Qualifying P-glycoprotein in drug-resistant ovarian cancer cells: a dual-mode aptamer probe approach

Pang, Chaobin; Xu, Heng; Xu, Jichao; Zhang, Lei; Wang, Jinhua; Jing, Su

doi:10.1039/d4an00803k

Analyst

2024

DOI: 10.1039/d4an00803k

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Qualifying P-glycoprotein in drug-resistant ovarian cancer cells: a dual-mode aptamer probe approach

Chaobin Pang,

Heng Xu,

Jichao Xu

et al.

Abstract: Our dual-mode FI and FP aptamer probe detects P-glycoprotein in drug-resistant ovarian cancer cells, demonstrating high specificity and sensitivity with detection limits of 1.13 nM and 40 cells per mL, promising improved diagnosis strategies.

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Development of an Automated Capture-SELEX Device for Efficient Screening of β-Conglycinin Aptamer

Zhang,

Ning

et al. 2024

J. Agric. Food Chem.

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β-Conglycinin is the main allergen present in soybeans, and it is causing wide concern due to its notable allergenicity, heat, and digestive enzyme resistance. Screening for aptamers that both recognize β-conglycinin and inhibit the allergic reactions that it triggers is necessary. Conventional aptamer screening is labor-intensive, requires skilled personnel, and has limited reproducibility. To address these limitations, an automated device was developed to enhance the efficiency of aptamer selection in Capture-SELEX. The device achieves highly integrated, reproducible, and accurate contamination control. Using this device, a high-affinity and specific aptamer, β-5, was selected with a K d = 18.24 ± 2.42 nM for β-conglycinin, as confirmed by isothermal titration calorimetry and fluorescence polarization. Thermodynamic analysis revealed that enthalpy-driven binding and docking simulations clarified the recognition mechanism. Overall, this automated device enables high-efficiency aptamer generation for certain targets, with aptamer β-5 expected to play a vital role in the detection of β-conglycinin and the targeted inhibition of its allergic reaction.

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Development of an Automated Capture-SELEX Device for Efficient Screening of β-Conglycinin Aptamer

Zhang,

Ning

et al. 2024

J. Agric. Food Chem.

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show abstract

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Qualifying P-glycoprotein in drug-resistant ovarian cancer cells: a dual-mode aptamer probe approach

Abstract: Our dual-mode FI and FP aptamer probe detects P-glycoprotein in drug-resistant ovarian cancer cells, demonstrating high specificity and sensitivity with detection limits of 1.13 nM and 40 cells per mL, promising improved diagnosis strategies.

Cited by 1 publication

References 37 publications

Development of an Automated Capture-SELEX Device for Efficient Screening of β-Conglycinin Aptamer

Development of an Automated Capture-SELEX Device for Efficient Screening of β-Conglycinin Aptamer

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