Qualitative and quantitative determinations of pyridalyl and metabolites in excrement of two representative Lepidoptera pests

Wang, Lingling; Nie, Yuting; Wang, Yujiao; Wang, Zhenyu; Xiong, Bo

doi:10.1039/c5ra21984a

Cited by 1 publication

(1 citation statement)

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“…Moreover, their retention times were similar to those of their counterparts in a reported work with an SPE column. 47 These results demonstrated the applicability of the pre-treatment module as an alternative of SPE to extract pyridalyl and its metabolites in the excrements of H. armigera . MP2 was only observed in the acetone fraction, while pyridalyl and MP3 were detected in both methanol and acetone elutions.…”

Section: Resultsmentioning

confidence: 68%

On-line pre-treatment, separation, and nanoelectrospray mass spectrometric determinations for pesticide metabolites and peptides based on a modular microfluidic platform

et al. 2018

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In order to address time-consuming sample pre-treatment and separation prior to mass spectrometry (MS) identifications, highly integrated chips were developed, but damage to any functional unit in these chips would result in complete replacement. Herein, we propose a modular microfluidic platform comprising pre-treatment, liquid chromatography (LC) separation and nanoelectrospray ionization (nESI) chips for on-line enrichment, separation and nESI MS detection of pesticide metabolites and peptides. The pretreatment chip is applicable in enriching pyridalyl and its metabolites, and it achieves optimal desalination efficiency, 98.5%, for polymerase chain reaction products. Additionally, the LC separation chip was fully characterised, and it demonstrated satisfactory separation efficiency, quantification ability and pressure durability. Finally, the modular microfluidic platform was used to identify the peptides in trypsin-digested casein. Four additional peptides were identified, indicating an improvement in detection ability compared with using off-line zip tips coupled with MS investigations. Because the proposed modular platform can significantly reduce manual work, it would be a potential tool to achieve high throughput and automatic MS identifications with low sample consumptions. † Electronic supplementary information (ESI) available: One PDF le provides supporting information for pre-treatment and separation modules congurations, instrumental setup of the modular platform, PCR experimental details, and identication of reserpine with the proposed platform. See

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Section: Resultsmentioning

confidence: 68%

On-line pre-treatment, separation, and nanoelectrospray mass spectrometric determinations for pesticide metabolites and peptides based on a modular microfluidic platform

et al. 2018

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Qualitative and quantitative determinations of pyridalyl and metabolites in excrement of two representative Lepidoptera pests

Abstract: Qualitative and quantitative SPE followed by HPLC-TOF/MS determination of pyridalyl and its potential metabolites in the excrement of Helicoverpa armigera (H. armigera) and Spodoptera exigua (S. exigua) was developed.

Cited by 1 publication

References 26 publications

On-line pre-treatment, separation, and nanoelectrospray mass spectrometric determinations for pesticide metabolites and peptides based on a modular microfluidic platform

On-line pre-treatment, separation, and nanoelectrospray mass spectrometric determinations for pesticide metabolites and peptides based on a modular microfluidic platform

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