QTLs for Fusarium head blight response in a wheat DH population of Wangshuibai/Alondra‘s’

Abstract

A doubled haploid (DH) wheat population derived from the cross Wangshuibai/Alondra's' was developed through chromosome doubling of haploids generated by anther culture of hybrids. Fusarium head blight (FHB) was evaluated for three years from 2001 to 2003 in Jianyang, Fujian Province, China, where epidemics of FHB have been consistently severe. After 307 pairs of simple sequence repeat (SSR) primers were screened, 110 pairs were polymorphic between Wangshuibai and Alondra's', and used to construct a genetic linkage map for detection of quantitative trait loci (QTLs). A stable QTL for low FHB severity was detected on chromosomes 3B over all three years, and QTLs on chromosomes 5B, 2D, and 7A were detected over two years. Additional QTLs on chromosomes 3A, 3D, 4B, 5A, 5D, 6B and 7B showed marginal significance in only one year. Six QTLs were detected when phenotypic data from three years were combined. In addition, significant additive-by-additive epistasis was detected for a QTL on 6A although its additive effect was not significant. Additive effects (A) and additive-by-additive epistasis (AA) explained a major portion of the phenotypic variation (76.5%) for FHB response. Xgwm533-3B and Xgwm335-5B were the closest markers to QTLs, and have potential to be used as selectable markers for marker-assisted selection (MAS) in wheat breeding programs.

Quantitative Trait Loci Analysis of Novel Fusarium Head Blight Resistance in Tokai 66

“…A change in the dominance of F. graminearum isolates across the two years might have also lead to variability in DON content in the RILs in 2006 and 2007. DArT markers detected one stable QTL for severity. This stable 5B QTL should be in a similar region where 5B QTL from Wangshuibai, a Chinese source of resistance, was reported (Akbari et al, 2006;Jia et al, 2005;Somers et al, 2004). In a haplotype study, Liu and Anderson (2003) reported that Sumai3 and Tokai66 contained the same 3BS allele when genotyped with the XBARC133 marker, whereas Wangshuibai did not contain any Sumai3 allele.…”

Cereal resistance to Fusarium head blight and possibilities of its improvement through breeding

“…del Blanco et al 2003;, other cultivars with relatively high FHB resistance come from this region, e.g. Chinese cultivars Ning 7840, Ning 894037 (Shen et al 2003a;Zhou et al 2003), Wangshuibai Jia et al 2005;Mardi et al 2005;Lin et al 2004Lin et al , 2006Ma et al 2006), Wuhan, Japanese cultivars Nobeokabozu and Nyu Bai (Nyubai) or Korean cultivar Chokwang (Yang et al 2005). Sumai 3 is a highly FHB resistant Chinese spring wheat cultivar which was derived from a cross between Italian wheat Funo and Chinese landrace line Taiwan Xiaomai Wheat.…”

Gene expression analysis of four WIR1-like genes in floret tissues of European winter wheat after challenge with G. zeae

“…No QTL for FHB resistance was identified on chromosome 5D in this population (Schmolke et al 2005). However, Holzapfel et al (2008) and Jia et al (2005) described QTL for FHB resistance in the same chromosomal region of 5DS in the populations Solitär/Travix and Wangshuibai/Alondra-S. Douchkov et al (2011) mapped HvWIR1a, a barley homologue of wheat WIR1a and WIR1b genes on barley chromosome 5HS. As barley chromosome 5H is homoeologous to the wheat group 5 chromosomes, the results of the mapping in wheat and barley were consistent.…”