame genetics and molecular marker-assisted selection (MAS).

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Section: Discussionmentioning

confidence: 99%

Genetic Analysis and QTL Mapping of Seed Coat Color in Sesame (Sesamum indicum L.)

et al. 2013

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“…Quantitative trait loci (QTLs) associated with PM resistance have been mapped to six cucumber chromosomes [1, 2, 8, 11–13]. Although these findings have provided insights into the genetic control of cucumber PM resistance, the molecular defense mechanisms of host resistance against cucumber PM remain elusive.…”

Section: Introductionmentioning

confidence: 99%

Elucidation of the molecular responses of a cucumber segment substitution line carrying Pm5.1 and its recurrent parent triggered by powdery mildew by comparative transcriptome profiling

Shi

et al. 2017

BMC Genomics

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BackgroundPowdery mildew (PM) is one of the most severe fungal diseases of cucurbits, but the molecular mechanisms underlying PM resistance in cucumber remain elusive. In this study, we developed a PM resistant segment substitution line SSL508-28 that carried a segment on chromosome five representing the Pm5.1 locus from PM resistant donor Jin5-508 using marker-assisted backcrossing of an elite PM susceptible cucumber inbred line D8.ResultsWhole-genome resequencing of SSL508-28, Jin5-508 and D8 was performed to identify the exact boundaries of the breakpoints for this introgression because of the low density of available single sequence repeat markers. This led to the identification of a ~6.8 Mb substituted segment predicted to contain 856 genes. RNA-seq was used to study gene expression differences in PM treated (plants harvested 48 h after inoculation) and untreated (control) SSL508-28 and D8 lines. Exactly 1,248 and 1,325 differentially expressed genes (DEGs) were identified in SSL508-28 and D8, respectively. Of those, 88 DEGs were located in the ~6.8 Mb segment interval. Based on expression data and annotation, we identified 8 potential candidate genes that may participate in PM resistance afforded by Pm5.1, including two tandemly arrayed genes encoding receptor protein kinases, two transcription factors, two genes encoding remorin proteins, one gene encoding a P-type ATPase and one gene encoding a 70 kDa heat shock protein. The transcriptome data also revealed a complex regulatory network for Pm5.1-mediated PM resistance that may involve multiple signal regulators and transducers, cell wall modifications and the salicylic acid signaling pathway.ConclusionThese findings shed light on the cucumber PM defense mechanisms mediated by Pm5.1 and provided valuable information for the fine mapping of Pm5.1 and breeding of cucumber with enhanced resistance to PM.Electronic supplementary materialThe online version of this article (doi:10.1186/s12864-016-3438-z) contains supplementary material, which is available to authorized users.

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“…Some reports state that resistance is a quantitative trait controlled by multiple recessive genes (Smith, 1948;Barnes and Epps, 1956;Fugieda and Akiya, 1962;Kooistra, 1968;Kooistm, 1971;Zhang et al, 2005;Sakata et al, 2006). However, Shanmugasundarum et al (1971) differentiated between hypocotyl and leaf resistance and concluded that a recessive gene for hypocotyl resistance (pm-h) is essential for overall resistance.…”

Section: Introductionmentioning

confidence: 99%

“…In cucumber, there are various studies about the molecular biology of PM resistance. Sakata et al (2006) detected three quantitative trait loci (QTL) for PM resistance at 20°C and two at 26°C. Of these, one major QTL was found to confer resistance at both 20° and 26°C.…”

Section: Introductionmentioning

confidence: 99%

Research Article Molecular mapping and candidate gene analysis for resistance to powdery mildew in Cucumis sativus stem.

Liu

Han

et al. 2017

Genet. Mol. Res.

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ABSTRACT. Powdery mildew (PM) of cucumber (Cucumis sativus), caused by Podosphaera xanthii, is a major foliar disease worldwide and resistance is one of the main objectives in cucumber breeding programs. The resistance to PM in cucumber stem is important to the resistance for the whole plant. In this study, genetic analysis and gene mapping were implemented with cucumber inbred lines NCG-122 (with resistance to PM in the stem) and NCG-121 (with susceptibility in the stem). Genetic analysis showed that resistance to PM in the stem of NCG-122 was qualitative and controlled by a single-recessive nuclear gene (pm-s). Susceptibility was dominant to resistance. In the initial genetic mapping of the pm-s gene, 10 SSR markers were discovered to be linked to pm-s, which was mapped to chromosome 5 (Chr.5) of cucumber. The pm-s gene's closest flanking markers were SSR20486 and SSR06184/ SSR13237 with genetic distances of 0.9 and 1.8 cM, respectively. One hundred and fifty-seven pairs of new SSR primers were exploited by the sequence information in the initial mapping region of pm-s. The analysis on the F 2 mapping population using the new molecular markers showed that 17 SSR markers were confirmed to be linked to the pm-s gene. The two closest flanking markers, pmSSR27and pmSSR17, were 0.1 and 0.7 cM from pm-s, respectively, confirming the location of this gene on Chr.5. The physical length of the genomic region containing pm-s was 135.7 kb harboring 21 predicted genes. Among these genes, the gene Csa5G623470 annotated as encoding Mlo-related protein was defined as the most probable candidate gene for the pm-s. The results of this study will provide a basis for marker-assisted selection, and make the benefit for the cloning of the resistance gene.

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QTL analysis of powdery mildew resistance in cucumber (Cucumis sativus L.)

Cited by 84 publications

References 36 publications

Genetic Analysis and QTL Mapping of Seed Coat Color in Sesame (Sesamum indicum L.)

Genetic Analysis and QTL Mapping of Seed Coat Color in Sesame (Sesamum indicum L.)

Elucidation of the molecular responses of a cucumber segment substitution line carrying Pm5.1 and its recurrent parent triggered by powdery mildew by comparative transcriptome profiling

Research Article Molecular mapping and candidate gene analysis for resistance to powdery mildew in Cucumis sativus stem.

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