Qualitative and quantitative evaluation of coprological and serological techniques for the diagnosis of fasciolosis in cattle

Charlier, Johannes; Meulemeester, L. De; Claerebout, Edwin; Williams, Diana; Vercruysse, Jozef

doi:10.1016/j.vetpar.2008.01.035

Cited by 122 publications

(120 citation statements)

References 19 publications

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“…Similar observations were reported from Switzerland, where infected livers were detected only in two-thirds of seropositive animals at abattoir inspection (Rapsch et al, 2006). Charlier et al (2008) showed that only one third of F. hepatica infected livers was detected by standard meat inspection cuts, that Fig. 2.…”

Section: Discussionsupporting

confidence: 84%

Distribution of Fasciola hepatica in Swedish dairy cattle and associations with pasture management factors

2015

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Abstract. The geographic distribution of Fasciola hepatica infection in relation to management routines was studied in Swedish dairy herds by testing for F. hepatica antibodies with the enzyme-linked immunosorbent assay (ELISA). In addition, all farmers were sent a questionnaire asking for information about type of production, management routines and historical record of F. hepatica at slaughter. A total of 176 farmers (41%) responded to the questionnaire. A total of 426 bulk tank milk (BTM) samples were randomly selected from the period September to October 2012 representing approximately 10% of all herds in Sweden. The overall seroprevalence was 25% (n = 107; 95% confidence interval = 21-29%) with a concentration of herds located in south-western Sweden. Among the seropositive herds, 31 (29%) had antibody levels indicating production loss. There were no significant differences in seropositivity between organic and conventional herds or due to pasture management routines. The length of grazing period, which increased the risk for heifers, was found to be the most influential factor. A discrepancy was noted between reported F. hepatica presence at meat inspection and herds that were seropositive based on BTM-ELISA results. Although the largest proportion of seropositive BTM samples (80%) came from herds where liver fluke presence had been observed at meat inspection after slaughter, seropositive BTM samples were also diagnosed in five herds (17%) with no remarks at slaughter. In conclusion, F. hepatica is a common parasite in Swedish dairy herds and the month of heifer turn-out and the grazing period length were the most influential factors observed.

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Section: Discussionsupporting

confidence: 84%

Distribution of Fasciola hepatica in Swedish dairy cattle and associations with pasture management factors

2015

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“…A number of diagnostic tools have been developed for single or simultaneous detection/discrimination of F. hepatica and F. gigantica, including morphological (using shape and size and morphometric features), immunodiagnostic (using monoclonal antibodies or copro-antigen (extracted from eggs in feces) and metacercarial/Fasciola-specific antigens for ELISA) and DNA-related loop-mediated isothermal amplification (LAMP), PCR (single or multiplex), restriction enzymatic, and sequencing methods (2,3,5,11,12,13,35). All the diagnostic methods developed so far have contributed to fast, accurate, and specific detection of Fasciola spp.…”

Section: Discussionmentioning

confidence: 99%

“…To date, various diagnostic techniques for the identification and discrimination of Fasciola spp. have been developed, including the traditional detection of eggs in feces directly or after Kato-Katz based sedimentation (19,43), the morphological examination of adults (38), the lateral flow immunoassay for serodiagnosis (28), and the enzyme-linked immunosorbent assay (indirect ELISA) (12,13,32,36).…”

mentioning

confidence: 99%

Development and Evaluation of a Single-Step Duplex PCR for Simultaneous Detection of Fasciola hepatica and Fasciola gigantica (Family Fasciolidae, Class Trematoda, Phylum Platyhelminthes)

Nguyen

et al. 2012

J Clin Microbiol

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A single-step multiplex PCR (here referred to as a duplex PCR) has been developed for simultaneous detection and diagnosis of Fasciola hepatica and F. gigantica. These species overlap in distribution in many countries of North and East Africa and Central and Southeast Asia and are similar in egg morphology, making identification from fecal samples difficult. Based on a comparative alignment of mitochondrial DNA (mtDNA) spanning the region of cox1-trnT-rrnL, two species-specific forward primers were designed, FHF (for F. hepatica) and FGF (for F. gigantica), and a single reverse primer, FHGR (common for both species). Conventional PCR followed by sequencing was applied using species-specific primer pairs to verify the specificity of primers and the identity of Fasciola DNA templates. Duplex PCR (using three primers) was used for testing with the DNA extracted from adult worms, miracidia, and eggs, producing amplicons of 1,031 bp for F. hepatica and 615 bp for F. gigantica. The duplex PCR failed to amplify from DNA of other common liver and intestinal trematodes, including two opisthorchiids, three heterophyids, an echinostomid, another fasciolid, and a taeniid cestode. The sensitivity assay showed that the duplex PCR limit of detection for each Fasciola species was between 0.012 ng and 0.006 ng DNA. Evaluation using DNA templates from 32 Fasciola samples (28 adults and 4 eggs) and from 25 field-collected stools of ruminants and humans revealed specific bands of the correct size and the presence of Fasciola species. This novel mtDNA duplex PCR is a sensitive and fast tool for accurate identification of Fasciola species in areas of distributional and zonal overlap.

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“…ELISA was selected in the current study for evaluation of the diagnostic potency of the isolated glycoproteins based on its high sensitivity and possibility of processing many serum samples simultaneously (Cornelissen et al 2001;Charlier et al 2008). In the present study, it was concluded that FII glycoprotein could detect experimental F. gigantica infection in rabbits as early as one week post infection with high significance than FI and crude extract.…”

Section: Serine (Ser)mentioning

confidence: 69%

“…The early diagnosis of fasciolosis during prepatent period using antibody detection tests is an essential method for arresting its negative impact on productivity and preventing economic losses (Sanchez-Andrade et al 2000;Dixit et al 2004;Kumar et al 2008). ELISA-based antibody detection systems using different protein antigens are excellent for the early diagnosis of fasciolosis Espinoza et al 2007;Mezo et al 2007;Charlier et al 2008;Kumar et al 2008).…”

Section: Introductionmentioning

confidence: 99%

The role of Ser-(Arg-Ser-Arg-Ser-GlucNAc)19-GlucNAc Fasciola gigantica glycoprotein in the diagnosis of prepatent fasciolosis in rabbits

Abdel-Rahman¹,

Mohamed

Abdel‐Rahman

et al. 2014

J Parasit Dis

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In the present study, the carbohydrate structures associated with Fasciola gigantica adult worm were identified by indirect hemagglutination inhibition test. Glucose was found to be the main monosaccharide associated with the fluke. According to indirect hemagglutination inhibition results, purification of glycoprotein fractions from worm crude extract was carried out by affinity chromatography immobilized glucose agarose gel and Con-A lectin columns. The isolated glycoprotein fractions, FI and FII, were characterized by SDS-PAGE which revealed one band in FI of 26 kDa and another one band of 19.5 kDa in FII compared with 12 bands associated with whole worm extract. Both fractions were also characterized by isoelectric focusing technique which proved that both bands were acidic in nature with pIs 6.4 and 6.5 respectively. The comparative diagnostic evaluation of the two isolated glycoprotein fractions and crude extract of experimental fasciolosis in rabbits by ELISA revealed that FII was more potent in the diagnosis during prepatent (first week post infection) and patent periods (10 weeks post infection) than FI and crude extract. Moreover, infected rabbit sera at ten weeks post infection identified both bands; 26 and 19.5 kDa in western blot analysis confirming its immunodiagnostic activities which was proved previously by ELISA. FII proved potency in diagnosis of fasciolosis in 200 buffalo serum samples of different ages and sexes using ELISA which recorded 95 % positive and 5 % negative samples. Moreover, the detailed structural analyses of the most potent fraction, F11, using mass spectrum was made and elucidated chemical structure; O-glycan [Ser-(Arg-Ser-Arg-Ser-GlucNAc) 19 -GlucNAc]. The present result introduces GlucNAc rich fraction of F .gigantica that can be used successfully in the diagnosis of acute and chronic fasciolosis.

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Qualitative and quantitative evaluation of coprological and serological techniques for the diagnosis of fasciolosis in cattle

Cited by 122 publications

References 19 publications

Distribution of Fasciola hepatica in Swedish dairy cattle and associations with pasture management factors

Distribution of Fasciola hepatica in Swedish dairy cattle and associations with pasture management factors

Development and Evaluation of a Single-Step Duplex PCR for Simultaneous Detection of Fasciola hepatica and Fasciola gigantica (Family Fasciolidae, Class Trematoda, Phylum Platyhelminthes)

The role of Ser-(Arg-Ser-Arg-Ser-GlucNAc)19-GlucNAc Fasciola gigantica glycoprotein in the diagnosis of prepatent fasciolosis in rabbits

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