Qualitative HPTLC phytochemical profiling of Careya arborea Roxb. bark, leaves and seeds

Abstract

Careya arborea Roxb. (Family: Lecythidaceae) is commonly called as Slow match tree and is an important medicinal plant. Its different parts viz. bark, leaves and seeds have been reported to show many pharmacological activities. High-performance thin-layer chromatography (HPTLC) is a simple, fast and precise technique for the detection of phytochemicals present in the plant. Therefore, the objective of the present study was to characterize the phytochemical profile for various secondary metabolites using HPTLC for C. arborea bark, leaves and seeds extracts, which revealed the confirmation of these phytochemicals. The present study suggested that the bark contains all the classes of compounds tested namely alkaloids, anthracene derivatives, arbutin derivatives, bitter compounds, cardiac glycosides, coumarin derivatives, essential oils, flavonoids, lignans, pungent-tasting principles, saponins, triterpenes and valepotriates. Whereas, alkaloids are not detected in leaves, and alkaloids and arbutin derivatives are not detected in seeds.

Screening for Astragalus hamosus Triterpenoid Saponins Using HPTLC Methods: Prior Identification of Azukisaponin Isomers

“…Saponins do not contain conjugated double bonds that can form chromophores, and are therefore difficult to detect under UV [ 43 , 44 , 45 , 46 , 47 ]. The use of a developer such as sulfuric anisaldehyde [ 48 ], allowed the saponins to take on blue, yellow, green, or purple colors [ 49 , 50 ].…”

New Drug Development strategies with special reference to the endocarp of multifaceted agriculture crop – Cocos nucifera Linn

“…The visualization of these developed plates were done under 254nm and 366 nm. The plates were then derivatized using Anisaldehyde reagent and further densitometry scanning was done under 254, 366 and 540 nm.CAMAG Linomat 5, CAMAG Reprostar 3andCAMAG Thin Layer Chromatography Scanner 3 were the HPTLC instrumentations employed in this study.HPTLC photo documentation, densitometry scan andcolour of distinct spots along with corresponding Rf values of the test sample were recorded as per standard procedures [10].…”