QTL mapping of adult plant resistance to Ug99 stem rust in the spring wheat population RB07/MN06113-8

Bajgain, Prabin; Rouse, Matthew N.; Bhavani, Sridhar; Anderson, James A.

doi:10.1007/s11032-015-0362-x

Cited by 18 publications

(23 citation statements)

References 67 publications

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“…umn-7A , the only QTL on 7A, is located at the very beginning (3 cM) of the linkage group 7A as well as on the physical map (ranging 2–6 Mb). This is strikingly similar to previous QTL reported on 7AS by Bajgain et al [ 75 ] and Singh et al [ 65 ], and could represent the same locus. Chromosome 7B also had only one QTL detected ( QSr .…”

Section: Resultssupporting

confidence: 92%

Nested Association Mapping of Stem Rust Resistance in Wheat Using Genotyping by Sequencing

et al. 2016

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We combined the recently developed genotyping by sequencing (GBS) method with joint mapping (also known as nested association mapping) to dissect and understand the genetic architecture controlling stem rust resistance in wheat (Triticum aestivum). Ten stem rust resistant wheat varieties were crossed to the susceptible line LMPG-6 to generate F6 recombinant inbred lines. The recombinant inbred line populations were phenotyped in Kenya, South Africa, and St. Paul, Minnesota, USA. By joint mapping of the 10 populations, we identified 59 minor and medium-effect QTL (explained phenotypic variance range of 1% – 20%) on 20 chromosomes that contributed towards adult plant resistance to North American Pgt races as well as the highly virulent Ug99 race group. Fifteen of the 59 QTL were detected in multiple environments. No epistatic relationship was detected among the QTL. While these numerous small- to medium-effect QTL are shared among the families, the founder parents were found to have different allelic effects for the QTL. Fourteen QTL identified by joint mapping were also detected in single-population mapping. As these QTL were mapped using SNP markers with known locations on the physical chromosomes, the genomic regions identified with QTL could be explored more in depth to discover candidate genes for stem rust resistance. The use of GBS-derived de novo SNPs in mapping resistance to stem rust shown in this study could be used as a model to conduct similar marker-trait association studies in other plant species.

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Section: Resultssupporting

confidence: 92%

Nested Association Mapping of Stem Rust Resistance in Wheat Using Genotyping by Sequencing

et al. 2016

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“…The gene Sr11 is located on 6BL, but is ineffective to Ug99 and its derivative races [ 3 , 5 ]. Likewise, several QTL have been reported on 7A that provide field resistance to Ug99 [ 46 , 47 , 51 , 52 , 68 ]. However, none of the reported QTL or positions of significant marker effects coincide with the significant markers detected in this study.…”

Section: Discussionmentioning

confidence: 99%

“…Additionally, the gene Sr1RS Amigo is located on the 1RS.1AL rye chromosome arm translocation. Chromosome 7AS does not possess any known ASR genes, yet APR QTL effective to Ug99 and its derivative races have been detected in the region [ 49 , 68 ].…”

Section: Discussionmentioning

confidence: 99%

Association mapping of North American spring wheat breeding germplasm reveals loci conferring resistance to Ug99 and other African stem rust races

et al. 2015

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BackgroundThe recently identified Puccinia graminis f. sp. tritici (Pgt) race TTKSK (Ug99) poses a severe threat to global wheat production because of its broad virulence on several widely deployed resistance genes. Additional virulences have been detected in the Ug99 group of races, and the spread of this race group has been documented across wheat growing regions in Africa, the Middle East (Yemen), and West Asia (Iran). Other broadly virulent Pgt races, such as TRTTF and TKTTF, present further difficulties in maintaining abundant genetic resistance for their effective use in wheat breeding against this destructive fungal disease of wheat. In an effort to identify loci conferring resistance to these races, a genome-wide association study was carried out on a panel of 250 spring wheat breeding lines from the International Maize and Wheat Improvement Center (CIMMYT), six wheat breeding programs in the United States and three wheat breeding programs in Canada.ResultsThe lines included in this study were grouped into two major clusters, based on the results of principal component analysis using 23,976 SNP markers. Upon screening for adult plant resistance (APR) to Ug99 during 2013 and 2014 in artificial stem rust screening nurseries at Njoro, Kenya and at Debre Zeit, Ethiopia, several wheat lines were found to exhibit APR. The lines were also screened for resistance at the seedling stage against races TTKSK, TRTTF, and TKTTF at USDA-ARS Cereal Disease Laboratory in St. Paul, Minnesota; and only 9 of the 250 lines displayed seedling resistance to all the races. Using a mixed linear model, 27 SNP markers associated with APR against Ug99 were detected, including markers linked with the known APR gene Sr2. Using the same model, 23, 86, and 111 SNP markers associated with seedling resistance against races TTKSK, TRTTF, and TKTTF were identified, respectively. These included markers linked to the genes Sr8a and Sr11 providing seedling resistance to races TRTTF and TKTTF, respectively. We also identified putatively novel Sr resistance genes on chromosomes 3B, 4D, 5A, 5B, 6A, 7A, and 7B.ConclusionOur results demonstrate that the North American wheat breeding lines have several resistance loci that provide APR and seedling resistance to highly virulent Pgt races. Using the resistant lines and the SNP markers identified in this study, marker-assisted resistance breeding can assist in development of varieties with elevated levels of resistance to virulent stem rust races including TTKSK.Electronic supplementary materialThe online version of this article (doi:10.1186/s12870-015-0628-9) contains supplementary material, which is available to authorized users.

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“…As an example, QTL region 16 on chromosome 6A, was previously identified in biparental populations by [34], and more recently in association mapping panel of durum wheat cultivars by [1,26]. For QTL region 10 on chromosome 4A (Table S1), loci for stem rust resistance were previously identified in both durum and bread wheat, and against several stem rust races, such as TTKSK [26,35,36], TKTTF [37], and TTTTF [38]. Two Sr genes were also mapped to this region: Sr7 and SrND643 [36] (Figure 3).…”

Section: Discussionmentioning

confidence: 99%

Genetic Mapping of Loci for Resistance to Stem Rust in a Tetraploid Wheat Collection

Saccomanno

Matny

Marone

et al. 2018

IJMS

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Stem rust, caused by Puccinia graminis f. sp. tritici (Pgt), is a major biotic constraint to wheat production worldwide. Disease resistant cultivars are a sustainable means for the efficient control of this disease. To identify quantitative trait loci (QTLs) conferring resistance to stem rust at the seedling stage, an association mapping panel consisting of 230 tetraploid wheat accessions were evaluated for reaction to five Pgt races under greenhouse conditions. A high level of phenotypic variation was observed in the panel in response to all of the races, allowing for genome-wide association mapping of resistance QTLs in wild, landrace, and cultivated tetraploid wheats. Twenty-two resistance QTLs were identified, which were characterized by at least two marker-trait associations. Most of the identified resistance loci were coincident with previously identified rust resistance genes/QTLs; however, six regions detected on chromosomes 1B, 5A, 5B, 6B, and 7B may be novel. Availability of the reference genome sequence of wild emmer wheat accession Zavitan facilitated the search for candidate resistance genes in the regions where QTLs were identified, and many of them were annotated as NOD (nucleotide binding oligomerization domain)-like receptor (NLR) genes or genes related to broad spectrum resistance.

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QTL mapping of adult plant resistance to Ug99 stem rust in the spring wheat population RB07/MN06113-8

Cited by 18 publications

References 67 publications

Nested Association Mapping of Stem Rust Resistance in Wheat Using Genotyping by Sequencing

Nested Association Mapping of Stem Rust Resistance in Wheat Using Genotyping by Sequencing

Association mapping of North American spring wheat breeding germplasm reveals loci conferring resistance to Ug99 and other African stem rust races

Genetic Mapping of Loci for Resistance to Stem Rust in a Tetraploid Wheat Collection

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