QTL mapping for haploid male fertility by a segregation distortion method and fine mapping of a key QTL qhmf4 in maize

Ren, Jiaojiao; Wu, Penghao; Tian, Xiaolong; Lübberstedt, Thomas; Chen, Shaojiang

doi:10.1007/s00122-017-2892-6

Cited by 32 publications

(84 citation statements)

References 49 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The detected HMF QTL in our study did not completely match those QTL reported previously (Table S3). Based on physical coordinates from reference sequences, there is an overlap of HMF QTL with flanking markers umc2266‐umc2268 (present study), bnlg1035‐umc1528 (Ren et al, ) and umc1539‐umc1528 (Wu, ) on chromosome 3, as well as umc1409‐dupssr9 (present study) and SNP Chr7.S_113165535 (Ma et al, ) on chromosome 7, umc1607‐phi080 (present study) and umc1997‐dupssr14 (Wu, ) on chromosome 8. To date, 34 QTL related to HMF have been detected across 10 chromosomes in the present and previous studies.…”

Section: Discussionmentioning

confidence: 53%

“…There is no uniform standard to measure the characteristics of haploid fertility restoration. Kleiber et al () and Ren et al () scored anther emergence and classified haploids using a 5‐point scale based on (score 1) less than 5%, 6%–20% (score 2), 21%–50% (score 3), 51%–75% (score 4) and 76%–100% (score 5) of anthers emerged on the tassel. Chalyk () and Geiger et al () assessed shedding efficiency in their studies.…”

Section: Discussionmentioning

confidence: 99%

“…Only the locus on chromosome 8 was detected in both years. Ren et al () reported four HMF QTL, qhmf1 , qhmf2 , qhmf3 and qhmf4 , identified by segregation distortion. QTL detection was done in the selected haploid population derived from "Yu87–1/Zheng58", and 48 recombinants were used to narrow the qhmf4 locus down to a ~800 kb interval flanked by markers IND166 and IND1668.…”

Section: Discussionmentioning

confidence: 99%

“…Wu () reported no significant differences in HMF restoration rates between reciprocal crosses. Ren, Wu, Tian, Lübberstedt, and Chen () reported four quantitative trait loci (QTL) related to HMF and found a major QTL on chromosome 6. Recently, Ma et al () detected 14 single‐nucleotide polymorphisms (SNPs) significantly associated with HMF according to the genome‐wide association study (GWAS).…”

Section: Introductionmentioning

confidence: 99%

“…So far, only few studies addressed the genetic basis of HMF (Chalyk, ; Ren et al, ; Testillano et al, ). Ren et al () first reported the QTL related to HMF using two segregation populations from temperate germplasm Zheng58 crossed with tropical Yu87‐1 and Lancaster germplasm 4F 1 . In this study, we used F 2:3 families from the cross of inbred lines Zheng58 and K22, which both from temperate germplasm to determine HMF rates in two different locations across two seasons.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Genetic dissection of haploid male fertility in maize (Zea mays L.)

Yang

Chen

et al. 2019

Plant Breeding

Self Cite

View full text Add to dashboard Cite

Haploid genome doubling is a key limiting step of haploid breeding in maize. Spontaneous restoration of haploid male fertility (HMF) provides a more promising method than the artificial doubling process. To reveal the genetic basis of HMF, haploids were obtained from the offspring of 285 F2:3 families, derived from the cross Zheng58 × K22. The F2:3 families were used as the female donor and Yu high inducer No. 1 (YHI‐1) as the male inducer line. The rates of HMF from each family line were evaluated at two field sites over two planting seasons. HMF displayed incomplete dominance. Transgressive segregation of haploids from F2:3 families was observed relative to haploids derived from the two parents of the mapping population. A total of nine quantitative trait loci (QTL) were detected, which were distributed on chromosomes 1, 3, 4, 7 and 8. Three major QTL, qHMF3b, qHMF7a and qHMF7b were detected in both locations, respectively. These QTL could be useful to predict the ability of spontaneous haploid genome doubling, and to accelerate the haploid breeding process by introgression or aggregation of those QTL.

show abstract

Section: Discussionmentioning

confidence: 53%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Genetic dissection of haploid male fertility in maize (Zea mays L.)

Yang

Chen

et al. 2019

Plant Breeding

Self Cite

View full text Add to dashboard Cite

show abstract

A diallel analysis of a maize donor population response to in vivo maternal haploid induction: II. Haploid male fertility

et al. 2020

Self Cite

View full text Add to dashboard Cite

Doubled haploid (DH) lines are used in maize (Zea mays L.) breeding to accelerate the breeding cycle and create homogenous inbred lines in as little as two seasons. These pure inbred lines allow breeders to quickly evaluate new cross combinations. There are two important steps in creating DH lines: (a) generation and selection of haploid progeny, and (b) genome doubling to create fertile, diploid inbreds. Colchicine is widely used to artificially double genomes in haploid plants, which is hazardous, expensive, and time consuming. In this study, three public inbred lines-A427, A637, and NK778-were found to have substantial haploid male fertility (HMF). A sixparent full diallel between these three HMF lines and three non-HMF lines was created and HMF was scored. Diallel analysis revealed significant general combining ability (GCA) estimates of up to 17% for HMF, as well as significant specific combining ability (SCA) effects of up to 25%. No significant reciprocal effects were found. Haploid male fertility is promising to be incorporated into elite maize breeding programs to potentially overcome the need of using colchicine treatments for genome doubling. Colchicine aided doubling success rates varying from almost 0 to 30%. Haploid male fertility has an advantage over artificial genome doubling, in terms of both increased success rates and decreased costs for DH line production.

show abstract

Doubled haploids in maize: Development, deployment, and challenges

Maqbool¹,

Beshir²,

Khokhar³

2020

Crop Science

View full text Add to dashboard Cite

Haploids are naturally produced in maize (Zea mays L.) at different rates and can also be induced through different methods. Haploids are used to develop doubled haploids (DHs), which have many potential uses. The development of DH lines in maize involves haploid induction, haploid identification, chromosome doubling, and field sowing for self‐pollination of D0 plants. Different potential methods are used for haploid induction, in‐vivo maternal haploid induction being the most prevalent. Haploid induction is highly reliant on the unambiguous identification of haploids among a mixture of different ploidies. Haploid identification is facilitated by visual morphological markers, chromosome counting, flow cytometry, molecular markers, and many other approaches. Chromosome doubling may be achieved by spontaneous doubling or by induction with different antimicrotubular treatments. Among the potential uses of DH lines are the development of inbred lines, genomic selection (GS), quantitative trait loci (QTL) mapping, and unlocking new genetic variations. Although DH technology can potentially accelerate maize breeding, it still faces challenges at each step of DH line development. This article aims to highlight the importance, procedural steps, potential opportunities, and key challenges in DH line development in maize.

show abstract

QTL mapping for haploid male fertility by a segregation distortion method and fine mapping of a key QTL qhmf4 in maize

Cited by 32 publications

References 49 publications

Genetic dissection of haploid male fertility in maize (Zea mays L.)

Genetic dissection of haploid male fertility in maize (Zea mays L.)

A diallel analysis of a maize donor population response to in vivo maternal haploid induction: II. Haploid male fertility

Doubled haploids in maize: Development, deployment, and challenges

Contact Info

Product

Resources

About