Qualitative and Quantitative Analysis of Phenolic Acids, Flavonoids and Iridoid Glycosides in Yinhua Kanggan Tablet by UPLC-QqQ-MS/MS

Abstract

A simple, rapid and specific ultra-performance liquid chromatography-triple quadrupole mass spectrometry method was developed for the analysis of 29 bioactive components (10 phenolic acids, 16 flavonoids, and three iridoid glycosides) in Yinhua Kanggan tablet (YHKGT), a herbal prescription used for treating upper respiratory infections, fevers, coughs and pharyngalgia. The separation was successfully achieved using a Waters Cortecs UPLC C18 column (50 × 2.1 mm, 1.6 μm) and gradient elution with water-0.1% formic acid and acetonitrile. Polarity switching mode was used in the optimization of multiple reaction monitoring conditions. The analytical method was validated for linearity, precision and accuracy. Calibration curves for the 29 marker compounds showed good linear regression (r > 0.9982). The limits of detection (LOD) and limits of quantification (LOQ) for the 29 analytes were in the range of 0.03-4.99 ng/mL and 0.16-14.87 ng/mL, respectively. The relative standard deviation (RSD) values of intra-day precision, inter-day precision, repeatability, and stability were less than 2.79%, 4.87%, 4.18% and 4.71%, respectively. The recoveries of the 29 marker compounds were in the range of 94.67%-104.78% (RSD ≤ 4.72%). These results have shown that this developed method was efficient for the quality evaluation of YHKGT.

Bioactivities of Anethole, Astragalin and Cryptochlorogenic Acid Extracted from Anise Oil and Moringa oleifera on the Keratinase Gene Expression of Trichophyton rubrum

“…Lin et al 30 reported that cryptochlorogenic acid and astragalin were two of the basic compounds present in M. oleifera leaf extracts with molecular weights of 355 and 447, respectively, which is consistent with the results of the present study. Cryptochlorogenic acid possesses inhibitory effects against proteases and many other enzymes, including hepatic glucose-6-phosphatase 31 .…”

Iridoid Glycosides Fraction Isolated from Veronica ciliata Fisch. Protects against Acetaminophen‐Induced Liver Injury in Mice

“…The PDA detection was conducted at 260 nm. Levels of the IGF were quantitated using Empower software version 3.0 (Waters) [ 10 , 16 ].…”

Contribution of Flavonoids and Iridoids to the Hypoglycaemic, Antioxidant, and Nitric Oxide (NO) Inhibitory Activities of Arbutus unedo L.

“…A. unedo extracts were solubilized in methanol, filtered, and analysed using an HPLC (U-3000, Thermo, Courtaboeuf, France) coupled to an ESI-QTOF mass spectrometer (Maxis II, Bruker, Champs sur Marne, France), as previously described [22] with some modifications as shown below. The chromatographic separation was performed on a C18 column (Acclaim RSLC polar advantage II, 100 × 2.1 mm, 2.2 µm) maintained at a temperature of 35 • C, with a speed of flow of 0.3 mL/min.…”