Quadruply-labeled serum albumin as a biodegradable nanosensor for simultaneous fluorescence imaging of intracellular pH values, oxygen and temperature

Zhang, Xiao-Ai; Zhang, Wei; Wang, Qi; Wang, Junli; Ren, Guodong; Wang, Xudong

doi:10.1007/s00604-019-3674-4

Cited by 14 publications

(14 citation statements)

References 35 publications

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“…A referenced triple sensor for intracellular measurement of temperature, pH, and oxygen concentration was presented by the group of Wang. 986 Bovine serum albumin (BSA) is selected as the scaffold (support) to which four fluorophores were linked. The following luminophores were used: (a) fluorescein as a probe for pH values, (b) a platinum(II) porphyrin complex for oxygen, (c) a europium(III) clathrate complex for temperature, and (d) rhodamine B as a reference dye.…”

Section: Luminescent Sensorsmentioning

confidence: 99%

Optical Sensing and Imaging of pH Values: Spectroscopies, Materials, and Applications

2020

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This is the first comprehensive review on methods and materials for use in optical sensing of pH values and on applications of such sensors. The Review starts with an introduction that contains subsections on the definition of the pH value, a brief look back on optical methods for sensing of pH, on the effects of ionic strength on pH values and p K a values, on the selectivity, sensitivity, precision, dynamic ranges, and temperature dependence of such sensors. Commonly used optical sensing schemes are covered in a next main chapter, with subsections on methods based on absorptiometry, reflectometry, luminescence, refractive index, surface plasmon resonance, photonic crystals, turbidity, mechanical displacement, interferometry, and solvatochromism. This is followed by sections on absorptiometric and luminescent molecular probes for use pH in sensors. Further large sections cover polymeric hosts and supports, and methods for immobilization of indicator dyes. Further and more specific sections summarize the state of the art in materials with dual functionality (indicator and host), nanomaterials, sensors based on upconversion and 2-photon absorption, multiparameter sensors, imaging, and sensors for extreme pH values. A chapter on the many sensing formats has subsections on planar, fiber optic, evanescent wave, refractive index, surface plasmon resonance and holography based sensor designs, and on distributed sensing. Another section summarizes selected applications in areas, such as medicine, biology, oceanography, bioprocess monitoring, corrosion studies, on the use of pH sensors as transducers in biosensors and chemical sensors, and their integration into flow-injection analyzers, microfluidic devices, and lab-on-a-chip systems. An extra section is devoted to current challenges, with subsections on challenges of general nature and those of specific nature. A concluding section gives an outlook on potential future trends and perspectives.

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Section: Luminescent Sensorsmentioning

confidence: 99%

Optical Sensing and Imaging of pH Values: Spectroscopies, Materials, and Applications

2020

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“…To investigate the transience and dynamic nature of the pentamers formed by domain P, which polypeptides containing domain P can be dynamically transferred from one nanogel to another one, FRET method was selected to test their specific properties. FITC and RBITC were widely used as fluorescent markers for polypeptides labeling through the reaction between isothiocyanate in dyes and primary amines in polypeptides as the donor and receptor for FRET system [31]. UV–Vis absorption spectra of dyes and conjugates were measured, and the results are shown in Figure 1A.…”

Section: Resultsmentioning

confidence: 99%

A capillary electrophoresis strategy to sensitively detect dynamic properties of coiled coil polypeptides

Yang

Zhao

et al. 2020

J of Separation Science

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K E Y W O R D Scapillary electrophoresis, coiled coil polypeptides, dynamic properties, Förster resonance energy transfer Article Related Abbreviations: A, leucine zipper domain (designated A); C 10 , (AGAGAGPEG) 10 (designated C 10 ); FITC, fluorescein isothiocyanate; FRET, Förster resonance energy transfer; Ni-NTA, nickel-nitrilotriacetic acid; P, cartilage oligomeric matrix protein (designated P); RBITC, rhodamine B isothiocyanate.

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“…[74] Finally, it is worth mentioning that the use of multiple emissions from the same emitter allows one to measure different parameters (e.g., temperature, pH, ROS) in the same assay using a single sensor. [83][84][85][86][87][88][89] In this same line, distinct compounds (e.g., different ROS) can be detected using a fluorescent sensor that emit at a different color in the presence of different analytes. [90][91][92] Moreover, the emitter can be designed to present emission bands in the visible and infrared ranges to be used for both in vitro and in vivo fluorescence molecular detection.…”

Section: Sensing Techniquesmentioning

confidence: 99%

Multichannel Fluorescence Microscopy: Advantages of Going beyond a Single Emission

Rodríguez‐Sevilla

Thompson

Jaque

2022

Advanced NanoBiomed Research

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Fluorescent microscopy has enabled the study of intracellular processes and revealed the most intricate details of the subcellular structure. This has benefitted not only the basic biological science, but also has had an impact in numerous biomedical applications. Basic fluorescent sensing techniques use the change in the absolute emission of a fluorescent sensor. This entails some disadvantages as the signal might be influenced by factors not directly related to the process under study (e.g., fluctuations in the excitation source). To overcome these drawbacks, one can use multiple emissions of a single or various fluorophores. There are numerous examples of multichannel fluorescence microscopy techniques that have given rise to numerous ratiometric methods and multiplexing assays. Herein, how the use of multiple emission channels has impacted fluorescence microscopy in terms of speed, sensitivity, and resolution is reviewed. Using recent examples, how the easy implementation of multichannel detection can overcome current limitations of the main used fluorescence techniques and promote the development of novel microscopy methods is shown.

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Quadruply-labeled serum albumin as a biodegradable nanosensor for simultaneous fluorescence imaging of intracellular pH values, oxygen and temperature

Cited by 14 publications

References 35 publications

Optical Sensing and Imaging of pH Values: Spectroscopies, Materials, and Applications

Optical Sensing and Imaging of pH Values: Spectroscopies, Materials, and Applications

A capillary electrophoresis strategy to sensitively detect dynamic properties of coiled coil polypeptides

Multichannel Fluorescence Microscopy: Advantages of Going beyond a Single Emission

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