Qualitative Analysis of Lipid Peroxidation in Plants under Multiple Stress  Through Schiff’s Reagent: A Histochemical Approach

Awasthi, Jay Prakash; Saha, Bedabrata; Chowardhara, Bhaben; Devi, Sanjenbam Sanjibia; Borgohain, Pankaj; Panda, Sanjib Kumar

doi:10.21769/bioprotoc.2807

Cited by 55 publications

(16 citation statements)

References 6 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Lipids are primary targets of ROS attack and the free radicals oxidation of polyunsaturated fatty acids is called LP [ 55 ]. As a byproduct of LP, malonaldehyde (MDA) has been largely used as an important indicator to evaluate the extent of damaging effects caused by ROS and oxidative stress combination on membrane lipids to reduce membrane stability [ 56 ]. The MDA content was tremendously increased by 36% in ginseng root plantlets [ 50 ], 39% in maize [ 42 ], 47% in peanut [ 48 ], 70% in chickpea [ 35 ], 131% in oat [ 44 ], 153% in mung bean [ 36 ], and 300% in rice seedlings [ 57 ], indicating an severe damage to cell membrane integrity and/or membrane permeability during salinity exposure [ 58 ].…”

Section: Adverse Effects Of Salinity On Plantsmentioning

confidence: 99%

Roles of Plant Growth-Promoting Rhizobacteria (PGPR) in Stimulating Salinity Stress Defense in Plants: A Review

Ha-Tran

Nguyen

Hung

et al. 2021

IJMS

149

View full text Add to dashboard Cite

To date, soil salinity becomes a huge obstacle for food production worldwide since salt stress is one of the major factors limiting agricultural productivity. It is estimated that a significant loss of crops (20–50%) would be due to drought and salinity. To embark upon this harsh situation, numerous strategies such as plant breeding, plant genetic engineering, and a large variety of agricultural practices including the applications of plant growth-promoting rhizobacteria (PGPR) and seed biopriming technique have been developed to improve plant defense system against salt stress, resulting in higher crop yields to meet human’s increasing food demand in the future. In the present review, we update and discuss the advantageous roles of beneficial PGPR as green bioinoculants in mitigating the burden of high saline conditions on morphological parameters and on physio-biochemical attributes of plant crops via diverse mechanisms. In addition, the applications of PGPR as a useful tool in seed biopriming technique are also updated and discussed since this approach exhibits promising potentials in improving seed vigor, rapid seed germination, and seedling growth uniformity. Furthermore, the controversial findings regarding the fluctuation of antioxidants and osmolytes in PGPR-treated plants are also pointed out and discussed.

show abstract

Section: Adverse Effects Of Salinity On Plantsmentioning

confidence: 99%

Roles of Plant Growth-Promoting Rhizobacteria (PGPR) in Stimulating Salinity Stress Defense in Plants: A Review

Ha-Tran

Nguyen

Hung

et al. 2021

IJMS

149

View full text Add to dashboard Cite

show abstract

“…Aldehyde produced as result of lipid peroxidation was visualized using the Schiff base assay (Aswathi et al, 2018 ). Excised leaf tissues were immediate immersed into the test tube containing 5 ml of 10% (v/v) Schiff's reagent solution.…”

Section: Experiments 1: Stress Imposition At Seedling Stage In Contrasmentioning

confidence: 99%

Induction of Acquired Tolerance Through Gradual Progression of Drought Is the Key for Maintenance of Spikelet Fertility and Yield in Rice Under Semi-irrigated Aerobic Conditions

et al. 2021

View full text Add to dashboard Cite

Plants have evolved several adaptive mechanisms to cope with water-limited conditions. While most of them are through constitutive traits, certain “acquired tolerance” traits also provide significant improvement in drought adaptation. Most abiotic stresses, especially drought, show a gradual progression of stress and hence provide an opportunity to upregulate specific protective mechanisms collectively referred to as “acquired tolerance” traits. Here, we demonstrate a significant genetic variability in acquired tolerance traits among rice germplasm accessions after standardizing a novel gradual stress progress protocol. Two contrasting genotypes, BPT 5204 (drought susceptible) and AC 39000 (tolerant), were used to standardize methodology for capturing acquired tolerance traits at seedling phase. Seedlings exposed to gradual progression of stress showed higher recovery with low free radical accumulation in both the genotypes compared to rapid stress. Further, the gradual stress progression protocol was used to examine the role of acquired tolerance at flowering phase using a set of 17 diverse rice genotypes. Significant diversity in free radical production and scavenging was observed among these genotypes. Association of these parameters with yield attributes showed that genotypes that managed free radical levels in cells were able to maintain high spikelet fertility and hence yield under stress. This study, besides emphasizing the importance of acquired tolerance, explains a high throughput phenotyping approach that significantly overcomes methodological constraints in assessing genetic variability in this important drought adaptive mechanism.

show abstract

“…Then, the samples were incubated in a growth chamber for 48 h and the chlorophyll was removed with ethanol (95%). The superoxide anion stains dark blue (Awasthi et al, 2018).…”

Section: Histochemical Stainingmentioning

confidence: 99%

ASR Enhances Environmental Stress Tolerance and Improves Grain Yield by Modulating Stomatal Closure in Rice

et al. 2020

View full text Add to dashboard Cite

Abscisic acid-, stress-, and ripening-induced (ASR) genes are involved in responding to abiotic stresses, but their precise roles in enhancing grain yield under stress conditions remain to be determined. We cloned a rice (Oryza sativa) ASR gene, OsASR1, and characterized its function in rice plants. OsASR1 expression was induced by abscisic acid (ABA), salt, and drought treatments. Transgenic rice plants overexpressing OsASR1 displayed improved water regulation under salt and drought stresses, which was associated with osmolyte accumulation, improved modulation of stomatal closure, and reduced transpiration rates. OsASR1-overexpressing plants were hypersensitive to exogenous ABA and accumulated higher endogenous ABA levels under salt and drought stresses, indicating that OsASR1 is a positive regulator of the ABA signaling pathway. The growth of OsASR1-overexpressing plants was superior to that of wild-type (WT) plants under paddy field conditions when irrigation was withheld, likely due to improved modulation of stomatal closure via modified ABA signaling. The transgenic plants had higher grain yields than WT plants for four consecutive generations. We conclude that OsASR1 has a crucial role in ABA-mediated regulation of stomatal closure to conserve water under salt-and drought-stress conditions, and OsASR1 overexpression can enhance salinity and drought tolerance, resulting in improved crop yields.

show abstract

Qualitative Analysis of Lipid Peroxidation in Plants under Multiple Stress Through Schiff’s Reagent: A Histochemical Approach

Cited by 55 publications

References 6 publications

Roles of Plant Growth-Promoting Rhizobacteria (PGPR) in Stimulating Salinity Stress Defense in Plants: A Review

Roles of Plant Growth-Promoting Rhizobacteria (PGPR) in Stimulating Salinity Stress Defense in Plants: A Review

Induction of Acquired Tolerance Through Gradual Progression of Drought Is the Key for Maintenance of Spikelet Fertility and Yield in Rice Under Semi-irrigated Aerobic Conditions

ASR Enhances Environmental Stress Tolerance and Improves Grain Yield by Modulating Stomatal Closure in Rice

Contact Info

Product

Resources

About