Qualitative and quantitative changes in acetylcholine receptor distribution at the neuromuscular junction following free muscle transfer

Jiang, Hua; Samuel, Tay Sam Wah; Kumar, Veerendra

doi:10.1002/mus.10046

Cited by 4 publications

(3 citation statements)

References 29 publications

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“…It has been previously proposed that the morphological and functional state of AChRs in muscle fibers is closely correlated with the affiliated innervation 25. This hypothesis had been supported by the study of spinal nerve crush injury model in rats, which showed that AChRs at end plates had crispation, depressed density, disordered structure, and disaggregation through the α‐bungarotoxin fluorescent binding agent staining 26. The degree of the nerve damage was in accordance with the extent of AChR impairment and with the level of muscle weakness.…”

Section: Discussionmentioning

confidence: 83%

Differences in sensitivity to rocuronium among orbicularis oris muscles innervated by normal or damaged facial nerves and gastrocnemius muscle innervated by somatic nerve in rats

Zhou

Chi

et al. 2012

The Laryngoscope

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The affinity of AChR at end plates and different number of AChR per unit fiber cross-sectional area may be the mechanisms for differential sensitivities to neuromuscular blockers between facial nerve-innervated muscles and somatic nerve-innervated muscles. The lower EEMG responses in the impaired facial nerve-innervated muscles may result from the lower AChR density at end plates compared with the normal facial nerve-innervated muscles.

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Section: Discussionmentioning

confidence: 83%

Differences in sensitivity to rocuronium among orbicularis oris muscles innervated by normal or damaged facial nerves and gastrocnemius muscle innervated by somatic nerve in rats

Zhou

Chi

et al. 2012

The Laryngoscope

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“…21 Although the number of AChRs at the reinnervated neuromuscular junction of transferred muscle flap was studied, 11 this study focused on the correlation between contractile function recovery and changes of AChR and expression of its subunits of transferred muscle flap.…”

Section: Discussionmentioning

confidence: 99%

“…The number of acetylcholine receptors at the reinnervated neuromuscular junction was found still remained lower 30 weeks after transfer. 10,11 The AChR is an integral membrane channel protein, that is, a key element in cholinergic synaptic transmission. Experimental studies have demonstrated that mammalian muscle mainly expressed two subunits of AChR channels, a low conductance fetal subunit AChRg and a high conductance adult subunit AChRe.…”

mentioning

confidence: 99%

Correlation of contractile function recovery with acetylcholine receptor changes in a rat muscle flap model

et al. 2010

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The aim of this study was to investigate the correlation between contractile function recovery and changes of acetylcholine receptors (AChR) in a transferred muscle flap following reinnervation. Orthotopic transfer of the gracilis muscle flap with repair of its nerve was performed bilaterally in 48 rats. The rats were randomly divided into six experimental groups based on the time intervals for assessments (1, 4, 5, 10, 20, and 30 weeks). Sixteen gracilis muscle samples from eight rats without surgery were used as the controls. In each group, muscle contractile force and weight were measured (n = 16). The AChR numbers (n = 8) and subunits (epsilon and gamma) mRNA (n = 8) were examined using [(125)I]-alpha-bungarotoxin and fluorescent quantitative-PCR. The results showed the AChR numbers in the muscle flap increased from 4 to 20 weeks after reinnervation and correlated with recovery of the tetanic contraction force. However, correlation between the increase of AChR number with the specific tension (peak contractile force normalized to wet muscle weight) was only found from 4 to 10 weeks postoperatively. The expression of gamma-subunit mRNA increased at the early period after flap transfer and then decreased rapidly, whereas the epsilon-subunit mRNA recovered gradually since fourth week postoperatively. A small amount of gamma-subunit mRNA could still be detected at 30 weeks after surgery. In conclusion, following reinnervation of the transferred muscle flap, the contractile functional recovery is partially correlated to increase of the AChRepsilon. Our findings may provide evidence for further study of improving muscle function in functional reconstruction by targeting the AChR.

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Free Flap Functional Muscle Transfers

Garcia

Ruch

2016

Hand Clinics

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Qualitative and quantitative changes in acetylcholine receptor distribution at the neuromuscular junction following free muscle transfer

Cited by 4 publications

References 29 publications

Differences in sensitivity to rocuronium among orbicularis oris muscles innervated by normal or damaged facial nerves and gastrocnemius muscle innervated by somatic nerve in rats

Differences in sensitivity to rocuronium among orbicularis oris muscles innervated by normal or damaged facial nerves and gastrocnemius muscle innervated by somatic nerve in rats

Correlation of contractile function recovery with acetylcholine receptor changes in a rat muscle flap model

Free Flap Functional Muscle Transfers

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