Background Sprouting is one of the many processing techniques the food industry explores to improve the nutritional quality of cereals and other grains. Pro-vitamin biofortified maize alleviates Vitamin A deficiency and serves as an important source of antioxidants. This study aimed to determine the effect of sprouting on the physicochemical, antioxidant, and digestive enzyme inhibitory properties of pro-vitamin A biofortified maize. The maize grown at the International Institute of Tropical Agriculture (IITA) research field, Ibadan was initially subjected to analysis in which the proximate composition, functional properties, bioactive composition, antioxidant activity, and starch hydrolyzing inhibitory activity were carried out before sprouting. The samples of the sprouted maize were collected for different days (Day 1 to 4) and were further analyzed. Samples were further processed into donkwa, tuwo, and corn pudding using the traditional processing method, then analyzed for bioactive contents and blood glucose response in volunteers. Results obtained were analyzed using the SPSS software package and the mean values were compared.Results In the result, the proximate composition, functional properties, bioactive composition, antioxidant activity, and starch hydrolyzing inhibitory activity of the sprouted samples increased considerably compared to the non-sprouted (control). The proximate composition and digestive enzyme inhibitory activity of the samples increased with an increase in the day of sprouting. The bioactive composition of the day 3 sprouted sample varied significantly when compared with others including the control. The antioxidant activity of day 3 and 4 vary significantly compared to the control. The bioactive components in the products increased in their concentration with an increase in the day of sprouting. The blood glucose response in the volunteered subjects was higher in tuwo than donkwa and corn pudding.Conclusion The sprouting of provitamin A biofortified yellow maize enhanced antioxidants, protein, moisture, carbohydrates, hydrolyzing enzymes, and other essential nutritive substances.