Qualitative and quantitative analysis of phenolic compounds in blueberries and protective effects on hydrogen peroxide‐induced cell injury

Abstract

This work was conducted to optimize an accelerated solvent extraction for ultra high performance liquid chromatography-mass spectrometry/mass spectrometry analysis of blueberry phenolic compounds. The conditions for accelerated solvent extraction were verified using response surface methodology to obtain the following optimized conditions: ethanol concentration (pH = 3), 48%; temperature, 50°C, and static cycle times, 3. Further, ultra high performance liquid chromatography with quadrupole Exactive Orbitrap mass spectrometry and ultra high performance liquid chromatography with triple-quadrupole tandem mass methods for determination of the detailed phenolic composition were developed and validated. Total of 81 phenolic compounds were identified by ultra high performance liquid chromatography with quadrupole Exactive Orbitrap mass spectrometry including 23 anthocyanins, 32 flavonols, 11 proanthocyanidins, 2 other flavonoids, and 13 phenolic acids. Fifty-one of these compounds have been simultaneously quantified by ultra high performance liquid chromatography with triple-quadrupole tandem mass including 31 anthocyanins, 8 flavonols, 6 proanthocyanidins, 2 other flavonoids, and 8 phenolic acids. Malvidin-dinhexoside has, for the first time, been detected in wild. Moreover, by verifying the protection on PC12 cells against oxidative damage, it was showed that the phenolic extracts (500 µg/mL) can improve significantly the viability (9.26-24.78%) of hydrogen peroxide-induced PC12 cells, activities of superoxide dismutase (34.59-37.90 U/mg) and glutathione peroxidase (6.87-14.42 mU/mg) and decrease the

Characterization and quantification of chemical constituents in Fuzhuan brick tea using ultra‐high‐performance liquid chromatography–mass spectrometry

“…Peak 4 with an [M − H] − ion at m/z 343.06 was characterized as galloylquinic acid [16,21], while peak 12, with m/z 353.08, was characterized as chlorogenic acid [19], according to the reference. Peak 8 showed an MS ion at m/z 179.03, implying a possible substitution of caffeic acid, which was identified as neochlorogenic acid [19,22,23]. The characteristic ion of peak 9 at m/z 163.04 implied the possible substitution of p-coumaric acid, as reported previously [15], and was confirmed to be coumaroylquinic acid.…”

Novel l-Cysteine Incomplete Degradation Method for Preparation of Procyanidin B2-3′-O-Gallate and Exploration of its in Vitro Anti-inflammatory Activity and in Vivo Tissue Distribution

“…The MS conditions were as described by Wang et al with some modifications. 22 Briefly, ion mode, negative ESI; scanning mode, precursor ion scan; scan range, 100−1500 Da; capillary voltage, 3.0 kV; collision energy, 10−30 eV; sheath gas pressure, 30 bar; auxiliary gas pressure, 10 bar; ion transfer tube temperature, 320 °C; nebulizer temperature, 350 °C.…”

High‐performance liquid chromatography quantitative analysis of ephedrine alkaloids in Ephedrae Herba on a perfluorooctyl stationary phase

“…Several influencing factors were also investigated to optimize chromatographic conditions, including buffer concentration and pH of mobile phase. Under the optimized conditions, method validation was carried out to verify the method stability [25][26][27][28][29]. This research brings new perspectives for the quantitative analysis of alkaloids in Ephedrae Herba.…”