QTL mapping of the pasting properties of wheat flour treated by papain digestion

Abstract

Protein hydrolysis is widely used in the food industry to improve food or industrial quality, and processing is carried out concurrently or subsequently to starch pasting. In this study, QTL mapping of the pasting properties of natural wheat flour (NWF) and papain‐treated flour (PTF) was performed in three environments. These analyses involved a set of 173 F9:10 recombinant inbred lines (RILs) derived from “Shannong01‐35 × Gaocheng9411” with a genetic linkage map consisting of 6248 molecular markers. The additive, epistatic, and epistatic × environment interaction effects of QTLs for pasting properties were analyzed. A total of 43 and 31 additive QTLs for pasting properties were detected using NWF and PTF, respectively. QPv1A.4‐1, QPv4B.4‐17, QTv1A.4‐1, QTv4B.4‐17, QSb4B.4‐17, QPt1A.4‐1, and QPt4B.4‐17 were identified using both NWF and PTF. In contrast, QPv6A.2‐128, QTv6A.2‐128, QFv6A.2‐75, and QBd6A.2‐128 were only identified in wheat flour; thus, these QTLs are related to the papain digestion site. Five and eight pairs of epistatic interaction QTLs were identified in NWF and PTF, respectively. QPv1A.4‐1/QPv4B.4‐17 and QTv1A.4‐1/QTv4B.4‐17 were identified in NWF and PTF, whereas, QInA1A.4‐1 and QInA4B.4‐17 were only detected in PTF. Two and three pairs of epistatic × environment interactions were detected in NWF and PTF, respectively. In the present study, two important QTL clusters (≥3 QTLs) were detected from NWF, and three clusters were detected from PTF. The results of the comprehensive evaluation of protein hydrolysis and starch pasting provide valuable information to facilitate the improvement of wheat flour quality and promote the molecular breeding of wheat.

Identification of solvent retention capacity quantitative trait loci by combined linkage and association mapping in wheat (Triticum aestivum L.)

“…Furthermore, a major QTL for SC‐SRC, QSc4B.4‐13 , was also located within this colocated chromosomal interval associated with IACX557 (28,954,114). Previous studies have reported QTLs for kernel protein (Liu, Liu et al., 2017), steamed bread textural properties (Liu, An et al., 2017), and flour pasting properties (Wang et al., 2016) flanking IACX557 . Interestingly, IACX557 is located near known genes such as LoxA/B for yellow pigment content (27.5 Mb), Rht‐B1 for plant height (∼30.861 Mb), and FLO6 for starch synthesis (21.937 Mb).…”

Identification and Validation of Stable Quantitative Trait Loci for SDS-Sedimentation Volume in Common Wheat (Triticum aestivum L.)

“…QSsv.cau-4B.1 and QSsv.cau-4D was located next to the dwarf genes Rht-B1 and Rht-D1, respectively. Previous studies have also revealed QTL-enrichment areas near Rht-B1 and Rht-D1, which are associated with kernel size, kernel hardness, kernel protein, pasting properties, and mixing properties (Shanhong et al, 2001;Li et al, 2006;Wang et al, 2012Wang et al, , 2017Patil et al, 2013;Zhang et al, 2013;Jin et al, 2016;Liu et al, 2017b).…”

Wheat end-use quality: State of art, genetics, genomics-assisted improvement, future challenges, and opportunities

“…A combination of RFLP, SSR, and Amplified Fragment Length Polymorphism (AFLP) was also used to increase the marker density in maps ( Table 1 ) ( Sourdille et al, 2003 ; Mohler et al, 2014 ). With the advancement in sequencing technologies, the use of SNP has been prioritized since they are abundant and uniformly distributed in a genome providing high genomic resolution ( Gupta et al, 2008 ; Kang et al, 2016 ; Wang et al, 2017 ; Naraghi et al, 2019 ; Guo Y. et al, 2020 ). The availability of high throughput sequencing platform for SNPs makes it a preferred genotyping method in general.…”