Multiple-target localization is extensively applied in wireless connected networks. However, sensor location uncertainty is known to degrade significantly the target localization accuracy. Fortunately, calibration emitters such as unmanned aerial vehicles (UAV) with known location can be used to reduce the loss in localization accuracy due to sensor location errors. This paper is devoted to the use of UAV calibration emitters for time differences of arrival (TDOA) and frequency differences of arrival (FDOA) positioning of multiple targets. The study starts with deriving the Cramér-Rao bound (CRB) for TDOA/FDOA-based target location estimate when several UAV calibration signals are available. Subsequently, the paper presents an iterative constrained weighted least squares (ICWLS) estimator for multiple-target joint localization using TDOA/FDOA measurements from both target sources and UAV calibration emitters. The newly proposed method consists of two stages. In the first phase, the sensor locations are refined based on the calibration measurements as well as the prior knowledge of sensor locations. The second step provides the estimate of multiple-target locations by combining the measurements of target signals as well as the estimated values in the first phase. An efficient ICWLS algorithm is presented at each stage. Both the two algorithms are implemented by using matrix singular value decomposition (SVD), which is able to provide a closed-form solution and update the weighting matrix at every iteration. Finally, the convergence behavior and estimation mean-square-error (MSE) of the new estimator are deduced. Both theoretical analysis and simulation results show that the developed method can improve the TDOA/FDOA localization accuracy obviously with the help of UAV calibration emitters. INDEX TERMS Target localization, unmanned aerial vehicle (UAV), time difference of arrival (TDOA), frequency difference of arrival (FDOA), multiple targets, calibration emitters, constrained weighted least squares (CWLS), Cramér-Rao bound (CRB), singular value decomposition (SVD).