Qualitative detection of corticosteroids in equine biological fluids and the comparison of relative dexamethasone metabolite/dexamethasone concentration in equine urine by micro-liquid chromatography—mass spectrometry

Skrabalak, Dale S.; Covey, Thomas R.; Henion, Jack D.

doi:10.1016/s0021-9673(01)90753-8

Cited by 22 publications

(3 citation statements)

References 13 publications

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“…Numerous methods such as radioimmunoassay, immunoaffinity chromatography, gas chromatography, immunoassay, HPLC, and LC‐MS have been developed for the screening and confirmation of DXM and other steroids in equine post‐race urine samples (Skrabalak et al. , 1984; Friedrich et al.…”

Section: Discussionmentioning

confidence: 99%

“…Numerous methods such as radioimmunoassay, immunoaffinity chromatography, gas chromatography, immunoassay, HPLC, and LC-MS have been developed for the screening and confirmation of DXM and other steroids in equine post-race urine samples (Skrabalak et al, 1984;Friedrich et al, 1992;Stanley et al, 1993;Gu et al, 1996;Ribeiro Neto et al, 1997a,b;Grippa et al, 2000;Tang et al, 2001). These methods were developed for screening and confirmation for forensic purposes and were not specific or sufficiently sensitive for the adequate determination of pharmacokinetics of DXM in the equine.…”

Section: Discussionmentioning

confidence: 99%

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Pharmacokinetics of dexamethasone with pharmacokinetic/pharmacodynamic model of the effect of dexamethasone on endogenous hydrocortisone and cortisone in the horse

Soma

Uboh

Luo

et al. 2005

Vet Pharm & Therapeutics

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A compartmental model was used to describe the pharmacokinetics of dexamethasone (DXM) and changes in the plasma concentration of endogenous cortisone (COR) and hydrocortisone (HYD) following intravenous (i.v.) administration of DXM (0.05 mg/kg) in horses. Quantification of DXM, COR and HYD in equine plasma was achieved using liquid chromatography interfaced with triple spray quadrupole quantum tandem mass spectrometry (LC/TSQ-MS/MS). The median alpha (t(1/2alpha)), beta (t(1/2beta)), and gamma (t(1/2gamma)) half-lives were 0.33, 2.2, and 10.7 h respectively. The area under the DXM plasma concentration curve (AUC) was 113.5 ng.h/mL. At 72 h post-DXM administration, the plasma concentration of DXM in all horses was below the level of quantification (100 pg/mL). The baseline plasma concentration of COR was 3.5 +/- 0.69 ng/mL and declined significantly (P < 0.02) to 2.9 +/- 0.86 ng/mL at 1 h. The nadir in COR plasma concentration was 0.65 +/- 0.12 ng/mL at 28.8 +/- 9.0 h, and the DXM plasma concentration was 0.19 +/- 0.13 ng/mL. COR concentration returned to baseline at 96 h. Baseline plasma concentration of HYD was 58.8 +/- 11.7 ng/mL and declined significantly (P < 0.001) to 41.1 +/- 14.9 ng/mL at 1 h following DXM administration but recovered to baseline at 96 h. The sensitivity of LC/TSQ-MS/MS allowed complete description of the pharmacokinetics of DXM and its effect on plasma concentrations of both COR and HYD.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Pharmacokinetics of dexamethasone with pharmacokinetic/pharmacodynamic model of the effect of dexamethasone on endogenous hydrocortisone and cortisone in the horse

Soma

Uboh

Luo

et al. 2005

Vet Pharm & Therapeutics

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“…Both can be done quite effectively using LC/MS techniques. Among the drugs, dexamethasone and its metabolites have been determined ( 145), stilboestrol analogues( 146) using thermospray, trenbolone ( 147) and testosterone esters ( 148) in tissue and injectables. By LC/MS/MS, boldenone sulfonates and other conjugates ( 149) were determined.In a quantitative study using isotope dilution, budenoside metabolites ( 150) were measured using a moving belt interface.…”

Section: Steroidsmentioning

confidence: 99%

Analytical techniques for trace organic compounds - VI. Application of liquid chromatography-mass spectrometry (Technical Report)

Linscheid¹,

Westmoreland²

1994

Pure and Applied Chemistry

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Names of countries given after Members' names are in accordance with the IUPAC Handbook 1991-93; changes will be effected in the 1994-95 edition. Abstract -The development of combinations of liquid chromatography with mass spectroemtry in an on line fashion has come to a stage where such combinations become part of routine procedures for the analysis of a broad variety of samples. Since several technically very different interfaces have been devised in recent years, the major types are briefly discussed, even though it becomes more and more clear that only a few will survive. The most outstanding device appears to be the atmospheric pressure sources with electrospray and chemical ionization, since it covers many areas of chemical analysis in terms of polarity, lability and molecular weights. The attempt has been made not only to give a picture of the techniques, but to indicate by means of a few examples, how useful the different devices are and where they are at their limits. Furthermore, the criteiia are indicated which may be applied to make a choice on the basis of analytical requirements and technical and financial possibilities.

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Liquid chromatographic-mass spectrometric analysis of 2-nitrofluorene and its derivatives

Möller

Gustafsson

1986

Biol. Mass Spectrom.

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Liquid chromatography-mass spectrometry (LC-MS) with a nebuliser and a moving belt was used to characterise 2-nitrofluorene, its metabolites and related compounds. In total, 13 different compounds were analysed. The LC-MS interface did not affect the chromatographic performance achieved by high performance liquid chromatography (HPLC). The method allowed determination of the positions of the hydroxyl groups in different hydroxylated 2-acetylaminofluorene derivatives. The LC-MS procedure generated acceptable mass spectra with as small amounts of substance as 65 ng. The dose-response curves from the HPLC-detector and the ion-source in the LC-MS were parallel and linear.

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Qualitative detection of corticosteroids in equine biological fluids and the comparison of relative dexamethasone metabolite/dexamethasone concentration in equine urine by micro-liquid chromatography—mass spectrometry

Cited by 22 publications

References 13 publications

Pharmacokinetics of dexamethasone with pharmacokinetic/pharmacodynamic model of the effect of dexamethasone on endogenous hydrocortisone and cortisone in the horse

Pharmacokinetics of dexamethasone with pharmacokinetic/pharmacodynamic model of the effect of dexamethasone on endogenous hydrocortisone and cortisone in the horse

Analytical techniques for trace organic compounds - VI. Application of liquid chromatography-mass spectrometry (Technical Report)

Liquid chromatographic-mass spectrometric analysis of 2-nitrofluorene and its derivatives

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