Qualitative and quantitative assesment of initial bacterial plaque formation on different prosthetic restorative materials

Abstract

Aim: The aim of this study was to determine the structure of bacterial plaque qualitatively and quantitatively and to evaluate the effect of restorative material variation on pellicle composition and bacterial adhesion. Materials and Method: Titanium, Au-Ag-Pd alloy, Ni-Cr alloy, an all-ceramic system and a feldspathic porcelain system were used to evaluate bacterial adhesion and plaque composition characteristics. Acquired pellicle was analyzed by using sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). Single colony isolated groups on samples were identified by using BBL-Crystal System. Statistical analysis was performed by using Kruskal Wallis Analysis of Variance (ANOVA). Results: Acquired pellicle analysis by SDS-PAGE revealed amylase content for each group except feldspathic porcelain.Significant qualitative and quantitative differences were found among the materials for bacterial plaque content. Microorganism types varied most for feldspathic porcelain surface. The least variety of microorganisms were found on Cr-Ni. The lowest adhesion was observed on feldspathic porcelain. Conclusion: Initial bacterial plaque shows material specific differences for compositional structure. Bacterial adhesion for each material shows internal compositional variations. Streptococci were found to be most revealed species for all materials studied.