Qualitative and quantitative analysis of a group of volatile organic compounds in biological samples by HS-GC/FID: application in practical cases

Monteiro, Carla; Franco, João Miguel; Proença, Paula; Ajenjo, Antonio Castañera; Claro, Alda; Vieira, Duarte Nuno; Côrte-Real, F.

doi:10.1016/j.forsciint.2014.07.016

Cited by 17 publications

(12 citation statements)

References 26 publications

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“…The separation of toluene metabolites from biological fluid can easily be achieved by gas chromatography (GC) with headspace method [21,22]. However, gas chromatography-mass spectrometry (GC-MS) appears very suitable for the identification of toluene and their metabolites [23][24][25][26][27].…”

Section: Toluene Exposure Effects and Analysismentioning

confidence: 99%

“…Selected examples of conditions for sample pretreatment and GC separation of toluene metabolite in biological sample are listed in Table 4. Head space method can achieve good separation of volatile solvents from the biological matrix [22]. In addition, the determination of toluene and its metabolites was not a problem as the GC peaks were well separated [21].…”

Section: Analysis Of Vocs By Gas Chromatographymentioning

confidence: 99%

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Chromatographic Determination of Toluene and Its Metabolites in Urine for Toluene Exposure – A Review

Zinalibdin¹,

Yacob²,

Sanagi

et al. 2016

MJAS

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The determinations of toluene and their metabolites in biological samples such as urine and blood allow the estimation of the degree of exposure to this chemical. Chromatographic methods and preliminary methods are now universally employed for this purpose. Preliminary color test methods are well established for qualitative determination of toluene and its metabolites. Mobile test kits using color test methods are a vast tool for screening urine samples but chromatographic methods are still needed for confirmation and quantitative analysis. Gas chromatography (GC) methods are well-adapted for the determination of toluene metabolite in urine, but these methods often require several pretreatment steps. Meanwhile, high performance liquid chromatography (HPLC) is becoming a powerful tool for the accurate and easy determination of toluene metabolites considering its decisive advantages for routine monitoring. Furthermore, recent development in HPLC could widen the usefulness of this method to solve the most complex analytical problems that could be encountered during the measurement.Keywords: toluene, toluene metabolites, preliminary test, gas chromatography, high performance liquid chromatography Abstrak Penentuan toluena dan metabolitnya dalam sampel biologi seperti urin dan darah membolehkan anggaran tahap pendedahan terhadap bahan kimia ini. Kaedah kromatografi dan kaedah awalan merupakan kaedah yang sering digunakan untuk tujuan ini pada masa kini. Kaedah awalan menggunakan ujian warna telah dibangunkan untuk penentuan kualitatif toluena dan metabolit. Kit ujian mudah alih menggunakan ujian warna meluas digunakan bagi pemeriksaan sampel urin tetapi kaedah kromatografi masih diperlukan untuk pengesahan analisis kuantitatif. Kaedah kromatografi gas adalah kaedah yang kerap digunakan bagi menentukan metabolit toluena dalam urin namun kaedah ini sering memerlukan beberapa langkah rawatan awal. Sementara itu, kromatografi cecair berprestasi tinggi (HPLC) menjadi alat yang berkuasa untuk penentuan tepat dan mudah metabolit toluena memandangkan kelebihannya yang jelas untuk pemantauan rutin. Tambahan pula, perkembangan terkini dalam HPLC boleh memperluaskan kegunaan kaedah ini bagi menyelesaikan masalah analisis rumit yang boleh dihadapi semasa pengukuran.Kata kunci: toluena, metabolit toluena, ujian awalan, kromatografi gas, kromatografi cecair berprestasi tinggi

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Section: Toluene Exposure Effects and Analysismentioning

confidence: 99%

Section: Analysis Of Vocs By Gas Chromatographymentioning

confidence: 99%

Chromatographic Determination of Toluene and Its Metabolites in Urine for Toluene Exposure – A Review

Zinalibdin¹,

Yacob²,

Sanagi

et al. 2016

MJAS

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“…Therefore, gas chromatography coupled with mass detector (GC/MS) may be considered the method of choice to identify and separate solvents of abuse, mostly when associated with the headspace (HS) sampling technique. Although GC/MS is considered the “gold standard” technique for unequivocal confirmation of results and is present in forensic laboratories, most studies found in literature use gas chromatography coupled with flame ionization detector (GC/FID) [ 18 – 22 ].…”

Section: Introductionmentioning

confidence: 99%

Multianalytical Method Validation for Qualitative and Quantitative Analysis of Solvents of Abuse in Oral Fluid by HS-GC/MS

Coppe

Borille

Fiorentin

et al. 2016

International Journal of Analytical Chemistry

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The use of oral fluid as a biological matrix to monitor the use of drugs of abuse is a global trend because it presents several advantages and good correlation to the blood level. Thus, the present work aimed to develop and validate an analytical method for quantification and detection of solvents used as inhalants of abuse in oral fluid (OF), using Quantisal™ as collector device by headspace and gas chromatography coupled with a mass detector (HS-GC/MS). Chromatographic separation was performed with a ZB-BAC1 column and the total time of analysis was 11.8 min. The method showed good linearity (correlation coefficient higher than 0.99 for all solvents). The limits of detection ranged from 0.05 to 5 mg/L, while the lower limits of quantification ranged from 2.5 to 12.5 mg/L. Accuracy, precision, matrix effect, and residual effect presented satisfactory results, meeting the criteria accepted for the validation of bioanalytical methods. The method showed good selectivity considering that, for solvents coeluting at the same retention time, resolution was performed by the mass detector. The method developed proved to be adequate when applied in OF samples from users of drugs and may be used to monitor the abuse of inhalants in routine forensic analyses.

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“…Métodos para a identificação e correta quantificação de álcoois por cromatografia gasosa com detector de ionização em chama (GC-FID) já estão bem estabelecidos na literatura e devido à sua sensibilidade, precisão e relativa especificidade, esta metodologia foi selecionada para a realização das análises de álcoois descritas neste trabalho [9][10][11][12] . A implementação da técnica analítica e a sua utilização na rotina das análises de emergência exige um processo de validação para garantir a confiabilidade do método.…”

Section: Introductionunclassified

Determinação De Etanol E Metanol Em Sangue Por Headspace / Gc-Fid Nos Casos Atendidos Pelo Centro De Controle De Intoxicações De São Paulo

Eller¹,

Zucoloto²,

Oliveira³

et al. 2016

BJFS

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Para o adequado diagnóstico e tratamento no pronto-socorro, muitas vezes é necessário saber se a condição de um paciente pode ser elucidada pela intoxicação com algum agente exógeno. Assim, a análise toxicológica é normalmente conduzida quando o paciente apresenta-se sintomático, com histórico de ingestão de determinada substância ou quando a equipe médica necessitar de diagnóstico diferencial. Geralmente, o sangue representa a matriz de escolha para essas análises, pois os efeitos farmacológicos e tóxicos podem ser, na maioria das vezes, correlacionados com a concentração do analito no organismo. O objetivo deste estudo foi a determinação de etanol e metanol em amostras provenientes de pacientes atendidos pelo Centro de Controle de Intoxicações da cidade de São Paulo. Do total de amostras analisadas (n = 151), 31 apresentaram resultados positivos para etanol, sendo 25 com concentração superior a 0,5 g.L-1. Conclui-se que a técnica de preparo de amostra utilizada é relativamente barata, fácil e rápida, o que reitera a aplicabilidade do método nas análises toxicológicas de emergência.

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Qualitative and quantitative analysis of a group of volatile organic compounds in biological samples by HS-GC/FID: application in practical cases

Cited by 17 publications

References 26 publications

Chromatographic Determination of Toluene and Its Metabolites in Urine for Toluene Exposure – A Review

Chromatographic Determination of Toluene and Its Metabolites in Urine for Toluene Exposure – A Review

Multianalytical Method Validation for Qualitative and Quantitative Analysis of Solvents of Abuse in Oral Fluid by HS-GC/MS

Determinação De Etanol E Metanol Em Sangue Por Headspace / Gc-Fid Nos Casos Atendidos Pelo Centro De Controle De Intoxicações De São Paulo

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