QTL analysis of yield traits in an advanced backcross population derived from a cultivated Andean × wild common bean (Phaseolus vulgaris L.) cross

Abstract

Advanced backcross QTL analysis was used to identify quantitative trait loci (QTL) for agronomic performance in a population of BC2F(3:5) introgression lines created from the cross of a Colombian large red-seeded commercial cultivar, ICA Cerinza, and a wild common bean accession, G24404. A total of 157 lines were evaluated for phenological traits, plant architecture, seed weight, yield and yield components in replicated trials in three environments in Colombia and genotyped with microsatellite, SCAR, and phaseolin markers that were used to create a genetic map that covered all 11 linkage groups of the common bean genome with markers spaced at an average distance of every 10.4 cM. Segregation distortion was most significant in regions orthologous for a seed coat color locus (R-C) on linkage group b08 and two domestication syndrome genes, one on linkage group b01 at the determinacy (fin) locus and the other on linkage group b02 at the seed-shattering (st) locus. Composite interval mapping analysis identified a total of 41 significant QTL for the eight traits measured of which five for seed weight, two for days to flowering, and one for yield were consistent across two or more environments. QTL were located on every linkage group with b06 showing the greatest number of independent loci. A total of 13 QTL for plant height, yield and yield components along with a single QTL for seed size showed positive alleles from the wild parent while the remaining QTL showed positive alleles from the cultivated parent. Some QTL co-localized with regions that had previously been described to be important for these traits. Compensation was observed between greater pod and seed production and smaller seed size and may have resulted from QTL for these traits being linked or pleiotropic. Although wild beans have been used before to transfer biotic stress resistance traits, this study is the first to attempt to simultaneously obtain a higher yield potential from wild beans and to analyze this trait with single-copy markers. The wild accession was notable for being from a unique center of diversity and for contributing positive alleles for yield and other traits to the introgression lines showing the potential that advanced backcrossing has in common bean improvement.

Investigation of the domestication of common bean (Phaseolus vulgaris) using multilocus sequence data

“…Similar estimates have been observed in other crops (Morrell et al 2005;Caldwell et al 2006;Liu and Burke 2006;Zhu et al 2007). Wild common bean is divided into two gene pools (Singh et al 1991a(Singh et al , 1991bBlair et al 2006;Díaz and Blair 2006;McClean et al 2004;McClean and Lee 2007;Rossi et al 2009) that appear to have arisen from a common ancestor 110 000 years BP (S. Mamidi, M. Rossi, D. Annam, S. M. Moghaddam, R. K. Lee, R. Papa, P. E. McClean, unpubl. data).…”

Seleção fenotípica e assistida por marcadores moleculares de famílias de feijoeiro-comum com alta produtividade

“…Esses marcadores podem ser utilizados na seleção de genótipos superiores. Embora inúmeros QTL já tenham sido identifi cados para vários caracteres na maioria das culturas de grande importância, inclusive alguns para a produção de grãos em feijão-comum (Faleiro et al, 2003;Melo et al, 2004;Rodrigues, 2004;Teixeira, 2004;Blair et al, 2006b), são poucos os relatos de sucesso da incorporação dessas informações ao processo seletivo e na obtenção de novas cultivares (Yousef & Juvick, 2001;Moreau et al, 2004;Bernardo, 2008).…”

Identification of QTLs of resistance to white mold in common bean from multiple markers by using Bayesian analysis

“…However, Maxwell et al (2007) identified this marker in LG 9, evaluating the RIL G122/CO72548 population. This marker is also related to the QTLs for days to flowering, pods per plant, and 100 seed weight (Blair et al, 2006). In this study, the BM184 marker identified a QTL affecting increasing resistance to white mold at a distance of 3.34 cM from the QTL.…”

“…This same marker, according to Blair et al (2006), flanks QTLs for days to flowering, plant height, plant width, and number of seeds per plant and for the V gene for flower color. In this study, the BM187 marker identified the most significant QTL for the effect of reducing resistance to white mold.…”

“…The BM212 and ATA244 markers, despite being near the QTLs identified (at 2.36 and 1.87 cM, respectively), are not promising for MAS because they showed low heritability (0.02 and 1.01%, respectively). According to Blair et al (2006), the BM212 marker is also near a QTL for 100 seed weight.…”