QTL mapping of seedling tolerance to exposure to low temperature in the maize IBM RIL population

Goering, Raeann; Larsen, S.; Tan, Jia W.; Whelan, James; Makarevitch, Irina

doi:10.1371/journal.pone.0254437

Cited by 20 publications

(9 citation statements)

References 24 publications

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“…Low-temperature stress can seriously affect normal crop development, and quinoa shows different levels of sensitivity to low temperatures at different stages of reproduction. Low-temperature stress at the seedling stage generally results in weak seedlings, delaying reproduction [ 30 ], and in severe cases, it causes extensive seedling mortality, ultimately affecting quality and yield.…”

Section: Discussionmentioning

confidence: 99%

Transcriptomic and Metabolomic Analysis of the Response of Quinoa Seedlings to Low Temperatures

et al. 2022

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Quinoa, a cool-weather high-altitude crop, is susceptible to low-temperature stress throughout its reproductive phase. Herein, we performed broadly targeted metabolic profiling of quinoa seedlings to explore the metabolites’ dynamics in response to low-temperature stress and transcriptome analysis to determine the underlying genetic mechanisms. Two variants, namely, Dian Quinoa 2324 and Dian Quinoa 281, were exposed to temperatures of −2, 5, and 22 °C. A total of 794 metabolites were detected; 52,845 genes, including 6628 novel genes, were annotated using UPLC-MS/MS analysis and the Illumina HiSeq system. Combined with morphological indicators to resolve the mechanism underlying quinoa seedling response to low-temperature stress, the molecular mechanisms of quinoa changed considerably based on temperature exposure. Soluble sugars heavily accumulated in plants with cold damage and changes in regulatory networks under freeze damage, such as the upregulation of α-linolenic acid metabolism and a reduction in energy substrates, may explain the spatial patterns of biosynthesis and accumulation of these metabolites. Genes that are actively expressed during cold responses, as revealed by co-expression analyses, may be involved in the regulation thereof. These results provide insights into the metabolic factors in quinoa under low-temperature stress and provide a reference for the screening of quinoa varieties resistant to low temperature.

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Section: Discussionmentioning

confidence: 99%

Transcriptomic and Metabolomic Analysis of the Response of Quinoa Seedlings to Low Temperatures

et al. 2022

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“…Explorations of cold response at genotypic/species levels can further unveil the overall tolerance mechanism. For example, in maize [ 99 ], two widely used inbreds, i.e., B73 and Mo17 and 37 other inbreeds (grouped into stiff-stalk, non-stiff stalk and tropical clades) exhibited variable cold tolerance behaviour that was evaluated at seedling stages. Recombinant inbreed (B73 × Mo17) line (RIL) populations were developed (total of 97 RILs) to identify the QTLs responsible for the cold response at seedling stages.…”

Section: Membrane Transporters and Abiotic Stressesmentioning

confidence: 99%

The Role of Membrane Transporters in Plant Growth and Development, and Abiotic Stress Tolerance

Gill

Ahmar

Ali

et al. 2021

IJMS

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The proteins of membrane transporters (MTs) are embedded within membrane-bounded organelles and are the prime targets for improvements in the efficiency of water and nutrient transportation. Their function is to maintain cellular homeostasis by controlling ionic movements across cellular channels from roots to upper plant parts, xylem loading and remobilization of sugar molecules from photosynthesis tissues in the leaf (source) to roots, stem and seeds (sink) via phloem loading. The plant’s entire source-to-sink relationship is regulated by multiple transporting proteins in a highly sophisticated manner and driven based on different stages of plant growth and development (PG&D) and environmental changes. The MTs play a pivotal role in PG&D in terms of increased plant height, branches/tiller numbers, enhanced numbers, length and filled panicles per plant, seed yield and grain quality. Dynamic climatic changes disturbed ionic balance (salt, drought and heavy metals) and sugar supply (cold and heat stress) in plants. Due to poor selectivity, some of the MTs also uptake toxic elements in roots negatively impact PG&D and are later on also exported to upper parts where they deteriorate grain quality. As an adaptive strategy, in response to salt and heavy metals, plants activate plasma membranes and vacuolar membrane-localized MTs that export toxic elements into vacuole and also translocate in the root’s tips and shoot. However, in case of drought, cold and heat stresses, MTs increased water and sugar supplies to all organs. In this review, we mainly review recent literature from Arabidopsis, halophytes and major field crops such as rice, wheat, maize and oilseed rape in order to argue the global role of MTs in PG&D, and abiotic stress tolerance. We also discussed gene expression level changes and genomic variations within a species as well as within a family in response to developmental and environmental cues.

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“…Zhao et al [ 48 ] reported that the broad sense heritability (

) of Pn, Tr, Rubisco activity, and WUE were 88.9%, 69.7%, 68.8%, 86.8% in a maize F 4 population of 218 lines under well-watered and water-stress environments, respectively, while their genotype × environment interaction heritability (

) was 9.9%, 15.6%, 9.7%, and 1.5%, respectively. Goering et al [ 49 ] showed that the

of chlorophyll concentration, i.e., absorbance at 663 nm (Chl a) and 645 nm (Chl b) were 88.5% and 82.5% in a maize IBM recombinant Inbred Lines (RIL) population at 24 °C and 4 °C conditions, respectively. David et al [ 50 ] analyzed the genetic basis of SOD activity, CAT activity, POD activity, APX activity, root length, and root weight by QTL mapping in rice.…”

Section: Discussionmentioning

confidence: 99%

Understanding and Comprehensive Evaluation of Cold Resistance in the Seedlings of Multiple Maize Genotypes

Zhao

Niu

et al. 2022

Plants

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Maize is a cold-sensitive crop, and it exhibits severe retardation of growth and development when exposed to cold snaps during and right after seedling emergence. Although different agronomic, physiological, and molecular approaches have been tried to overcome the problems related to cold stress in recent years, the mechanisms causing cold resistance in maize are still unclear. Screening and breeding of varieties for cold resistance may be a sustainable option to boost maize production under low-temperature environments. Herein, seedlings of 39 different maize genotypes were treated under both 10 °C low temperature and 22 °C normal temperature conditions for 7 days, to assess the changes in seven growth parameters, two membrane characteristics, two reactive oxygen species (ROS) levels, and four antioxidant enzymes activities. The changes in ten photosynthetic performances, one osmotic substance accumulation, and three polyamines (PAs) metabolisms were also measured. Results indicated that significant differences among genotypes, temperature treatments, and their interactions were found in 29 studied traits, and cold–stressed seedlings were capable to enhance their cold resistance by maintaining high levels of membrane stability index (66.07%); antioxidant enzymes activities including the activity of superoxide dismutase (2.44 Unit g−1 protein), peroxidase (1.65 Unit g−1 protein), catalase (0.65 μM min−1 g−1 protein), and ascorbate peroxidase (5.45 μM min−1 g−1 protein); chlorophyll (Chl) content, i.e., Chl a (0.36 mg g−1 FW) and Chl b (0.40 mg g−1 FW); photosynthetic capacity such as net photosynthetic rate (5.52 μM m−2 s−1) and ribulose 1,5–biphosphate carboxylase activity (6.57 M m−2 s−1); PAs concentration, mainly putrescine (274.89 nM g−1 FW), spermidine (52.69 nM g−1 FW), and spermine (45.81 nM g−1 FW), particularly under extended cold stress. Importantly, 16 traits can be good indicators for screening of cold–resistant genotypes of maize. Gene expression analysis showed that GRMZM2G059991, GRMZM2G089982, GRMZM2G088212, GRMZM2G396553, GRMZM2G120578, and GRMZM2G396856 involved in antioxidant enzymes activity and PAs metabolism, and these genes may be used for genetic modification to improve maize cold resistance. Moreover, seven strong cold–resistant genotypes were identified, and they can be used as parents in maize breeding programs to develop new varieties.

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QTL mapping of seedling tolerance to exposure to low temperature in the maize IBM RIL population

Cited by 20 publications

References 24 publications

Transcriptomic and Metabolomic Analysis of the Response of Quinoa Seedlings to Low Temperatures

Transcriptomic and Metabolomic Analysis of the Response of Quinoa Seedlings to Low Temperatures

The Role of Membrane Transporters in Plant Growth and Development, and Abiotic Stress Tolerance

Understanding and Comprehensive Evaluation of Cold Resistance in the Seedlings of Multiple Maize Genotypes

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