Quality and in vitro fertilizing ability of cryopreserved cat spermatozoa obtained by urethral catheterization after medetomidine administration

Zambelli, Daniele; Prati, Francesca; Cunto, Marco; Iacono, Eleonora; Merlo, Barbara

doi:10.1016/j.theriogenology.2007.10.019

Cited by 94 publications

(115 citation statements)

References 16 publications

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“…Our findings are in agreement with the study of Zambelli et al (2008), in which no significant differences in the ability of in vitro production of embryos were found between cryopreserved urethral and electroejaculated spermatozoa. Our results are also supplementary to those of Filliers et al (2010), who showed that fertilizing ability was not different when fresh urethral and epididymal spermatozoa were used.…”

Section: Discussionsupporting

confidence: 93%

“…In this study we used commercial media designed for human in vitro embryoculture. Our results are comparable with these of other authors, who usually achieve the cleavage rate > 40%, and the morula and blastocyst rate between 20-60% (Freistedt et al 2001, Zambelli et al 2008, Thuwanut et al 2015. Therefore, we can conclude that the commercial human media used in our study occurred to be suitable for in vitro fertilization in cats.…”

Section: Discussionsupporting

confidence: 90%

“…From each tomcat urethral semen (CT) was collected by urethral catheterization after medetomidine administration (medetomidine hydrochloride im 100 μg/1 kg of body weight (Sedator 1.0 mg/ml, Novartis, Poland)) according to Zambelli et al (2008). Immediately after collection of urethral semen, ketamine im 5 mg/1 kg of body weight (VetKetam 100 mg/ml, VetAgro, Poland) was administered and orchiectomy performed.…”

Section: Semen Collection and Assessmentmentioning

confidence: 99%

“…One of the factors limiting the use of ART in felids in the past was difficulty in obtaining spermatozoa from tomcats. Collection of urethral semen proposed by Zambelli et al (2008) seems to solve this problem, but the characteristics of spermatozoa collected by this method are still poorly studied. Especially its fertilizing ability is not well understood, with only two papers concerning this subject (Zambelli et al 2008, Filliers et al 2010.…”

Section: Introductionmentioning

confidence: 99%

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In vitro fertilizing potential of urethral and epididymal spermatozoa collected from domestic cats (Felis catus)

Prochowska¹,

Niżański²

2017

Polish Journal of Veterinary Sciences

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The aim of this study was to provide a comparative analysis of in vitro fertilizing potential of frozen-thawed urethral and epididymal feline spermatozoa. Both types of semen were collected from 7 cats and cryopreserved in liquid nitrogen. To perform in vitro fertilization, both urethral and epididymal samples from the same individual were thawed and spermatozoa were co-incubated with in vitro matured cat oocytes. Obtained embryos were cultured in vitro for 7 days in a commercial medium. Cleavage rate, morula rate and blastocyst rate were calculated. Experiment was run in 10 replicates. The examined parameters showed no significant differences between urethral and epididymal spermatozoa (p>0.05). Cleavage rate and embryo's development were highly variable between replicates, even for the different sperm samples collected from one individual. There was no significant correlation between fertilizing capacity of two types of spermatozoa collected from the same male. In this study we confirmed that cryopreserved urethral spermatozoa have equally good fertilizing potential as epididymal ones, and both can be successfully used for in vitro fertilization in cats with the use of commercial medium.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionsupporting

confidence: 90%

Section: Semen Collection and Assessmentmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

In vitro fertilizing potential of urethral and epididymal spermatozoa collected from domestic cats (Felis catus)

Prochowska¹,

Niżański²

2017

Polish Journal of Veterinary Sciences

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“…1,2 Urethral catheterisation after pharmacological induction with medetomidine allows the collection of semen characterised by low volume and a high concentration of spermatozoa; moreover, the sperm cells are suitable for cryopreservation or in vitro fertilisation. 3 For the purpose of semen collection, medetomidine was formerly used at a dose of 130-140 µg/kg, but because of the possible side effects on the cardiovascular system, the pharmacological induction of sedation/anaesthesia with medetomidine still represents a limitation because of the risks for the patient. In fact, as reported by Romagnoli et al, 4 a dose of 130 µg/kg medetomidine is responsible for significant haemodynamic effects on the feline heart, such as the reduction in heart rate, and increased cardiac preload and impaired systolic function.…”

Section: Introductionmentioning

confidence: 99%

Usefulness of an injectable anaesthetic protocol for semen collection through urethral catheterisation in domestic cats

Pisu¹,

Ponzio

Rovella³

et al. 2016

Journal of Feline Medicine and Surgery

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Objectives Although less often requested in comparison with dogs, the collection of semen in cats can be necessary for artificial insemination, for semen evaluation in tom cats used for breeding and for semen storage. Urethral catheterisation after pharmacological induction with medetomidine has proved to be useful for the collection of semen in domestic cats. However, most of the previously used protocols require the administration of high doses of medetomidine that can increase the risk of side effects, especially on the cardiovascular system. In routine clinical practice, one safe and useful injectable anaesthetic protocol for short-term clinical investigations or surgery in cats involves premedication with low intramuscular doses of dexmedetomidine with methadone, followed by intravenous propofol bolus injection. We aimed to assess the usefulness of this injectable anaesthetic protocol for semen collection, via urethral catheterisation, in domestic cats. Methods The study was performed on 38 purebred, adult cats, during the breeding season, and semen was collected via urethral catheterisation using an injectable anaesthesia protocol with methadone (0.2 mg/kg) and dexmedetomidine (5 µg/kg) premedication, followed by induction with propofol. Results The anaesthetic protocol used in the present study allowed the collection of large-volume semen samples, characterised by good parameters and without side effects. Conclusions and relevanceThe results from the present study suggest that the injectable anaesthetic protocol using methadone and dexmedetomidine premedication, followed by induction with propofol, could be suitable and safe for the collection of a good-quality semen sample, via urethral catheterisation, in domestic cats. It can therefore be used as an alternative to previous medetomidine-based sedation protocols.

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Spermatic profile of captive giant anteaters (Myrmecophaga tridactyla): Knowing more to preserve better

et al. 2021

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The giant anteater (Myrmecophaga tridactyla) is being threatened by natural habitat destruction and fragmentation, illegal hunting and road kills. In this context, the generation of basic information on the reproductive parameters of this species is vital, aiming to improve reproductive management via, amongst others, assisted reproductive technologies. This study aimed to describe the morphological and functional features of semen collected from captive giant anteaters. Electroejaculation was performed in 13 animals housed in zoos located in São Paulo state, Brazil. Semen samples were collected from 13 animals in 16 procedures. Samples were evaluated for volume, motility, vigor, pH, concentration, sperm morphology, and functional tests. The following mean values were obtained: volume 1.28 ± 0.27 mL; motility 28.3 ± 6.2%; vigor 2.4 ± 0.25; concentration 129.4 ± 36.1 × 106 sperm/mL; pH 7.4 ± 0.2. Total acrosome, head, midpiece, and tail sperm abnormalities were 3.2 ± 0.8%, 25.4 ± 3.6%, 20.7 ± 3.2%, and 14.7 ± 2.6%, respectively. Intact acrosome was found in 83.7 ± 3.1% and intact membrane in 81.1 ± 4.0% of all samples collected. Mitochondrial activity was 66.4 ± 6.0% (Class I), 18.7 ± 2.9% (Class II), 8.0 ± 2.0% (Class III), 3.9 ± 1.0% (Class IV), and 3.0 ± 0.9% (Class V). Sperm DNA fragmentation rate was 13.2 ± 3.7%. These results indicated that electroejaculation is a feasible method for semen collection in giant anteaters, allowing a more detailed description of the semen in this species.

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Quality and in vitro fertilizing ability of cryopreserved cat spermatozoa obtained by urethral catheterization after medetomidine administration

Cited by 94 publications

References 16 publications

In vitro fertilizing potential of urethral and epididymal spermatozoa collected from domestic cats (Felis catus)

In vitro fertilizing potential of urethral and epididymal spermatozoa collected from domestic cats (Felis catus)

Usefulness of an injectable anaesthetic protocol for semen collection through urethral catheterisation in domestic cats

Spermatic profile of captive giant anteaters (Myrmecophaga tridactyla): Knowing more to preserve better

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