Qualitative and quantitative analyses of bioactive compounds from ex vitro Chamaenerion angustifolium (L.) (Epilobium augustifolium) herb in different harvest times

Gryszczyńska, Agnieszka; Dreger, Mariola; Piasecka, Anna; Kachlicki, P.; Witaszak, Natalia; Sawikowska, Aneta; Ożarowski, Marcin; Opala, Bogna; Łowicki, Zdzisław; Pietrowiak, Aurelia; Magdalena, Miklaś; Mikołajczak, Przemysław Ł.; Wielgus, Karolina

doi:10.1016/j.indcrop.2018.06.010

Cited by 25 publications

(25 citation statements)

References 33 publications

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“…Phytochemical studies on E. angustifolium resulted in the identification of about 250 different metabolites, of which about 170 substances have been found for the first time in this plant in the last six years [2,3,8,[42][43][44][45][46][47][48][49][50] (table 1). Polyphenols are the most abundant and are represented by flavonoids, phenolic acids, and hydrolysable tannins (ellagitannins).…”

Section: Chemical Compositionmentioning

confidence: 99%

“…Quercetin-3-O-glucuronide is the most dominant and characteristic flavonoid of E. angustifolium, contrary to myricetin-3-O-rhamnoside (myricitrin), which is the main flavonoid of the other Epilobium species [51]. Recently, flavonol glycoconjugates acylated with hydroxycinnamic acids were also detected [50].…”

Section: Flavonoidsmentioning

confidence: 99%

“…Phenolic acids and their derivatives are one of the dominant groups of phenolics found in E. angustifolium, and are important contributors to the antioxidant [48] and therapeutic potential against BPH of this species [49]. A great variety of these compounds, including 39 substances (e.g., caffeic, ellagic, ferulic, gallic, protocatechuic acids and caffeoylquinic acid isomers), has been found in the fireweed [8,43,[48][49][50].…”

Section: Phenolic Acidsmentioning

confidence: 99%

“…Oenothein B is a principal bioactive compound and the most abundant ellagitannin, whose content ranges up to 50% of the total mass of oligomeric ellagitannins in the extract [2]. Depending on the plant origin and harvest time, oenothein B concentration in the raw material varies from 2 to 4.5% [50,52,53]. This compound has been considered to be the main contributor to the anti-androgenic, antiproliferative and anticancer, antioxidant, anti-inflammatory as well as antimicrobial effects of E. angustifolium extracts [4].…”

Section: Hydrolysable Tanninsmentioning

confidence: 99%

“…Besides polyphenols, the aerial parts of E. angustifolium contain a lipophilic fraction rich in steroids, terpenoids and fatty acids. The presence of campesterol, cholesterol, stigmasterol as well as β-sitosterol and its derivatives was reported [49,50,[54][55][56]. Fatty acids (41 identified compounds) as well as triterpenes (e.g., oleanolic, pomolic and ursolic acids) are the main components of the lipophilic fraction of E. angustifolium extracts [42].…”

Section: Steroids Terpenoids and Fatty Acidsmentioning

confidence: 99%

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Fireweed (Epilobium angustifolium L.): botany, phytochemistry and traditional uses. A review

et al. 2019

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Fireweed (Epilobium angustifolium L., Onagraceae) is one of important medicinal plants used especially in the treatment of urogenital disorders, including benign prostatic hyperplasia (BPH) and prostatitis. The therapeutic effects of E. angustifolium extracts comprise antiproliferative, anti-inflammatory, immunomodulatory, antioxidant, and also antimicrobial activities. The aim of the present review was to provide the information on the botany, phytochemistry and traditional uses of E. angustifolium. This plant is a widespread circumboreal species of North America and Eurasia, tolerant in terms of habitat conditions, and often occupying man-made open habitats. Phytochemical studies on E. angustifolium resulted in the identification of about 250 different metabolites, including about 170 substances found for the first time in this plant in the last six years (2014)(2015)(2016)(2017)(2018)(2019). Fireweed has an abundance of polyphenolic compounds, particularly ellagitannins. Oenothein B and quercetin-3-O-glucuronide are proposed as markers for the identification and standardization of the plant raw material. E. angustifolium exhibits significant phytochemical variability in relation to the geographical origin, plant part and time of harvest/vegetation phase. Survey of the ethnobotanical literature showed that the above-mentioned species has been widely used not only as a medicinal, but also as an edible, honey and decorative plant. BOTANY TaxonomyEpilobium (Chamerion) angustifolium is classified to the Onagraceae family, which consists of 22 genera and approximately 650 species. The genus Chamerion (Raf.) Raf. ex Holub (syn. Chamaenerion Ség.) with eight species restricted to the northern hemisphere (mainly in Eurasia) belongs to the Onagroideae subfamily and Epilobieae tribe [18]. Relatively often, it is included in Epilobium L., which is the largest genus in the Onagraceae [19][20][21][22]. However, Chamerion clearly differs from Epilobium in terms of flower structure, leaf arrangement and other features [18,[23][24][25]. Molecular data also show that Chamerion is a separate monophyletic group of plants [26][27][28]. SynonymsThe Plant List [22] provides more than 40 synonyms of the species described, with three of these being the most commonly used: Epilobium angustifolium L., Chamerion angustifolium (L.) Holub and Chamaenerion angustifolium (L.) Scop. According to Holub [23], Chamerion should be considered the correct Latin name of this genus, while Chamaenerion is an illegitimate name. On the other hand, Sennikov [25] recently presented arguments for the restoration of the older name Chamaenerion. However, in Recently, the number of publications concerning the above-mentioned species has increased significantly. Screening of the Scopus database with the keywords of 'Epilobium/Chamerion/Chamaenerion angustifolium' found 416 document results, including 80 articles published in the years of 2014-2019. A new detailed summary is required not only for phytochemistry and pharmacology of E. angustifolium, but also in ...

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Section: Chemical Compositionmentioning

confidence: 99%

Section: Flavonoidsmentioning

confidence: 99%

Section: Phenolic Acidsmentioning

confidence: 99%

Section: Hydrolysable Tanninsmentioning

confidence: 99%

Section: Steroids Terpenoids and Fatty Acidsmentioning

confidence: 99%

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Fireweed (Epilobium angustifolium L.): botany, phytochemistry and traditional uses. A review

et al. 2019

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Chemical characterization of the anti-inflammatory activity fraction of Epilobium angustifolium

Lin

Wang

Zhou

et al. 2021

Eur Food Res Technol

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Micropropagation and HPLC-DAD, UPLC MS/MS analysis of oenothein B and phenolic acids in shoot cultures and in regenerated plants of fireweed (Chamerion angustifolium (L.) Holub)

Dreger

Gryszczyńska

Szalata

et al. 2020

Plant Cell Tiss Organ Cult

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In this study, a micropropagation protocol using nodal explants from in vitro grown plants of Chamerion angustifolium (L.) Holub was developed and analysis of oenothein B and selected phenolic acids in shoot cultures was performed for the first time. For shoot induction and multiplication Murashige and Skoog’s (MS) basal medium supplemented with 2-isopentenyladenine (2iP), zeatin (Z) and 6-benzyloaminopurine (BAP) was used. 2iP was the most responsive in terms of promoting shoots per explant with the maximum (6.57 ± 1.14) recorded at a concentration of 2.0 mg L−1 after 6 weeks of culture. After two subcultures the multiplication rate was increased up to 19 shoots per explant on medium with 2iP (1.0 mg L−1). To prevent tissue browning, ascorbic acid and casein hydrolysate were added to the induction medium, resulting in a reduction of browning by 30%. The rooted plantlets were successfully transferred to soil and acclimatized with 97% frequency. Quantitative and qualitative assessments of oenothein B and phenolic acid contents in in vitro regenerated shoots as well as in ex vitro plants were performed using high-performance liquid chromatography with a diode-array detector (HPLC-DAD) and ultra-performance liquid chromatography–tandem mass spectrometry (UPLC MS/MS) methods. Oenothein B (1.62‒4.55 g 100 g−1 DW), ellagic acid, gallic and caffeic acids were identified in in vitro regenerated plants. The results of this study confirm that the oenothein B-producing plantlets can be obtained using the micropropagation method with axillary shoots being a valuable source of oenothein B and phenolic acids.

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Qualitative and quantitative analyses of bioactive compounds from ex vitro Chamaenerion angustifolium (L.) (Epilobium augustifolium) herb in different harvest times

Cited by 25 publications

References 33 publications

Fireweed (Epilobium angustifolium L.): botany, phytochemistry and traditional uses. A review

Fireweed (Epilobium angustifolium L.): botany, phytochemistry and traditional uses. A review

Chemical characterization of the anti-inflammatory activity fraction of Epilobium angustifolium

Micropropagation and HPLC-DAD, UPLC MS/MS analysis of oenothein B and phenolic acids in shoot cultures and in regenerated plants of fireweed (Chamerion angustifolium (L.) Holub)

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