QTL Mapping and Validation of Adult Plant Resistance to Stripe Rust in Chinese Wheat Landrace Humai 15

Yuan, Fengping; Zeng, Qingdong; Wu, Jianhui; Wang, Qilin; Yang, Zujun; Liang, Bang-Ping; Chen, Xinhong

doi:10.3389/fpls.2018.00968

Cited by 59 publications

(44 citation statements)

References 53 publications

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“…In addition, structural changes in wheat chromosomes can be detected by FISH. For example, FISH was used to confirm a reciprocal translocation of the 5BL-7BS chromosome in a wheat variety Humai15 and a reciprocal translocation of the 4BL-6AL chromosome in a wheat variety Mingxian 169 [44]. In this study, however, FISH did not detect 5DL-5NsL.5NsS chromosome, therefore, 5NsL·5NsS might undergo another translocation within wheat chromosome, which is confirmed by FISH identification of two translocations, 3DS-5NsL·5NsS and 5DL-3DS·3DL in TR77.…”

Section: Discussionsupporting

confidence: 49%

Molecular cytogenetic characterization of a novel wheat–Psathyrostachys huashanica Keng T3DS-5NsL•5NsS and T5DL-3DS•3DL dual translocation line with powdery mildew resistance

Zhao

Cheng

et al. 2020

BMC Plant Biol

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Background: Psathyrostachys huashanica Keng (2n = 2x = 14, NsNs) carries many outstanding agronomic traits, therefore is a valuable resource for wheat genetic improvement. Wheat-P. huashanica translocation lines are important intermediate materials for wheat breeding and studying the functions of alien chromosomes. However, powdery mildew resistance in these translocation lines has not been reported previously.Results: This study developed a novel wheat-P. huashanica translocation line TR77 by selecting a F 7 progeny from the cross between heptaploid hybrid H8911 (2n = 7x = 49, AABBDDNs) and durum wheat line Trs-372. Chromosome karyotype of 2n = 42 = 21II was observed in both mitotic and meiotic stages of TR77. Genomic in situ hybridization analysis identified two translocated chromosomes that paired normally at meiosis stage in TR77. Molecular marker analysis showed that part of chromosome 5D was replaced by part of alien chromosome fragment 5Ns. It meant replacement made part 5DL and part 5NsL·5NsS existed in wheat background, and then translocation happened between these chromosomes and wheat 3D chromosome. Fluorescence in situ hybridization demonstrated that TR77 carries dual translocations: T3DS-5NsL·5NsS and T5DL-3DS·3DL. Analysis using a 15 K-wheat-SNP chip confirmed that SNP genotypes on the 5D chromosome of TR77 matched well with these of P. huashanica, but poorly with common wheat line 7182. The translocation was physically located between 202.3 and 213.1 Mb in 5D. TR77 showed longer spikes, more kernels per spike, and much better powdery mildew resistance than its wheat parents: common wheat line 7182 and durum wheat line Trs-372.(Continued on next page)

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Section: Discussionsupporting

confidence: 49%

Molecular cytogenetic characterization of a novel wheat–Psathyrostachys huashanica Keng T3DS-5NsL•5NsS and T5DL-3DS•3DL dual translocation line with powdery mildew resistance

Zhao

Cheng

et al. 2020

BMC Plant Biol

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“…IT numbers of 0, 1-3, 4-6, and 7-9 were considered immune, resistant, intermediate, and susceptible, respectively. DS was recorded as the percentage of the diseased leaf area [43,50]. For the F 5 plants, we selected resistant plants with full spikes, plant height less than 1 m, and a tiller number of 4 or greater.…”

Section: Disease Resistance and Agronomic Trait Evaluation In The Fieldmentioning

confidence: 99%

Introgression of Two Quantitative Trait Loci for Stripe Rust Resistance into Three Chinese Wheat Cultivars

Zhong

Zhou

et al. 2020

Agronomy

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Wheat stripe rust, caused by Puccinia striiformis f. sp. tritici (Pst), is one of the most devastating diseases in wheat. Due to the large-scale and widely-distributed planting pattern of wheat, the directional selection pressure of the pathogen is very strong. Therefore, it is urgent to pyramid more stripe rust resistance genes in wheat cultivars to enhance resistance durability and ensure wheat production safety. In this study, two quantitative trait loci (QTL) for adult plant resistance (APR) to stripe rust, QYr.nafu-2BL and QYr.nafu-3BS, were validated and introgressed from wheat line P9897 into three Chinese elite wheat cultivars, Chuanmai 42, Xiangmai 25, and Zhengmai 9023, through marker validation. The three Chinese elite varieties were used as the female parent to cross with wheat line P9897, and they were selfed to the F6 generation. A total of 114 lines were then selected based on field agronomic traits and stripe rust resistance. Four markers (Xcfd73, Xgwm120, Xbarc87 and Xbarc133) linked with the QTL’s regions were employed to screen the 114 F6 lines. Subsequently, 27 lines combining two target QTL from P9897 were selected. The combination of agronomic traits and disease resistance results showed that 13 of these selected lines had favorable application prospects. The promising lines selected in this study could enrich the genetic resources of wheat stripe rust resistance genes, as well as provide material support and a theoretical basis for the prevention and control of wheat stripe rust in China.

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“…Association analysis of stripe rust resistance using the general linear model + population structure (GLM + PC) SNP ID Chro. P-value Target allele (1) Allele effect (2) The left allele increased the resistance, (2) The effect of the left allele associated with increasing the resistance, (3) Phenotypic variation explained by marker. Number of markers signi cantly associated with the resistance to the Egyptian race of stripe rust at adult growth stage in 2019 and 2020 and the common markers between both years: a. signi cant SNP markers based on the GWAS using GLM+PC model, b. signi cant DArT markers based on single marker analysis (SMA).…”

Section: Genome-wide Association Study (Gwas) Single Marker Analysismentioning

confidence: 99%

“…Tritici), also known as yellow rust, is one of the most devastating wheat diseases in the world. It can cause losses in yield from 10 to 100% [1]. New stripe rust races appeared around the year 2000, spread rapidly, and caused signi cant yield losses [2,3].…”

Section: Introductionmentioning

confidence: 99%

“…In the recent study, we used 103-spring wheat genotypes which were collected from 14 different countries all over the world and are well adapted to the Egyptian environment (Dr. Ahmed Sallam, personal communication). The objectives of this study were to (1) understand the stripe rust resistance in Egyptian and worldwide wheat genotypes, (2) identify allele markers associated with the resistance to stripe rust Egyptian race, and (3) select the best genotypes to be used in future breeding programs to improve wheat resistant to stripe rust.…”

Section: Introductionmentioning

confidence: 99%

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Genomic Regions Associated With Stripe Rust Resistance Against the Egyptian Race Revealed by Genome-Wide Association Study

Mohamed

Mourad

2020

Preprint

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Background: Wheat stripe rust (caused by Puccinia striiformis f. sp. Tritici), is a major disease that causes huge yield damage. New pathogen races appeared in the last few years and caused a broke down in the resistant genotypes. In Egypt, some of the resistant genotypes began to be susceptible to stripe rust in recent years. This situation increases the need to produce new genotypes with durable resistance. Besides, looking for a new resistant source from the available wheat genotypes all over the world help in enhancing the breeding programs. Results: In the recent study, a set of 103-spring wheat genotypes from different fourteen countries were evaluated to their field resistant to stripe rust for two years. These genotypes included 17 Egyptian genotypes from the old and new cultivars. The 103-spring wheat genotypes were reported to be well adapted to the Egyptian environmental conditions. Out of the tested genotypes, eight genotypes from four different countries were found to be resistant in both years. Genotyping was carried out using genotyping-by-sequencing and a set of 26,703 SNPs were used in the genome-wide association study. Five SNP markers, located on chromosomes 2A and 4A, were found to be significantly associated with the resistance in both years. Three gene models associated with disease resistance and underlying these significant SNPs were identified. One immune Iranian genotype, with the highest number of different alleles from the most resistant Egyptian genotypes, was detected. Conclusion: the high variation among the tested genotypes in their resistance to the Egyptian stripe rust race confirming the possible improvement of stripe rust resistance in the Egyptian wheat genotypes. The identified five SNP markers are stable and could be used in marker-assisted selection after validation in different genetic backgrounds. Crossing between the immune Iranian genotype and the Egyptian genotypes will improve stripe rust resistance in Egypt.

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QTL Mapping and Validation of Adult Plant Resistance to Stripe Rust in Chinese Wheat Landrace Humai 15

Cited by 59 publications

References 53 publications

Molecular cytogenetic characterization of a novel wheat–Psathyrostachys huashanica Keng T3DS-5NsL•5NsS and T5DL-3DS•3DL dual translocation line with powdery mildew resistance

Molecular cytogenetic characterization of a novel wheat–Psathyrostachys huashanica Keng T3DS-5NsL•5NsS and T5DL-3DS•3DL dual translocation line with powdery mildew resistance

Introgression of Two Quantitative Trait Loci for Stripe Rust Resistance into Three Chinese Wheat Cultivars

Genomic Regions Associated With Stripe Rust Resistance Against the Egyptian Race Revealed by Genome-Wide Association Study

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