Qualitative and quantitative analysis of 2, 5-anhydro-d-mannitol in low molecular weight heparins with high performance anion exchange chromatography hyphenated quadrupole time of flight mass spectrometry

Lin, Yi; Zhang, Qinghua; Meng, Yao; Hao, Jie; Xie, Bingying; Gan, Hao; Li, Duxin; Dong, Kai; Zhang, Zhenqing

doi:10.1016/j.chroma.2018.07.058

Cited by 6 publications

(3 citation statements)

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“…This result confirmed that periodate treatment completely destroyed the AT pentasaccharide binding sites in these samples. D-NACH showed the tightest binding to HGF and FGF2, while NACH showed the tightest binding to TGFβ1 . The binding of D-NACH and NACH to these three heparin-binding proteins was stronger than the binding of either of the anticoagulant heparins, USP heparin and enoxaparin.…”

Section: Resultsmentioning

confidence: 89%

“…D-NACH showed the tightest binding to HGF and FGF2, while NACH showed the tightest binding to TGFβ1. 32 The binding of D-NACH and NACH to these three heparin-binding proteins was stronger than the binding of either of the anticoagulant heparins, USP heparin and enoxaparin. This is consistent with the higher content of TriS in D-NACH and NACH and the known preference of HGF, FGF2, and TGFβ1 for the more highly sulfated domain in heparin.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

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Non-Anticoagulant Low Molecular Weight Heparins for Pharmaceutical Applications

Ouyang

Zhang

et al. 2019

J. Med. Chem.

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Heparin is a polypharmacological agent with anticoagulant activity. Periodate oxidation of the nonsulfated glucuronic acid residue results in non-anticoagulant heparin derivative (NACH) of reduced molecular weight. Similar treatment of a low molecular weight heparin, dalteparin, also removes its anticoagulant activity, affording a second heparin derivative (D-NACH). A full structural characterization of these two derivatives reveals their structural differences. SPR studies display their ability to bind to several important heparin-binding proteins, suggesting potential new therapeutic applications.

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Section: Resultsmentioning

confidence: 89%

Section: ■ Results and Discussionmentioning

confidence: 99%

Non-Anticoagulant Low Molecular Weight Heparins for Pharmaceutical Applications

Ouyang

Zhang

et al. 2019

J. Med. Chem.

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“…SAX-HPLC is a means of separation relying on the interaction between negatively charged HS/heparin and the positively charged solid state support of an SAX column [44]. This method affords higher resolution than HILIC and can easily separate disaccharides with different sulfate levels, especially gratifyingly in the analysis of disaccharides obtained by the cleavage of heparins and LMWH with lyase [45].…”

Section: Sec and Sax-ms/msmentioning

confidence: 99%