QTL analysis of seed-flooding tolerance in soybean (Glycine max [L.] Merr.)

Sayama, Takashi; Nakazaki, Tetsuya; Ishikawa, Goro; Yagasaki, Kazuhiro; Yamada, Naohiro; Hirota, Naoyoshi; Hirata, Keiichiro; Yoshikawa, Takanori; Saito, Hiroki; Teraishi, Masayoshi; Okumoto, Yutaka; Tsukiyama, Takuji; Tanisaka, Takatoshi

doi:10.1016/j.plantsci.2009.01.007

Cited by 81 publications

(64 citation statements)

References 20 publications

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“…The linkage map of the PT-RIL population reported by Sayama et al (2009) consists of 344 simple sequence repeat (SSR) markers and three conventional markers, Inhibitor (I), pubescence color (T) and flower color (W1) loci, covering 2652.5 cM. The average distance between markers was 7.6 cM.…”

Section: Qtl Analysis Of the Isoflavone Contentsmentioning

confidence: 99%

Transgressive segregation of isoflavone contents under the control of four QTLs in a cross between distantly related soybean varieties

et al. 2010

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Soybean (Glycine max (L) Merr.) isoflavones have attracted considerable attention for their diverse effects on human health. To determine the genetic factors that contribute to the high isoflavone contents of the soybean varieties 'Peking' and 'Tamahomare', we conducted QTL analyses for the total content of daidzein derivatives (DAC), genistein derivatives (GEC) and glycitein derivatives (GLC), and for the total content of isoflavones (TIC) using recombinant inbred lines (RILs) derived from the cross between 'Peking' and 'Tamahomare'. Ninety six RILs were planted in Kyoto, Osaka and Nagano in 2003 and in Osaka and Nagano in 2004. Transgressive segregation for TIC was detected in all the environments tested. Composite interval mapping for TIC revealed four QTLs: qIso1, qIso2, qIso3 and qIso4, located on LG-A1 (Chr.5), LG-A2 (Chr.8), LG-C1 (Chr.4) and LG-D2 (Chr.17), respectively. The high-isoflavone alleles were derived from Peking at qIso1 and qIso4 and from Tamahomare at qIso2 and qIso3. Several other groups have already reported the former two QTLs, but qIso2 and qIso3 are new discoveries. Our results indicate that the large variation in TIC measurements observed in the RILs could have resulted from the combined effects of alleles at the four QTLs derived from distantly related varieties.

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Section: Qtl Analysis Of the Isoflavone Contentsmentioning

confidence: 99%

Transgressive segregation of isoflavone contents under the control of four QTLs in a cross between distantly related soybean varieties

et al. 2010

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“…The straight growth of the soybean seedlings was in favor of recording unbiased data. Paper roll method did not used any germination and growth media as those of Sayama et al, (2009) and is also space efficient to screen large populations. The present study is first one to offer favorable and affordable testing procedure to examine the lasting effects of the seed-flooding stress up to 5th day of seedling growth and development.…”

Section: The Best Methods For Seed-flooding Tolerance Evaluationmentioning

confidence: 99%

“…While petri dish with two moist filter papers was adopted as the germination medium (Hou and Thseng, 1992). In study focusing the germplasm screening, 0.7% agar medium (500 ml) in a plastic tray with a clear cover (20 cm x 25 cm) under continuous light (25000 lx) at 25ºC was used by Sayama et al, (2009)…”

Section: Introductionmentioning

confidence: 99%

“…Previously the germination rate has been adopted as the flooding indicator by Hou and Thseng (1991), while Sayama et al, (2009) adopted normal seedling rate as the additional indicator to germination rate. The suppression to soybean emergence and reduction of seedling growth in terms of total dry weight was observed after 48 hours of seed soaking treatment (Nakayama et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

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Establishment of evaluation procedure for soybean seed-flooding tolerance and its application to screening for tolerant germplasm sources

Ali

Xing

et al. 2017

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The experiments concluded 48hrs for germination testing as most appropriate stress duration. Using paper roll for recording the traits was the best method than petri dish and towel method. Among the traits relative germination rate (RGR), relative seedling length (RSL), relative root length (RRL), relative root fresh weight (RRFW) and relative root dry weight (RRDW) were proved to be flooding-responsive. RSL was chosen as the major seed-flooding indicator due to higher heritability (h 2 ), genotypic coefficient of variation (GCV), correlation with other indicators and easier measuring procedure, while RRL was considered as the subsidiary indicator. Thus, the RSL in paper roll after 48hrs seed-flooding was recognized as standard seed-flooding testing procedure and used to evaluate breeding materials. Among the 11 cultivars, the superior seed-floodingtolerant ones were M8206, NN1138-2 and ZXD, while ten best lines were evaluated from one hundred breeding lines.

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“…Polymerase chain reaction (PCR) is the foundation of SSR molecular markers technology (Sayama et al, 2009;Liu et al, 2010); it relies on accurate amplification products. However, plant genomes are large and complex.…”

Section: Introductionmentioning

confidence: 99%

Single- and double-SSR primer combined analyses in rice

Guan²,

Zhang³

et al. 2012

Genet. Mol. Res.

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ABSTRACT. Polymerase chain reaction (PCR) is the foundation of SSR molecular marker technology. We used sib rice varieties J518, XD1 and SD23 as experimental materials, selecting 30 pairs of SSR primers, including RM127, RM337 and RM5172, covering the rice genome, and performed single-and double-SSR primer combined analyses. We found that under the same PCR system and conditions, a single primer of the SSR primer pairs could amplify the same fragments as double primers do. The sequencing results demonstrated that some amplified fragments that we previously believed to come from double primers were actually produced by a single primer. The use of this kind of primer, such as the RM127 primer pair, for marker-assisted breeding will therefore be misleading. Additionally, using the same PCR system and conditions, some single primers that are part of SSR primer pairs can amplify many more specific fragments than double-SSR primers. For instance, in the case of the RM5172 primer pair, a single primer P1 amplified approximately three times the number of fragments as the double primer. This information can contribute to research on genetic diversity of species, understanding of genetic relationships and identification of germplasm resources. Accordingly, combined analyses of single-and double-primer amplification products not only can remove single-primer amplification fragments and false-positives from Single-and double-SSR primer combined analyses in rice double-primer amplification products in order to improve test accuracy, but also can facilitate research on genetic diversity, exploration of phylogenetic relationships and identification of germplasm resources. We define this method as "single-and double-SSR primer combined analyses".

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QTL analysis of seed-flooding tolerance in soybean (Glycine max [L.] Merr.)

Cited by 81 publications

References 20 publications

Transgressive segregation of isoflavone contents under the control of four QTLs in a cross between distantly related soybean varieties

Transgressive segregation of isoflavone contents under the control of four QTLs in a cross between distantly related soybean varieties

Establishment of evaluation procedure for soybean seed-flooding tolerance and its application to screening for tolerant germplasm sources

Single- and double-SSR primer combined analyses in rice

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