QTL Mapping of Seed Vigor of Backcross Inbred Lines Derived From Oryza longistaminata Under Artificial Aging

Abstract

Seed vigor is an important character of seed quality that promotes rice to germinate rapidly from soil and developing to a strong seedling, especially in the current rice direct-sowing production system. However, previous studies for seed vigor mainly concentrate in cultivars, and less reports involving in wild rice. In this study, 152 backcross inbred lines (BILs) derived from wild rice Oryza longistaminata were genotyped with re-sequencing technology, and QTLs for seed vigor related traits under normal and artificial aging treatment were analyzed. Totally, 36 QTLs were detected, of which, eight for germination potential (GP), 10 for germination rate (GR), 9 for seedling length (SL), and 9 for root length (RL). Among these, 14 novel QTLs were identified from O. longistaminata. Of which, six QTLs were related to germination, and eight related to seedling growth under aging stress. What’s more, the major QTLs q9SL1.1, q6SL1.1, and q3SL1.1 for seedling length were fallen in the same locus and fine-mapped an interval about 90 Kb. The major QTLs q9GR8.1 and q9GP8.1 related with germination were fine-mapped to an interval about 90 Kb. This work will provide us basis for breeding of high seed vigor rice in rice breeding programs and further cloning of these genes.

Quantitative Trait Locus Analysis of Seed Germination and Early Seedling Growth in Rice

“…Seed germination is a complex process that can be influenced by many factors, such as seed size, genotype, seed development, storage time and environment (Sun et al, 2007;Milošević et al, 2010). To date, numerous quantitative trait loci (QTLs) for seed germination have been reported in rice (Miura et al, 2002;Fujino et al, 2004;Jiang et al, 2006;Fujino et al, 2008;Wang et al, 2010;Li et al, 2013;Liu et al, 2014;Xie et al, 2014;Cheng et al, 2015;Hsu and Tung, 2015;Kretzschmar et al, 2015;Jiang et al, 2017;Wang et al, 2017;Jin et al, 2018). However, cloned genes related to the control of rice seed germination are still rare.…”

Quantitative Trait Loci Mapping of Mineral Element Contents in Brown Rice Using Backcross Inbred Lines Derived From Oryza longistaminata

“…Some QTLs related to mineral element accumulation identified from cultivated rice and common wild rice have been successfully applied in production ( Garcia-Oliveira et al., 2009 ; Nawaz et al., 2015 ). In this study, we focused on wild rice O. longistaminata , an Africa-derived important genetic resource that less favorable genes were explored ( Zhang et al., 2015 ; Fan et al., 2017 ; Jin et al., 2018 ; Fan et al., 2019 ). Here, we firstly identified 33 QTLs for content of six mineral elements from O. longistaminata .…”

“…The 127 BILs were subjected to whole-genome sequencing, and a high-quality bin map of ultrahigh-density SNPs was constructed based on the sequencing data (unpublished data). The DNA extraction, SNP screening, genotyping, bin map and genetic linkage map construction all adopt the previous reported methods ( Jin et al., 2018 ; Fan et al., 2019 ). A total of 2,432 bins were obtained for the subsequent QTL analysis.…”

Deciphering the Genetic Basis of Lodging Resistance in Wild Rice Oryza longistaminata

“…A total of 2,432 bin markers was used to construct the genetic linkage map covering the whole genome as described by Jin et al (2018). Inclusive composite interval mapping combined with additive mapping (ICIM-ADD) method was used to detect more precise lodging resistance QTLs (Meng et al, 2015).…”