QTL mapping of fruit nutritional and flavor components in tomato (Solanum lycopersicum) using genome-wide SSR markers and recombinant inbred lines (RILs) from an intra-specific cross

Kimbara, Junji; Ohyama, Akio; Chikano, Hiroshi; Ito, Hirotaka; Hosoi, Katsutoshi; Negoro, Satomi; Miyatake, Koji; Yamaguchi, Haruhisa; Nunome, Toshiro; Fukuoka, Hiroyuki; Hayashi, Takeshi

doi:10.1007/s10681-018-2295-z

Cited by 14 publications

(10 citation statements)

References 62 publications

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“…Our results indicate that intake can be increased by choosing a high lycopene cultivar. At present, new molecular tools are available for marker-assisted selection [32]. Surprisingly, no correlation was observed between fruit weight and yield (Table 3).…”

Section: Discussionmentioning

confidence: 99%

Heritability and Variability of Quality Parameters of Tomatoes in Outdoor Production

et al. 2020

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The tomato is the most important vegetable globally. In England, outdoor tomatoes are widely grown by amateur gardeners, with the number of their allotment plots exceeding 150000. For instance, in Germany, only about 16% of tomato plants are cultivated according to organic standards, although these rates are expected to increase. Breeding for yield and fruit quality can increase resource efficiency. Therefore, we need to evaluate the variability of yield and fruit quality parameters, to calculate the heritability of these traits and to identify superior genotypes for organic outdoor tomato production and breeding. With these aims, we grew 24 tomato genotypes of diverse origins in a two-year field trial. The heritability of quality traits such as glucose, fructose, organic acid, and lycopene was high. The medium heritability for yield suggests that trials with a larger number of locations are needed for the reliable selection of this character. Negative correlations of sugar concentrations with fruit weight and of organic acid concentration with fruit weight and yield suggest trade-offs in breeding for larger fruits and higher yields. Breeding for increased lycopene content is not subject to these challenges; the concentrations of the primary metabolite sugars and organic acids are positively correlated.

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Section: Discussionmentioning

confidence: 99%

Heritability and Variability of Quality Parameters of Tomatoes in Outdoor Production

et al. 2020

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“…Field-grown tomatoes have been reported to have higher levels of VOCs than greenhouse-grown fruits ( 12 ), but some cultivars e.g., Campari F 1 , have been developed for superior flavor in protected cropping. Flavor properties are important breeding targets to meet consumer demands for tomatoes ( 13 , 14 ). In addition, the increasing interest in environmentally friendly produced food ( 15 ) may encourage breeders to develop cultivars with excellent flavor characteristics for organic low-input cultivation as well.…”

Section: Introductionmentioning

confidence: 99%

Flavor and Other Quality Traits of Tomato Cultivars Bred for Diverse Production Systems as Revealed in Organic Low-Input Management

et al. 2022

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This study was conducted to determine the volatile organic compounds (VOCs) associated with fruit flavor in diverse tomato cultivars (salad and cocktail cultivars) under organic low-input production. For this objective, 60 cultivars deriving from very diverse breeding programs 1880–2015 were evaluated in 2015, and a subset of 20 cultivars was selected for further evaluation in 2016. The diversity of instrumentally determined traits, especially for VOCs concentration and sensory properties (fruit firmness, juiciness, skin firmness, sweetness, sourness, aroma, and acceptability), was investigated at two harvest dates. The evaluation of the cultivars exhibited a wide range of variation for all studied traits, with the exception of a few VOCs. Cultivar had the most important effect on all instrumentally determined traits, while the influence of cultivar × harvest date × year interaction was significant for 17 VOCs, but not for total soluble solid (TSS) and titratable acidity (TA). The VOCs with the highest proportion (>8%) were hexanal, 6-methyl-5-heptene-2-one, 2-isobutylthiazole, and (E)-2-hexenal, which were identified in all cultivars. Twelve VOCs significantly correlated with one or more sensory attributes and these VOCs also allowed differentiation of the fruit type. Among these VOCs, phenylethyl alcohol and benzyl alcohol positively correlated with acceptability in the cocktail cultivars, whereas 2-isobuthylthiazole and 6-methyl-5-hepten-2-ol negatively correlated with acceptability in the salad cultivars. As a result of this study, organic breeders are recommended to use cultivars from a wide range of breeding programs to improve important quality and agronomic traits. As examples, salad tomatoes “Campari F1”, “Green Zebra”, and “Auriga”, as well as cocktail tomatoes “Supersweet 100 F1”, “Sakura F1”, and “Black Cherry” showed higher scores for the sensory attributes aroma and acceptability under organic low-input growing conditions. It remains a challenge for breeders and growers to reduce the trade-off of yield and quality.

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“…Several tomato genes with strong ripening phenotypes have been identified via mutagenesis-based breeding programs including rin , nor , NR , and Cnr ( Tigchelaar, 1973 ; Lanahan et al, 1994 ; Vrebalov et al, 2002 ; Manning et al, 2006 ). Moreover, the recombinant inbred line (RILs) population is also useful to identify key loci regulating quantitative traits including fruit ripening as well as aroma, color, and disease resistance ( Kimbara et al, 2018 ). However, since RILs usually harbor more than one introgression, the possibility of the introgressed loci having beneficial/inhibitory interactions with the genes in the genetic background renders gene function elucidation more complex in RILs.…”

Section: Discussionmentioning

confidence: 99%

A comparative transcriptomics and eQTL approach identifiesSlWD40as a tomato fruit ripening regulator

et al. 2022

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Although multiple vital genes with strong effects on the tomato (Solanum lycopersicum) ripening process have been identified via the positional cloning of ripening mutants and cloning of ripening-related transcription factors, recent studies suggest that it is unlikely that we have fully characterized the gene regulatory networks underpinning this process. Here, combining comparative transcriptomics and eQTLs, we identified 16 candidate genes involved in tomato fruit ripening and validated them through virus-induced gene silencing (VIGS) analysis. To further confirm the accuracy of the approach, one potential ripening regulator, SlWD40 (WD-40 repeats), was chosen for in-depth analysis. Co-expression network analysis indicated that master regulators such as RIN (ripening inhibitor) and NOR (nonripening) as well as vital transcription factors including FUL1 (FRUITFUL1), SlNAC4 (NAM, ATAF1,2 and CUC2 4), and AP2a (Activating enhancer binding Protein 2 alpha) strongly co-expressed with SlWD40. Furthermore, SlWD40 overexpression and RNAi lines exhibited substantially accelerated and delayed ripening phenotypes compared to the WT, respectively. Moreover, transcriptome analysis of these transgenics revealed that expression patterns of ethylene biosynthesis genes, phytoene synthase (PSY), pectate lyase (PL), and branched chain amino transferase 2 (BCAT2), in SlWD40-RNAi lines were similar to those of rin and nor fruits, which further demonstrated that SlWD40 may act as an important ripening regulator in conjunction with RIN and NOR. These results are discussed in the context of current models of ripening and in terms of the use of comparative genomics and transcriptomics as an effective route for isolating causal genes underlying differences in genotypes.

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QTL mapping of fruit nutritional and flavor components in tomato (Solanum lycopersicum) using genome-wide SSR markers and recombinant inbred lines (RILs) from an intra-specific cross

Cited by 14 publications

References 62 publications

Heritability and Variability of Quality Parameters of Tomatoes in Outdoor Production

Heritability and Variability of Quality Parameters of Tomatoes in Outdoor Production

Flavor and Other Quality Traits of Tomato Cultivars Bred for Diverse Production Systems as Revealed in Organic Low-Input Management

A comparative transcriptomics and eQTL approach identifiesSlWD40as a tomato fruit ripening regulator

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