Qualitative and Semiquantitative Analysis of Fecal Bifidobacterium Species in Centenarians Living in Bama, Guangxi, China

Fang, Wang; Huang, Guohong; Cai, Da; Li, Danlei; Liang, Xiaolin; Yu, Ting; Shen, Peihong; Su, Haiyan; Liu, Jidong; Gu, Hongcang; Zhao, Ming; Li, Quanyang

doi:10.1007/s00284-015-0804-z

Cited by 33 publications

(18 citation statements)

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“…Previous study reported the detection of B. gallinarum/B. pullorum / B. saecularmay, B. minimum and B. mongoliense in Chinese centenarian [39]. However, these species were not detected in the five Japanese centenarians in the present study.…”

Section: Discussioncontrasting

confidence: 80%

“…These adult-type predominant Bifidobacterium species persisted in elderly, with an increase in the prevalence of B. dentium species [8, 21, 28]. Recently, Wang et al [39] revealed that the faecal Bifidobacterium species in centenarians of Chinese descent were different from those of young elderlies. Despite the common faecal Bifidobacterium species, including B. dentium , B. longum ssp.…”

Section: Introductionmentioning

confidence: 99%

“…longum , B. thermophilum , members of the B. catenulatum group, and B. adolescentis in younger elderlies and centenarians, centenarians were reported to possess certain unique species, such as B. minimum , B. gallinarum/B. pullorum / B. saecularmay and B. mongoliense , which were absent in the younger elderlies [39]. …”

Section: Introductionmentioning

confidence: 99%

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Age-Related Changes in the Composition of Gut Bifidobacterium Species

et al. 2017

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Bifidobacteria are one of the major components in human microbiota that are suggested to function in maintaining human health. The colonization and cell number of Bifidobacterium species in human intestine vary with ageing. However, sequential changes of Bifidobacterium species ranging from newborns to centenarians remain unresolved. Here, we investigated the gut compositional changes of Bifidobacterium species over a wide range of ages. Faecal samples of 441 healthy Japanese subjects between the ages of 0 and 104 years were analysed using real-time PCR with species-specific primers. B. longum group was widely detected from newborns to centenarians, with the highest detection rate. B. breve was detected in approximately 70% of children under 3 years old. B. adolescentis and B. catenulatum groups were predominant after weaning. B. bifidum was detected at almost all ages. The detection rate of B. dentium was higher in the elderly than in other ages. B. animalis ssp. lactis was detected in 11.4% of the subjects and their ages were restricted. B. gallinarum goup was detected in only nine subjects, while B. minimum and B. mongoliense were undetected at any age. The presence of certain Bifidobacterium groups was associated with significantly higher numbers of other Bifidobacterium species/subspecies. Inter-species correlations were found among each species, exception for B. animalis ssp. lactis. These results revealed the patterns and transition points with respect to compositional changes of Bifidobacterium species that occur with ageing, and the findings indicate that there may be symbiotic associations between some of these species in the gut microbiota.Electronic supplementary materialThe online version of this article (doi:10.1007/s00284-017-1272-4) contains supplementary material, which is available to authorized users.

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Section: Discussioncontrasting

confidence: 80%

Section: Introductionmentioning

confidence: 99%

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Age-Related Changes in the Composition of Gut Bifidobacterium Species

et al. 2017

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“…*P < 0.05. glycolytic enzyme essential for GOS metabolism that typically is genetically linked with a LacS permease responsible for transport and internalization of GOS (6). B. dentium has been less thoroughly characterized, is mostly associated with the oral cavity, and recently was shown to be increased in the stools of 100-to 108-y-old centenarians living in Bama, Guangxi (China) as compared with younger (80-to 99-y-old) elderly persons (19).…”

Section: Discussionmentioning

confidence: 99%

Impact of short-chain galactooligosaccharides on the gut microbiome of lactose-intolerant individuals

Azcárate-Peril

Ritter²,

Savaiano

et al. 2017

Proc. Natl. Acad. Sci. U.S.A.

194

131

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Directed modulation of the colonic bacteria to metabolize lactose effectively is a potentially useful approach to improve lactose digestion and tolerance. A randomized, double-blind, multisite placebo-controlled trial conducted in human subjects demonstrated that administration of a highly purified (>95%) short-chain galactooligosaccharide (GOS), designated "RP-G28," significantly improved clinical outcomes for lactose digestion and tolerance. In these individuals, stool samples were collected pretreatment (day 0), after GOS treatment (day 36), and 30 d after GOS feeding stopped and consumption of dairy products was encouraged (day 66). In this study, changes in the fecal microbiome were investigated using 16S rRNA amplicon pyrosequencing and high-throughput quantitative PCR. At day 36, bifidobacterial populations were increased in 27 of 30 of GOS subjects (90%), demonstrating a bifidogenic response in vivo. Relative abundance of lactose-fermenting Bifidobacterium, Faecalibacterium, and Lactobacillus were significantly increased in response to GOS. When dairy was introduced into the diet, lactosefermenting Roseburia species increased from day 36 to day 66. The results indicated a definitive change in the fecal microbiome of lactose-intolerant individuals, increasing the abundance of lactosemetabolizing bacteria that were responsive to dietary adaptation to GOS. This change correlated with clinical outcomes of improved lactose tolerance.A limited ability to digest lactose occurs when the intestinal lactase enzyme is reduced in the brush border of the small bowel mucosa. Consumption of dairy foods by lactose-intolerant individuals may result in clinical symptoms including abdominal pain, diarrhea, bloating, flatulence, and abdominal cramping. In these cases, lactose travels through the gastrointestinal tract and is fermented in the colon, producing acetate, carbon dioxide, hydrogen gas, and methane by gas-producing microbes. Approximately 75% of the global human population are lactose maladsorbers (1, 2). In the United States, it is estimated that up to 80 million Americans are at risk for lactose intolerance.Efforts to address lactose intolerance include avoidance of dairy foods, reduction in the lactose content of milk through treatment with microbial lactases, and the use of lactase enzymes to process milk before dairy consumption (3). Savaiano et al. conducted a randomized, double-blind, parallel-group, placebocontrolled study at two sites in the United States (4). A high purity (>95%) galactooligosaccharide (GOS/RP-G28) or a placebo (Sweetose; Tate & Lyle Ingredients) was administered in increasing doses to 62 lactose-intolerant subjects for 36 d. Subjects refrained from consuming lactose during the GOS treatment period. After GOS treatment, subjects reintroduced dairy products into their diets for an additional 30 d and then were challenged for lactose digestion and were evaluated for symptoms improvement via a Likert scale. Subjects consuming GOS trended toward improvement in overall symptoms after 36 d of ...

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“…A further decline is then seen as we enter the elderly phase of life [35], which interestingly also correlates to a decrease in immune function, so-called immunosenescence. Exactly when or why this happens is still unclear, but higher bifidobacteria levels in the elderly are correlated with health and longevity [36,37].…”

Section: Bifidobacterium Across the Life Coursementioning

confidence: 99%

Exploring the role of the microbiota member Bifidobacterium in modulating immune-linked diseases

O’Neill

Schofield

Hall

2017

Emerging Topics in Life Sciences

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The gut-associated microbiota is essential for multiple physiological processes, including immune development. Acquisition of our initial pioneer microbial communities, including the dominant early life genus Bifidobacterium, occurs at a critical period of immune maturation and programming. Bifidobacteria are resident microbiota members throughout our lifetime and have been shown to modulate specific immune cells and pathways. Notably, reductions in this genus have been associated with several diseases, including inflammatory bowel disease. In this review, we provide an overview of bifidobacteria profiles throughout life and how different strains of bifidobacteria have been implicated in immune modulation in disease states. The focus will be examining preclinical models and outcomes from clinical trials on immune-linked chronic conditions. Finally, we highlight some of the important unresolved questions in relation to Bifidobacterium-mediated immune modulation and implications for future directions, trials, and development of new therapies.

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Qualitative and Semiquantitative Analysis of Fecal Bifidobacterium Species in Centenarians Living in Bama, Guangxi, China

Cited by 33 publications

References 34 publications

Age-Related Changes in the Composition of Gut Bifidobacterium Species

Age-Related Changes in the Composition of Gut Bifidobacterium Species

Impact of short-chain galactooligosaccharides on the gut microbiome of lactose-intolerant individuals

Exploring the role of the microbiota member Bifidobacterium in modulating immune-linked diseases

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