Altitudes, ranging between 100 m and 900 m are the most suitable for growing passion fruit and the studies on the distinct geographical locations suggest the expression of different genotypes under the influence of adequate environmental conditions. The objective of this study was to evaluate the physiological quality of the seeds of Passiflora spp., cultivated at three different altitudes in the state of Espírito Santo, Brazil. Before conducting the physiological tests for seed quality, pre-tests were performed on the physically scarified, gibberellic acid-treated, and intact seeds of the yellow passion fruit (Passiflora edulis Sims f. flavicarpa Degener), the purple passion fruit (Passiflora edulis Sims), and the sweet passion fruit (Passiflora alata Curtis), collected from three different altitudes (high, medium, and low), in order to overcome dormancy. The higher values of germination and vigor were observed after the pre-germination treatments of the intact seeds of the yellow passion fruit, the scarified seeds of the purple passion fruit, and the gibberellic acid-treated seeds of the sweet passion fruit. The seeds with the highest values of germination and vigor were selected for the accelerated aging test with a saturated salt solution at 43 ºC for 72 h and the controlled deterioration test with 25% of moisture at 45 ºC for 24 h, respectively. Using these tests, it was possible to differentiate the species of passion fruit, collected from different altitudes. The seeds of the yellow passion fruit and the seeds, collected from higher altitudes presented higher physiological quality in this study.