Qualitative and Quantitative Analysis of Swertia Herbs by High Performance Liquid Chromatography-Diode Array Detector-Mass Spectrometry (HPLC-DAD-MS)

Wang, Zhigang; Ma, Cui; Tang, Shuhan; Xiao, Huai; Kakiuchi, Nobuko; Kida, Hiroaki; Hattori, Masao

doi:10.1248/cpb.56.485

Cited by 28 publications

(18 citation statements)

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“…Materials used in this study are summarized in Table 1; they were the same materials used in a previous study [4]. Cultivated Swertia japonica specimens were collected in Nagano Prefecture, Japan.…”

Section: Plant and Crude Drug Materialsmentioning

confidence: 99%

“…These biological effects are attributable to the major and characteristic groups of the chemical constituents of Swertia plants (xanthones, iridoids and flavonoids). Recently, chemical constituent profiling of the crude drugs derived from four Yunnanese Swertia plants and Japanese S. japonica as well as S. pseudochinensis Hara from Hebei Province was conducted using LC/MS/MS [4]. The results showed the following similarities and differences in chemical profile of the major constituents of the 6 species: (1) S. japonica and S. pseudochinensis showed high swertiamarin content and universal occurrence of irridoids and flavonoids, (2) S. decora Franch and S. delevayi Franch showed high swertiamarin but very low xanthones and flavonoids content, (3) S. binchuanensis T. N. Ho & S. W. Liu and S. punicea Hensl had relatively high flavonoids content.…”

Section: Introductionmentioning

confidence: 99%

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Phylogenetic examination of crude drugs derived from Yunnanese Swertia plants

et al. 2013

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Aiming to examine whether the genetic background of the crude drugs derived from four Yunnanese Swertia plants and their chemical constituent profiles correlate, we analyzed the nucleotide sequences of their nuclear ribosomal DNA regions including ITS1, 5.8S ribosomal RNA gene, and ITS2, together with those of Japanese S. japonica and S. pseudochinensis from Hebei Province. The result that two of the Yunnanese Swertia plants, S. binchuanensis and S. punicea, were genetically similar may explain their similarity in chemical constituent profiles. On the other hand, in spite of differences in chemical profile, S. decora and S. pseudochinensis were genetically close. The other Yunnanese Swertia plants, S. delavayi, and S. japonica, stood at intermediate positions between these two genetically similar pairs. The result suggests that although genetic background would have an influence, environmental factors, e.g., soil and weather conditions, might be critical for their production of secondary metabolites.

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Section: Plant and Crude Drug Materialsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Phylogenetic examination of crude drugs derived from Yunnanese Swertia plants

et al. 2013

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“…18,19,20 In the Cecropia genus, quantitative analysis of chlorogenic acid has been reported only for C. obtusifolia. The contents of this compound in the leaves of this species are in the range of 3.0-13.2 mg g -1 of dry weight.…”

Section: Quantification and Validation Proceduresmentioning

confidence: 99%

An HPLC-DAD method to quantification of main phenolic compounds from leaves of Cecropia Species

Costa¹,

Ortmann²,

Schenkel³

et al. 2011

J. Braz. Chem. Soc.

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Um método eficiente e reprodutível por CLAE-DAD foi desenvolvido e validado para quantificação simultânea dos compostos fenólicos majoritários (ácido clorogênico, isoorientina, orientina e isovitexina) presentes nas folhas de duas espécies de Cecropia, C. glaziovii e C. pachystachya. Das folhas de C. glaziovii e C. pachystachya foram isolados os flavonoides C-glicosídeos isoorientina e isovitexina e identificados em ambas as espécies o ácido clorogênico (ácido 3-O-cafeoilquínico) e o flavonoide O-glicosídeo isoquercitrina. O flavonoide C-glicosídeo orientina foi isolado apenas da espécie C. pachystachya. O ácido clorogênico mostrou-se como composto majoritário em ambas as espécies analisadas (11,1 mg g -1 de extrato de C. glaziovii e 27,2 mg g -1 de extrato de C. pachystachya), e em relação aos flavonoides quantificados, isovitexina se apresentou como o flavonoide C-glicosídeo majoritário para C. glaziovii (4,6 mg g -1 extrato) e isoorientina majoritário para C. pachystachya (17,3 mg g -1 de extrato).An efficient and reproducible HPLC-DAD method was developed and validated for the simultaneous quantification of major compounds (chlorogenic acid, isoorientin, orientin and isovitexin) present in the leaves of two Cecropia species, C. glaziovii and C. pachystachya. From the leaves of C. glaziovii and C. pachystachya were isolated the C-glycosylflavones isoorientin and isovitexin and identified on both species chlorogenic acid (3-O-caffeoylquinic acid) and the O-glycosylflavonol isoquercitrin. The C-glycosylflavone orientin was isolated only from C. pachystachya. Chlorogenic acid was the major compound in both species (11.1 mg g -1 of extract of C. glaziovii and 27.2 mg g -1 of extract of C. pachystachya) and for the flavonoids quantified, isovitexin was the main C-glycosylflavonoid for C. glaziovii (4.6 mg g -1 of extract) and isoorientin the main one for C. pachystachya (17.3 mg g -1 of extract).

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“…Gentiana siphonantha Maxim., a perennial herb in the Gentianaceae family, has been a traditional Chinese medicine for more than 2000 years for the treatment of rheumatoid arthritis, jaundice hepatitis, constipation, pain, and hypertension [25,26,27]. Previous studies on G. siphonantha have shown that this medical herb is rich in iridoids, especially gentiopicroside [28,29], well-known phytochemicals in a number of folk medicinal plants with anti-inflammatory, antibacterial, free radical-scavenging, and hepatoprotective activities [30,31,32,33]. The above plants have anti-inflammatory, analgesic or swelling-decreasing activities.…”

Section: Introductionmentioning

confidence: 99%

Antidermatophyte Activity of the Gentiopicroside-Rich n-Butanol Fraction from <b><i>Gentiana siphonantha</i></b> Maxim. Root on a Guinea Pig Model of Dermatophytosis

et al. 2018

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Background:Gentiana siphonantha Maxim. is a traditional medicine for the treatment of rheumarthritis, icterepatitis, pain and hypertension; it is rich in gentiopicroside with anti-inflammatory, antibacterial, and free radical-scavenging activities. This study was to evaluate the antidermatophyte activity of G. siphonantha on a guinea pig model in vitro and in vivo. Material andMethods: The antidermatophyte activities of 10 plants were tested by the broth microdilution method. Fractions and an extract of G. siphonantha were tested against Trichophyton mentagrophytes by the disc diffusion method. The morphological changes of T. mentagrophytes were observed. Component analysis of the n-butanol (n-BuOH) fraction was made by HPLC. Finally, the antifungal activity in an in vivo guinea pig model of dermatophytosis was examined. Results:G. siphonantha had strong antidermatophyte activity with MIC₅₀ values of 32-64 μg/mL. The n-BuOH fraction of G. siphonantha showed the most potent activity compared to the other fractions. After being exposed to the n-BuOH fraction at 80 and 160 μg/mL, the hyphae were distorted and collapsed. Gentiopicroside is the main active ingredient in the n-BuOH fraction of G. siphonantha. The lesion scores of the guinea pig model of dermatophytosis significantly declined in the 10% and 30% extract and positive control groups in comparison with the untreated control group. Periodic acid-Schiff and hematoxylin/eosin staining displayed similar results. Conclusion: The n-BuOH fraction of G. siphonantha demonstrated antidermatophyte efficacy in experimental dermatophytosis.

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Qualitative and Quantitative Analysis of Swertia Herbs by High Performance Liquid Chromatography-Diode Array Detector-Mass Spectrometry (HPLC-DAD-MS)

Cited by 28 publications

References 28 publications

Phylogenetic examination of crude drugs derived from Yunnanese Swertia plants

Phylogenetic examination of crude drugs derived from Yunnanese Swertia plants

An HPLC-DAD method to quantification of main phenolic compounds from leaves of Cecropia Species

Antidermatophyte Activity of the Gentiopicroside-Rich n-Butanol Fraction from <b><i>Gentiana siphonantha</i></b> Maxim. Root on a Guinea Pig Model of Dermatophytosis

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