QTL associated with gummy stem blight resistance in watermelon

Gimode, Winnie; Bao, Kan; Fei, Zhangjun; McGregor, Cecilia

doi:10.1007/s00122-020-03715-9

Cited by 28 publications

(43 citation statements)

References 58 publications

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“…ClGSB5.1) identi ed by Gimode et al ( 2021), the marker most closely associated to Qgsb5.1 (ClGSB5.1-1) was included in the map (Fig. 2c) 17 . ClGSB5.1-1 mapped 44.5 cM outside the con dence interval of the current QTL and it was therefore concluded that the QTL identi ed in the current study represents a novel QTL (Qgsb5.2).…”

Section: Qtl-seqmentioning

confidence: 99%

“…The species of Stagonosporopsis used by Lee et al (2021) is unknown. Gimode et al (2021) used a population derived from Crimson Sweet (C. lanatus) × PI 482276 (C. amarus) and S. citrulli isolate 12178A to map GSB resistance QTL on chromosomes 5 and 7 17 . Differences in the locations of these QTL, especially those derived from the same resistant parent (PI 189225), are likely due to either differences in resistance to the various species or isolates of Stagonosporopsis used for phenotyping or differences in phenotyping methodologies.…”

Section: Introductionmentioning

confidence: 99%

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Qtl Associated With Resistance to Stagonosporopsis Citrulli in Citrillus Amarus

Adams¹,

McGregor²

2022

Preprint

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Gummy stem blight (GSB) is a fungal disease affecting cucurbit crops, including watermelon (Citrullus lanatus), leading to significant yield losses. The disease is caused by three Stagonosporopsis species, of which S. citrulli is the most common in the southeastern United States. Currently no gummy stem blight-resistant watermelon cultivars are available to growers. In this study we used QTL-seq in an interspecific population developed from Sugar Baby × PI 189225 (C. amarus) to identify a novel QTL associated with resistance to S. citrulli (PVE = 13.3%) on chromosome 5 (Qgsb5.2) of the watermelon genome. KASP marker assays were developed for selection of Qgsb5.2 to allow breeders to track the allele contributing resistance to GSB, reducing the need for laborious phenotyping. Pyramiding different GSB resistance QTL could be a useful strategy to develop GSB resistant watermelon cultivars.

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Section: Qtl-seqmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Qtl Associated With Resistance to Stagonosporopsis Citrulli in Citrillus Amarus

Adams¹,

McGregor²

2022

Preprint

Self Cite

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“… Moumni et al (2020) reported that the selection of healthy fruit is not sufficient to reduce the infection by this seedborne pathogen. On the other hand, cultural practices and fungicide application can have important roles in GSB management, although this pathogen has developed resistance to many fungicides that were previously very effective ( Finger et al, 2014 ; Gimode et al, 2020 ; Newark et al, 2020 ). Therefore, early diagnosis of this pathogen is a fundamental step in the management of these crop diseases ( Ora et al, 2011 ).…”

Section: Introductionmentioning

confidence: 99%

Detection and Quantification of Stagonosporopsis cucurbitacearum in Seeds of Cucurbita maxima Using Droplet Digital Polymerase Chain Reaction

et al. 2022

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Stagonosporopsis cucurbitacearum is an important seedborne pathogen of squash (Cucurbita maxima). The aim of our work was to develop a rapid and sensitive diagnostic tool for detection and quantification of S. cucurbitacearum in squash seed samples, to be compared with blotter analysis, that is the current official seed test. In blotter analysis, 29 of 31 seed samples were identified as infected, with contamination from 1.5 to 65.4%. A new set of primers (DB1F/R) was validated in silico and in conventional, quantitative real-time PCR (qPCR) and droplet digital (dd) PCR. The limit of detection of S. cucurbitacearum DNA for conventional PCR was ∼1.82 × 10–2 ng, with 17 of 19 seed samples positive. The limit of detection for ddPCR was 3.6 × 10–3 ng, which corresponded to 0.2 copies/μl. Detection carried out with artificial samples revealed no interference in the absolute quantification when the seed samples were diluted to 20 ng. All seed samples that showed S. cucurbitacearum contamination in the blotter analysis were highly correlated with the absolute quantification of S. cucurbitacearum DNA (copies/μl) in ddPCR (R2 = 0.986; p ≤ 0.01). Our ddPCR protocol provided rapid detection and absolute quantification of S. cucurbitacearum, offering a useful support to the standard procedure.

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“…No commercial cultivars with resistance to gummy stem blight are available, and so this disease has been frequently managed by fungicides application. As a result, several fungicides have lost their effectiveness against this disease following the development of resistance by S. cucurbitacearum [28,31]. Therefore, the development of alternative strategies to the use of synthetic fungicides for seed treatments is essential for both integrated pest management and organic agriculture.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Seven Essential Oils as Seed Treatments against Seedborne Fungal Pathogens of Cucurbita maxima

et al. 2021

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Essential oils are gaining interest as environmentally friendly alternatives to synthetic fungicides for management of seedborne pathogens. Here, seven essential oils were initially tested in vivo for disinfection of squash seeds (Cucurbita maxima) naturally contaminated by Stagonosporopsis cucurbitacearum, Alternaria alternata, Fusarium fujikuro, Fusarium solani, Paramyrothecium roridum, Albifimbria verrucaria, Curvularia spicifera, and Rhizopus stolonifer. The seeds were treated with essential oils from Cymbopogon citratus, Lavandula dentata, Lavandula hybrida, Melaleuca alternifolia, Laurus nobilis, and Origanum majorana (#1 and #2). Incidence of S. cucurbitacearum was reduced, representing a range between 67.0% in L. nobilis to 84.4% in O. majorana #2. Treatments at 0.5 mg/mL essential oils did not affect seed germination, although radicles were shorter than controls, except with C. citratus and O. majorana #1 essential oils. Four days after seeding, seedling emergence was 20%, 30%, and 10% for control seeds and seeds treated with C. citratus essential oil (0.5 mg/mL) and fungicides (25 g/L difenoconazole plus 25 g/L fludioxonil). S. cucurbitacearum incidence was reduced by ~40% for plantlets from seeds treated with C. citratus essential oil. These data show the effectiveness of this essential oil to control the transmission of S. cucurbitacearum from seeds to plantlets, and thus define their potential use for seed decontamination in integrated pest management and organic agriculture.

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QTL associated with gummy stem blight resistance in watermelon

Cited by 28 publications

References 58 publications

Qtl Associated With Resistance to Stagonosporopsis Citrulli in Citrillus Amarus

Qtl Associated With Resistance to Stagonosporopsis Citrulli in Citrillus Amarus

Detection and Quantification of Stagonosporopsis cucurbitacearum in Seeds of Cucurbita maxima Using Droplet Digital Polymerase Chain Reaction

Evaluation of Seven Essential Oils as Seed Treatments against Seedborne Fungal Pathogens of Cucurbita maxima

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