Quadruplex real-time quantitative PCR assay for the detection of pathogens related to late-onset ventilator-associated pneumonia

Comprehensive Molecular Testing for Respiratory Pathogens in Community-Acquired Pneumonia

“…Quantitative molecular assays for typical respiratory bacteria have been used previously in patients with pneumonia, but these assays lack a number of key targets, use targets that have been shown to lack specificity, or may be only semiquantitative [16][17][18][19][20][21][22][23][24][25][26][27][28][29][30][31][32]. However, our group recently developed a suitable assay that is capable of quantifying bacterial loads of 8 typical bacterial pathogens from lower respiratory tract (LRT) specimens [33].…”

Rapid quantification of Staphylococcus aureus from endotracheal aspirates of ventilated patients: a proof-of-concept study

“…Previous studies have already shown the use and interest of molecular tools to provide shorter microbiological evidence, but they mainly focused on BAL due to their easier analytical management (Apfalter et al, 2005;Rios-Licea et al, 2010). In this proof-ofconcept study, we report the evaluation of a semiautomated molecular method to rapidly quantify Staphylococcus aureus, the most involved microorganisms in VAP (Restrepo et al, 2013), directly from ETA samples.…”

Design and construction of a single tube, quantitative endpoint, LATE-PCR multiplex assay for ventilator-associated pneumonia

“…The goal of this study was to consistently distinguish all of these pathogens in a single closed-tube assay when the number of genomes present initially was varied over five orders of magnitude, 10 1 -to-10 5 . No other VAP PCR assays either in the literature or commercially marketed attempt to detect these pathogens, antibiotic resistance genes, or starting genomic DNA copy numbers as low as 10 (Apfalter et al 2005;Rios-Licea et al 2010).…”