Qualitative Phytochemical Screening of Bologi (Senecio biafrae) and Bitter Leaf (Vernonia amygdalina) Leaves

Olasupo, A; Olagoke, O. V.; Aborisade, Abiodun Bukunmi

doi:10.9734/csji/2017/35753

Cited by 8 publications

(12 citation statements)

References 5 publications

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“…The appearance of this color indicates the presence of tannins. 12 Test of Flavonoids: 3ml of 1% Aluminium chloride solution was added to 5ml of each extract. A yellow coloration was observed indicating the presence of flavonoids.…”

Section: Test For Tanninsmentioning

confidence: 99%

“…5ml of dilute ammonia solution was added to the above mixture followed by addition of concentrated H2SO4.By adding it , the yellow coloration disappears which indicates the presence of flavonoids, thus , indicating the test positive for flavonoids. 12 Test for Saponin: About 5ml of the extract was boiled in 20ml of distilled water in a water bath and filtered. 10ml of the filterate was mixed with 5ml of distilled water and shaken vigorously for a stable persistent froth.…”

Section: Test For Tanninsmentioning

confidence: 99%

“…The frothing was mixed with 3 drops of olive oil and shaken vigorously, then observed for the formation of emulsion which confirms a positive presence of Saponin. 12 Phenol: 5ml of the extract was pipetted into a 30ml test tube, then 10ml of distilled water was added with 2ml of ammonium hydroxide solution and 5 ml of concentrated amyl alcohol and left to react for 30min. Development of bluish green colour indicates the presence of phenols , resulting into the positive test.…”

Section: Test For Tanninsmentioning

confidence: 99%

“…12 Test for Glycosides: To 1 ml of each extract, 0.5ml of glacial acetic acid and 3 drops of 1% aqueous ferric chloride solution were added, formation of brown ring at the interface indicates the presence of cardiac glycosides in the sample extract. 12 Test for Proteins: To 2 ml of each extract, 1 ml of 40% sodium hydroxide and few drops of 1% copper sulphate were added; formation of violet colour indicates the presence of peptide linkage molecules in the sample extract. 13 Test for Carbohydrates: Take 1 ml of extract, add few drops of Molisch's reagent and then add 1 ml of concentrated sulphuric acid at the side of the tubes.…”

Section: Test For Tanninsmentioning

confidence: 99%

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Antimicrobial Activity and Phytochemical Study of Plant Parts of Butea Monosperma

Dave¹,

Darji²,

Gandhi³

2019

J. Drug Delivery Ther.

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ABSTRACT: Since ancient times, plants have been model source of medicines as they are a reservoir of chemical agents with therapeutic properties. The general population is increasingly using herbal medicines as dietary supplements to relieve and treat many different human disorders. All parts of the plant, from root to fruit, consisting of a multitude of secondary metabolites which impart an unprecedented variety of medicinal uses to the plant. Studies have shown the presence of different phytochemical constituents in botanical sample responsible for the antimicrobial activity. These antimicrobial agents should be beneficial to host cells and toxic to pathogenic microbes. Hence, the antibacterial activity was examined from the leaf and flower of Butea monosperma. Sample was collected and its crude extract was obtained by using methanol, acetone and water as the extraction solvent. These extract were tested against some pathogenic microorganisms like Staphylococcus aureus , Bacillus cereusa and B. subtilis. The extract of Butea monosperma showed antibacterial activity against Staphylococcus aureus , Bacillus cereusa and B. subtilis. Bio-chemical test for the presence of phytochemicals have shown positive result and these phytochemicals have ability to fight against microorganisms or inhibit the growth of microorganisms. This approach will be an advanced step in the discovery of some herbal drugs. These plant extracts which was proven to be potentially effective can be used as a natural alternative to the chemical preservatives. It could be an ideal way to avoid health hazards that may occur due to chemical antimicrobial agents.

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Section: Test For Tanninsmentioning

confidence: 99%

Section: Test For Tanninsmentioning

confidence: 99%

Section: Test For Tanninsmentioning

confidence: 99%

Section: Test For Tanninsmentioning

confidence: 99%

See 2 more Smart Citations

Antimicrobial Activity and Phytochemical Study of Plant Parts of Butea Monosperma

Dave¹,

Darji²,

Gandhi³

2019

J. Drug Delivery Ther.

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“…Amaranthaceae species are characterized by the occurrence of different phytochemical compounds with bioactive properties (Müller & Borsch, 2005), but antibacterial activity, in particular, could be attributed to the presence of different flavonol sulfates (Chassagne et al, 2021). Spinach (Spinacia oleracea L.) is rich in different bioactive molecules and therefore known as an antioxidant, anti-inflammatory, antitumoral, antimutagenic, antineoplastic, chemopreventive, anti-obesity, and lipid-lowering agent (Adapa et al, 2018;Olasupo et al, 2018;Ribera et al, 2020). Chard (Beta vulgaris L. subsp.…”

Section: Introductionmentioning

confidence: 99%

Impact of Different Amaranthaceae Extracts on the Biofilm-Forming Capacity of Several Bacterial Pathogens

Mahmutović-Dizdarević

Salihović

Pazalja

et al. 2022

J microb biotech food sci

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Amaranthaceae Juss. family encompasses many edible plants with prominent biological activity. This investigation tested the bioactive properties of ethanolic and methanolic extract of three well-known species: spinach (Spinacia oleracea L.), chard (Beta vulgaris L. subsp. vulgaris), and orache (Atriplex hortensis L.) through the determination of total phenolic and flavonoid content, antioxidant activity, and antibacterial properties. The particular goal was to evaluate the antibiofilm potential of extracts and to demarcate concentration-depending changes in the biofilm-forming category of included bacterial strains. The mass of the chard and orache methanolic extracts gained by maceration are lower in comparison to the mass of ethanolic extracts obtained by the Soxhlet method. In the case of spinach, the results are the opposite. All extracts have an antiradical activity that can be attributed to the established amounts of phenols and flavonoids. Total phenolics in dry leaves ranged from 0.09 to 0.44 mg GAE/g dw, and total flavonoids from 0.42 to 1.9 mg RTE/g dw. All investigated extracts performed inhibitory potential in terms of bacterial growth, while there was no bactericidal effect observed. Values of the minimum inhibitory concentration ranged from 125 µg/ml to 500 µg/ml. Overall results suggested orache extracts as the strongest inhibitory agents. Antibiofilm assays showed that examined extracts of spinach, chard, and orache caused changes in the biofilm-forming capacity of investigated bacterial pathogens. Fluctuations in observed biofilm-forming categories after application of extracts were concentration-dependent.

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Metabolite Profiling and Cytotoxic Investigation ofSenecio biafraeOliv. & Hiern (Compositae) on Nauplii Brine Shrimps and Cancer Cell Lines

Akinola,

Oriyomi,

Ogundele

et al. 2023

Preprint

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The study investigated the cytoactivities of methanolic leaf extract (MLE) and fractions of Senecio biafrae, profiled, and elucidated their metabolites. Hydromethanolic extract of S. biafrae obtained by maceration was partitioned and subjected to cytotoxicity using brine shrimp lethality assay (BSLA), human cervical adenocarcinoma (HeLa) and prostate cancer cell (PC3) lines. The bioactive principles of most active fractions were profiled using GC-MS. The dichloromethane fraction (DMF) was purified using column and high-performance liquid chromatography to afford compounds 1 and 2 identified by 1H and 13C, DEPT-90, DEPT-135, COSY, NOESY and EIMS spectra. The MLE and its fractions exhibited good toxicity on nauplii brine shrimp. The HF exhibited a pronounced cytoactivity on PC-3 cell line with 92.8% followed by DMF. The GC-MS profiling of HF and DMF identified 17 and 6 constituents respectively comprising saturated and unsaturated fatty acids, diterpenoidal alcohol, phytol and aromatic compounds. Hexadecanoic acid, cis, cis, cis-9, 12, 15-octadecatrienoic acid, and phytol accounted for the highest percentage of the constituents in both fractions. Structure elucidation confirmed compounds 1 and 2 as stigmasterol and ergosterol with cytoactivity which compared favourably with doxorubicin. The study revealed that S.biafrae contains cytotoconstituents evidenced by proliferation inhibition of HeLa and PC-3 cell lines. The cyto-activities and bioconstituents indicate that S.biafrae has the potential to be considered as a candidate for drug development in the management of cancer-related conditions. Further investigation into the specific bioactive compounds and their mechanisms of action could provide valuable insights into the therapeutic potential of S.biafrae in cancer treatment.

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Qualitative Phytochemical Screening of Bologi (Senecio biafrae) and Bitter Leaf (Vernonia amygdalina) Leaves

Cited by 8 publications

References 5 publications

Antimicrobial Activity and Phytochemical Study of Plant Parts of Butea Monosperma

Antimicrobial Activity and Phytochemical Study of Plant Parts of Butea Monosperma

Impact of Different Amaranthaceae Extracts on the Biofilm-Forming Capacity of Several Bacterial Pathogens

Metabolite Profiling and Cytotoxic Investigation ofSenecio biafraeOliv. & Hiern (Compositae) on Nauplii Brine Shrimps and Cancer Cell Lines

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