QTL mapping for root vigor and days to flowering in Brassica napus L.

Variation in root architecture is essential for the adaptation of plants to target environments. A non-destructive gel-based mini-rhizotron system was used for root architecture trait phenotyping. This system has facilitated the visualization of root architectural traits in large genotype collection of rapeseed including 94 double haploid (DH) lines from "Express617" x "V8" and 439 inbred lines (ASSYST diversity set). A high-density Express617-V8 linkage map was used for quantitative trait loci (QTLs) identification in DH population based on standard composite interval mapping. 6K SNPs were analysed for association mapping of root traits in ASSYST diversity set. A large variation, broad segregation and medium-low heritability of root architectural traits, primary root length and growth rate, lateral root number, lateral root length and lateral root density, were observed. In the double haploid population, 11 QTL regions, and in the diversity set, 38 significant marker-trait associations were detected. Significant marker-trait associations proved that these are quantitatively inherited traits controlled by multiple genes which revealed to proceed for genetic improvement and selection of rapeseed lines with improved root system. K E Y W O R D S

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“…ArifUzZaman et al. () identify one QTL for root vigour explaining 16% of the phenotypic variation on chromosome A1 (24.7 Mbp).…”

Section: Discussionmentioning

confidence: 99%

“…() reported a total of 131 QTLs for root architectural traits in rapeseed under contrasting P application. A QTL mapping for napus root vigour was identified on chromosome A01 (24.7 Mbp) explaining 16.3% of the phenotypic variation (Arifuzzaman, Mamidi, McClean, & Rahman, ). Furthermore, Wang et al.…”

Section: Introductionmentioning

confidence: 99%

Genetic dissection of root architectural traits by QTL and genome‐wide association mapping in rapeseed (Brassica napus)

et al. 2019

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“…The allelic frequencies for the two DNA bulks are then estimated. The application of genetic studies to rapeseed root traits relies on a phenotyping procedure, which requires growing plants on agar-based medium (Shi et al, 2012;Kiran et al, 2019;Kupcsik et al, 2021), in hydroponics (Zhang et al, 2016;Wang et al, 2017a) or in soil (Arifuzzaman et al, 2016(Arifuzzaman et al, , 2019Louvieaux et al, 2020a).…”

Section: Introductionmentioning

confidence: 99%

Genetic control of root morphology in response to nitrogen across rapeseed diversity

Haelterman,

Louvieaux,

Chiodi

et al. 2024

Physiologia Plantarum

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Rapeseed (Brassica napus L.) is an oil‐containing crop of great economic value but with considerable nitrogen requirement. Breeding root systems that efficiently absorb nitrogen from the soil could be a driver to ensure genetic gains for more sustainable rapeseed production. The aim of this study is to identify genomic regions that regulate root morphology in response to nitrate availability. The natural variability offered by 300 inbred lines was screened at two experimental locations. Seedlings grew hydroponically with low or elevated nitrate levels. Fifteen traits related to biomass production and root morphology were measured. On average across the panel, a low nitrate level increased the root‐to‐shoot biomass ratio and the lateral root length. A large phenotypic variation was observed, along with important heritability values and genotypic effects, but low genotype‐by‐nitrogen interactions. Genome‐wide association study and bulk segregant analysis were used to identify loci regulating phenotypic traits. The first approach nominated 319 SNPs that were combined into 80 QTLs. Three QTLs identified on the A07 and C07 chromosomes were stable across nitrate levels and/or experimental locations. The second approach involved genotyping two groups of individuals from an experimental F2 population created by crossing two accessions with contrasting lateral root lengths. These individuals were found in the tails of the phenotypic distribution. Co‐localized QTLs found in both mapping approaches covered a chromosomal region on the A06 chromosome. The QTL regions contained some genes putatively involved in root organogenesis and represent selection targets for redesigning the root morphology of rapeseed.

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“…Rapeseed absorbs between 290 to 373 kg ha −1 of K, with the majority of the K coming from the soil. Some studies in B. napus have focused on root traits, specifically the root development at the seedling stage; root vigor; root development at the mature stage; root growth dynamics; variations in root growth habits of spring, winter, and semi-winter rapeseed; and the significance of root morphology in terms of phosphorus and nitrogen absorption [35][36][37][38][39][40][41][42][43]. So far, few studies have identified genetic pathways or genes associated with the architecture of the root system in B. napus grown in low-potassium environments.…”

Section: Introductionmentioning

confidence: 99%

Genome-Wide Association Studies of Root-Related Traits in Brassica napus L. under Low-Potassium Conditions

Ibrahim

Ahmad

Kuang

et al. 2022

Plants

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Roots are essential organs for a plant’s ability to absorb water and obtain mineral nutrients, hence they are critical to its development. Plants use root architectural alterations to improve their chances of absorbing nutrients when their supply is low. Nine root traits of a Brassica napus association panel were explored in hydroponic-system studies under low potassium (K) stress to unravel the genetic basis of root growth in rapeseed. The quantitative trait loci (QTL) and candidate genes for root development were discovered using a multilocus genome-wide association study (ML-GWAS). For the nine traits, a total of 453 significant associated single-nucleotide polymorphism (SNP) loci were discovered, which were then integrated into 206 QTL clusters. There were 45 pleiotropic clusters, and qRTA04-4 and qRTC04-7 were linked to TRL, TSA, and TRV at the same time, contributing 5.25–11.48% of the phenotypic variance explained (PVE) to the root traits. Additionally, 1360 annotated genes were discovered by examining genomic regions within 100 kb upstream and downstream of lead SNPs within the 45 loci. Thirty-five genes were identified as possibly regulating root-system development. As per protein–protein interaction analyses, homologs of three genes (BnaC08g29120D, BnaA07g10150D, and BnaC04g45700D) have been shown to influence root growth in earlier investigations. The QTL clusters and candidate genes identified in this work will help us better understand the genetics of root growth traits and could be employed in marker-assisted breeding for rapeseed adaptable to various conditions with low K levels.

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QTL mapping for root vigor and days to flowering in Brassica napus L.

Cited by 17 publications

References 76 publications

Genetic dissection of root architectural traits by QTL and genome‐wide association mapping in rapeseed (Brassica napus)

Genetic dissection of root architectural traits by QTL and genome‐wide association mapping in rapeseed (Brassica napus)

Genetic control of root morphology in response to nitrogen across rapeseed diversity

Genome-Wide Association Studies of Root-Related Traits in Brassica napus L. under Low-Potassium Conditions

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