QTL identification and microphenotype characterisation of the developmentally regulated yellow rust resistance in the UK wheat cultivar Guardian

Abstract

Yellow rust (causal agent: Puccinia striiformis f.sp. tritici) resistance in the UK wheat cultivar Guardian is developmentally regulated, resistance increasing as the plant matures. Yellow rust resistance was assessed under field conditions on plants after ear emergence to ensure maximum expression of resistance. Three quantitative trait loci (QTL) for yellow rust resistance were identified, being located on chromosomes 1B (QPst.jic-1B), 2D (QPst.jic-2D) and 4B (QPst.jic-4B). The largest resistance effect, QPst.jic-1B located to the same position on the long arm of chromosome 1B as the known durable source of yellow rust resistance, Yr29. Microscopic studies were carried out to determine what effect the resistance in Guardian had on the development of P. striiformis f.sp. tritici. While the adult plant resistance in Guardian did not prevent germinated urediniospores from establishing an effective infection site, the growth of hyphae within flag leaf tissue was significantly inhibited, slowing the development of microcolonies. 3,3-diaminabenzadine (DAB) and trypan blue staining indicated that this inhibition of hyphal growth was not associated with hydrogen peroxide accumulation or extensive plant cell death.

Mining Centuries Old In situ Conserved Turkish Wheat Landraces for Grain Yield and Stripe Rust Resistance Genes

“…In the current study, six potentially novel QTL for resistance to stripe rust were identified in combined and individual environment analyses (Table 2) and one additional candidate novel QTL was detected exclusively in club wheat (Supplementary Table 10). The novel genomic region identified on 2DS at 28.0 cM is approximately 90–100 cM away from previously reported QTL for stripe rust response on 2DS using bi-parental populations (Bariana et al, 2001; Suenaga et al, 2003; Mallard et al, 2005; Melichar et al, 2008; Lu et al, 2009; Agenbag et al, 2012). Naruoka et al (2015) recently reported a stable APR locus on 2DS (stable in nine environments) using GWA in a winter wheat association panel.…”

Mapping Stripe Rust Resistance in a BrundageXCoda Winter Wheat Recombinant Inbred Line Population

“…Several temporarily designated Yr genes have been mapped to 1B including: YrAlp [16] and YrC142 [54] on 1BS and YrCN17 [55], YrExp1 [17] and YrR212 [55] on 1BL. Furthermore several stripe rust resistance QTL have been mapped to chromosome 1B including: QYr.sun-1B [56], QPST.jic-1BL [57] and a QTL linked to Xgwm259 [58].…”

Review Article: High-Temperature Adult-Plant Resistance, Key for Sustainable Control of Stripe Rust

“…In addition, adultplant resistance to stripe rust controlled by a single gene or QTL was reported in wheat genotypes "Bersee" as Yr16 [61], in "Pavon F76" as Yr29 [62], in wheat genotype "Lgst. 79 -74" as Yrns-B1 [63], in "Opata 85" as Yr30 [64], in "Cook" [65], in a T. boeoticum transgressed wheat genotype as QYrtb.pau-5A and a T. monococcum transgressed wheat line as QYrtm.pau-2A [66], and in wheat genotype "Aquileja" [21]; and by three genes or QTL was reported in "Guardian" [67]. In winter wheat "IDO444", HTAP resistance is controlled by two major QTL and three minor QTL [56].…”